Techniques for neuropeptide determination

Sandberg, Mats; Weber, Stephen G.

doi:10.1016/s0165-9936(03)00910-5

Cited by 15 publications

(12 citation statements)

References 48 publications

(43 reference statements)

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“…Robust fractionation techniques, such as two-dimensional gel electrophoresis, liquid chromatography, and affinity purifications, are commonly used in conjunction with MS to enhance investigation at the protein level. Separated analytes can be detected using UV absorbance, fluorescence, electrochemical, nuclear magnetic resonance (NMR), and MS methods (Sandberg and Weber, 2003). The first three approaches typically demand analyte standards for SP identification, while NMR and MS are capable of identifying molecules directly.…”

Section: Analytical Technologies For Discovery and Characterizatiomentioning

confidence: 99%

Characterizing intercellular signaling peptides in drug addiction

et al. 2009

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Intercellular signaling peptides (SPs) coordinate the activity of cells and influence organism behavior. SPs, a chemically and structurally diverse group of compounds responsible for transferring information between neurons, are broadly involved in neural plasticity, learning and memory, as well as in drug addiction phenomena. Historically, SP discovery and characterization has tracked advances in measurement capabilities. Today, a suite of analytical technologies is available to investigate individual SPs, as well as entire intercellular signaling complements, in samples ranging from individual cells to entire organisms. Immunochemistry and in situ hybridization are commonly used for following preselected SPs. Discovery-type investigations targeting the transcriptome and proteome are accomplished using high-throughput characterization technologies such as microarrays and mass spectrometry. By integrating directed approaches with discovery approaches, multiplatform studies fill critical gaps in our knowledge of drug-induced alterations in intercellular signaling. Throughout the past 35 years, the National Institute on Drug Abuse has made significant resources available to scientists that study the mechanisms of drug addiction. The roles of SPs in the addiction process are highlighted, as are the analytical approaches used to detect and characterize them.

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Section: Analytical Technologies For Discovery and Characterizatiomentioning

confidence: 99%

Characterizing intercellular signaling peptides in drug addiction

et al. 2009

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“…Detection and quantitation of small molecules is used to check the quality of vast category of soft drinks, fruit juices and other perishable drinks . Short peptides lacking aromatic amino acids and neuropeptides like substance P and Leu‐enkephalin can be detected by fluorophore conjugation ,. Fluorophore conjugation is useful in detection of low quantities of polyamines, natural amines and amines based drugs in biological fluids .…”

Section: Introductionmentioning

confidence: 99%

Optimization of Ion‐Dependent Green Synthesis of Fmoc‐Amino Acids in Phosphate Buffer

et al. 2017

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Here we show the optimization of Fmoc amino acid conjugation reaction in physiological relevant phosphate buffer using ethanol as a green solvent. Using Phenylalanine as model amino acid, different parameters including water, temperature, stirring, pH, buffer composition, ions and solvents were tested to optimize the product yield. Using the optimized method, Fmoc conjugation of other amino acids and a dipeptide are reported. Important role of buffer ions in the catalyst free Fmoc‐Phenylalanine synthesis and implication of phosphate ions through 31P Nuclear Magnetic Resonance (NMR) in the product formation have been demonstrated. The characterization of the final products was carried using reverse phase chromatography, mass spectrometry and NMR spectroscopy. Optimized method using phosphate buffer and ethanol as green solvent might provide substitute for borate buffer and pave the way for amino acid detection in biological fluids under physiologically relevant and environmental friendly conditions.

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“…Analogous considerations can be smoothly switched also to detectors based on fluorescence [6,7] and chemiluminescence [8,9] processes. In this scenario, the employment of , mass-sensitive' detectors is strictly recommended and the selection is principally pursued by considering the cost-benefit compromise.…”

Section: Introductionmentioning

confidence: 99%

Side‐chain modified bile acids: chromatographic separation of 23‐methyl epimers

Natalini¹,

Sardella

Gioiello

et al. 2009

J of Separation Science

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Owing to the re-flourished interest towards the bile acids (BAs) as versatile signalling hormones endowed with diverse endocrine functions, the development of reliable analytical protocols monitoring the synthesis of new BA-based receptor modulators, still represents a cogent concern. On this basis, for the first time, a HPLC study has been engaged with the aim to set up suitable chromatographic conditions for the analysis of three different epimeric couples of 23-methyl-substituted unconjugated BAs. Three different methods (one for each couple) have been successfully established and then validated. Good precision and accuracy (evaluated both in the short and long period) as well as appreciably low LOD and LOQ values have turned out. Moreover, the engagement of an evaporative light scattering detector (ELSD) has proven its high effectiveness for the analysis of such steroidal species.

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Techniques for neuropeptide determination

Cited by 15 publications

References 48 publications

Characterizing intercellular signaling peptides in drug addiction

Characterizing intercellular signaling peptides in drug addiction

Optimization of Ion‐Dependent Green Synthesis of Fmoc‐Amino Acids in Phosphate Buffer

Side‐chain modified bile acids: chromatographic separation of 23‐methyl epimers

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