Capillary electrochromatography of proteins and peptides (2006–2015)

Mikšı́k, Ivan

doi:10.1002/jssc.201600908

Cited by 46 publications

(18 citation statements)

References 134 publications

(187 reference statements)

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“…CEC did not become a widely used method for peptide separations and analyses. The developments and applications of CEC to separation and analysis of peptides and proteins in the last decade were recently summarized in a comprehensive review by Miksik . In comparison with MEKC, the advantage of CEC is that the composition of its mobile phase is more compatible with on‐line coupling to MS detection.…”

Section: Separations By the Particular Ce And Cec Methodsmentioning

confidence: 99%

Recent developments in capillary and microchip electroseparations of peptides (2015–mid 2017)

Kašička

2017

Electrophoresis

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The review brings a comprehensive overview of recent developments and applications of high performance capillary and microchip electroseparation methods (zone electrophoresis, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography, and electrochromatography) to analysis, microscale isolation, purification, and physicochemical and biochemical characterization of peptides in the years 2015, 2016, and ca. up to the middle of 2017. Advances in the investigation of electromigration properties of peptides and in the methodology of their analysis (sample preseparation, preconcentration and derivatization, adsorption suppression and EOF control, and detection) are described. New developments in particular CE and CEC methods are presented and several types of their applications to peptide analysis are reported: qualitative and quantitative analysis, determination in complex (bio)matrices, monitoring of chemical and enzymatical reactions and physical changes, amino acid, sequence and chiral analysis, and peptide mapping of proteins. Some micropreparative peptide separations are shown and capabilities of CE and CEC methods to provide important physicochemical characteristics of peptides are demonstrated.

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Section: Separations By the Particular Ce And Cec Methodsmentioning

confidence: 99%

Recent developments in capillary and microchip electroseparations of peptides (2015–mid 2017)

Kašička

2017

Electrophoresis

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“…To improve the reproducibility, the preconditioning of the capillary should be performed. Open tubular capillary electrochromatography (OT‐CEC), combining the high separation efficiency of CE and high selectivity of LC, has demonstrated superiority in proteins separation due to the advantages including simple column preparation, no bubble formation, and simple instrumental handling . More importantly, the coatings in CEC can effectively restrain the adsorption of proteins on the inner surface of columns and improve the resolution of proteins separation .…”

Section: Introductionmentioning

confidence: 99%

Poly(norepinephrine)‐coated open tubular column for the separation of proteins and recombination human erythropoietin by capillary electrochromatography

Xue

Zhang

et al. 2017

J of Separation Science

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Recombinant human erythropoietin is an important therapeutic protein with high economic interest due to the benefits provided by its clinical use for the treatment of anemias associated with chronic renal failure and chemotherapy. In this work, a poly(norepinephrine)‐coated open tubular column was successfully prepared based on the self‐polymerization of norepinephrine under mild alkaline condition, the favorable film forming and easy adhesive properties of poly(norepinephrine). The poly(norepinephrine) coating was characterized by scanning electron microscopy and measurement of the electro‐osmotic flow. The thickness of the coating was about 431 nm. The electrochromatographic performance of the poly(norepinephrine)‐coated open tubular column was evaluated by separation of proteins. Some basic and acidic proteins including two variants of bovine serum albumin and two variants of β‐lactoglobulin achieved separation in the poly(norepinephrine)‐coated open tubular column. More importantly, the column demonstrated separation ability for the glycoforms of recombinant human erythropoietin. In addition, the column demonstrated good repeatability with the run‐to‐run, day‐to‐day, and column‐to‐column relative standard deviations of migration times of proteins less than 3.40%.

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“…From this instrumentation, it is possible to mention commercially available interfaces: coaxial sheath‐flow interface G1607A (Agilent Technologies, USA), porous tip interface, CESI 8000 (AB SCIEX, USA), electrospray emitter with EOF driven sheath liquid, EMASSII (Prince Technologies, Netherlands) and interface CEi SP20 (Reeko Instrument, Xiamen, China) developed for combination of ICP with MS. To date, there is a plethora of books, book chapters, and review articles about the use of coupling capillary electrophoretic methods with MS in various fields of application and separation science, e.g. . The use of CE and MS is still of great interest, as evidenced by the statistic from the Web of Science (Clarivate Analytics).…”

Section: Introductionmentioning

confidence: 99%

Coupling of CE‐MS for protein and peptide analysis

Mikšı́k

2018

J of Separation Science

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The review is focused on the latest developments in the analysis of proteins and peptides by capillary electrophoresis techniques coupled to mass spectrometry. First, the methodology and instrumentation are overviewed. In this section, recent progress in capillary electrophoresis with mass spectrometry interfaces and capillary electrophoresis with matrix‐assisted laser desorption/ionization is mentioned, as well as separation tasks. The second part is devoted to applications—mainly bottom‐up and top‐down proteomics. It is obvious that capillary electrophoresis with mass spectrometry methods are well suited for peptide and protein analysis (proteomic research) and it is described how these techniques are complementary and not competitive with the often used liquid chromatography with mass spectrometry methods.

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Capillary electrochromatography of proteins and peptides (2006–2015)

Cited by 46 publications

References 134 publications

Recent developments in capillary and microchip electroseparations of peptides (2015–mid 2017)

Recent developments in capillary and microchip electroseparations of peptides (2015–mid 2017)

Poly(norepinephrine)‐coated open tubular column for the separation of proteins and recombination human erythropoietin by capillary electrochromatography

Coupling of CE‐MS for protein and peptide analysis

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