Capillary electrochromatography of basic compounds using octadecyl-silica stationary phases with an amine-containing mobile phase

Hilhorst, M.J; Somsen, G.W; Jong, GJ de

doi:10.1016/s0021-9673(99)01325-4

Cited by 35 publications

(14 citation statements)

References 17 publications

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“…As the addition of a competing amine to the mobile phase at low pH is an effective approach to solve the peak tailing in HPLC, Lurie et al [12] first applied this method for separation of basic solutes in reversed-phase CEC, and both the peak shape and column efficiency for basic solutes were significantly improved. Other reports also confirmed their results [13,14]. Alternative solutions to the problem of tailing peaks are the use of other types of stationary phases.…”

supporting

confidence: 53%

Determination of basic pharmaceuticals in human serum by hydrophilic interaction capillary electrochromatography

Jin

Xiao

et al. 2004

Electrophoresis

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Hydrophilic interaction capillary electrochromatography (HI-CEC) for the determination of basic pharmaceuticals spiked in human serum is described. The organic modifier content, ionic strength, and pH value of the mobile phase as well as the applied voltage are optimized for separation and elution of these drug analytes. Excellent separation was achieved for drugs using a mobile phase composition of 80% v/v acetonitrile in 100 mM triethylamine phosphate (TEAP) buffer at pH 2.8 with column efficiencies for analytes more than 200,000 plates/m. The samples of human serum spiked with basic drugs were directly injected after a simple acetonitrile treatment. The linear range and reproducibility of these basic drugs using an external and internal standard method were compared. As a result, the reproducibility could be greatly improved by using the internal standard method. Good calibration curves with regression coefficients more than 0.998 in the range of 5-160 microg/mL were observed with the internal standard method. The limits of quantitation, based on standards with acceptable relative standard deviations (RSDs), were below 5 microg/mL. The intra- and inter-day precisions, determined as RSDs, were less than 4.57%.

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supporting

confidence: 53%

Determination of basic pharmaceuticals in human serum by hydrophilic interaction capillary electrochromatography

Jin

Xiao

et al. 2004

Electrophoresis

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“…While long analysis times are encountered in the case of acidic compounds in silicabased CEC columns, due to the different direction of their electrophoretic mobility from EOF. Some approaches have now been undertaken to overcome these problems [29,[44][45][46][47]. In order to improve the peak symmetry of basic compounds, a conventional method would be to add a competing base to the mobile phase to compete with the analytes [44][45][46].…”

Section: Separation Of Different Types Of Samples On the Monolithic Cmentioning

confidence: 99%

Optimized preparation of poly(styrene‐co‐ divinylbenzene‐co‐methacrylic acid) monolithic capillary column for capillary electrochromatography

Jin

Huang

et al. 2003

Electrophoresis

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Preparation of a poly(styrene-co-divinylbenzene-co-methacrylic acid) monolithic stationary phase for the use in capillary electrochromatography (CEC) has been improved by optimizing the polymerization conditions. It is observed that the reaction time strongly affects column efficiency, while the proportion of isooctane in porogen influences peak symmetry of some solutes seriously. The lifetime of the monolithic columns prepared mainly depends on the pH of buffers used. Reproducibility of electroosmotic flow (EOF) from batch to batch columns are lower than 2.8% relative standard deviation. Unlike other types of capillary electrochromatographic monoliths, a pH-dependent EOF was observed on this type of column. Separation of various types of compounds including aromatic hydrocarbons, hormones, anilines, basic pharmaceuticals, and peptides was achieved. The facile preparation and wide application of this monolithic column may make styrene-based polymer a potential stationary phase in CEC.

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“…The first reported use of amine modifier was shown by Gillot et al [421]. A similar approach was also applied to Hypersil and Waters stationary phases by Dittmann et al [89] and Hilhorst et al [418]. Ru et al [230] used pressurized gradient CEC for the analysis of eighteen amino-acid derivatives on a Unimicro C18 stationary phase.…”

Section: Common Reversed-phase Stationary Phasesmentioning

confidence: 99%

Stationary and mobile phases in capillary electrochromatography (CEC)

Jiskra

Claessens²,

Cramers

2003

J of Separation Science

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Stationary and mobile phases in capillary electrochromatography (CEC)This review describes the state of the art of capillary electrochromatography (CEC). Properties of and interactions between stationary and mobile phases applied in CEC are described and discussed; developments in stationary phases, partly also monolithic stationary phases, are reviewed. Special attention is paid to the comparison of the behaviour of stationary and/or mobile phases under CEC versus HPLC conditions with respect to variables such as particle and pore size of the stationary phase, mobile phase composition, and temperature. These issues are discussed throughout the paper. A number of applications in CEC is presented as well.

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Capillary electrochromatography of basic compounds using octadecyl-silica stationary phases with an amine-containing mobile phase

Cited by 35 publications

References 17 publications

Determination of basic pharmaceuticals in human serum by hydrophilic interaction capillary electrochromatography

Determination of basic pharmaceuticals in human serum by hydrophilic interaction capillary electrochromatography

Optimized preparation of poly(styrene‐co‐ divinylbenzene‐co‐methacrylic acid) monolithic capillary column for capillary electrochromatography

Stationary and mobile phases in capillary electrochromatography (CEC)

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