Capillary Electrochromatography-Electrospray Mass Spectrometry: A Microanalysis Technique

Schmeer, Karl; Behnke, Beate; Bayer, Ernst

doi:10.1021/ac00116a007

Cited by 136 publications

(65 citation statements)

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“…In particular their simple coupling with separation techniques such as HPLC, [6] capillary electrophoresis (CE), [7] and capillary electrochromatography (CEC) [8] is the method of choice for characterization, analysis, and structural elucidation. A characteristic common to these techniques is the formation of droplets from which ions are subsequently liberated.…”

Section: Coordination-ionspray-ms (Cis-ms) a Universal Detection Andmentioning

confidence: 99%

Coordination-Ionspray-MS (CIS-MS), a Universal Detection and Characterization Method for Direct Coupling with Separation Techniques

Bayer

Gfrörer

Rentel

1999

Angew. Chem. Int. Ed.

115

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By on-line addition of a central atom (for example, Ag , B , Pd , Li ) positively or negatively charged complexes of analytes can be formed for CIS-MS. This technique is applicable to both polar and nonpolar compounds-for example, for alcohols, ethers, and a large number of olefins, polyolefins, and arenes as well as steroids, vitamins of the D and E families, carotinoids, polystyrols, terpenes, and unsaturated fatty acids-and can be readily coupled with separation techniques.

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Section: Coordination-ionspray-ms (Cis-ms) a Universal Detection Andmentioning

confidence: 99%

Coordination-Ionspray-MS (CIS-MS), a Universal Detection and Characterization Method for Direct Coupling with Separation Techniques

Bayer

Gfrörer

Rentel

1999

Angew. Chem. Int. Ed.

115

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“…[1][2][3][4][5][6][7] This promising technique makes use of ESI-MS to monitor the rapid, high resolution separations achieved during CZE. The small sample sizes associated with CZE necessitate the most sensitive mass spectrometric detection and identification techniques available.…”

Section: Received 4 October 1998; Accepted 5 November 1998mentioning

confidence: 99%

A novel nanospray capillary zone electrophoresis/mass spectrometry interface

Hsieh

Baronas

Muir

et al. 1999

Rapid Commun. Mass Spectrom.

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The high resolution of capillary zone electrophoresis/mass spectrometry (CZE/MS) offers a promising technique to characterize biomolecules in pharmaceutical and biotechnology industries. A novel capillary zone electrophoresis/electrospray ionization time-of-flight mass spectrometry (CZE/ESI-TOFMS) interface was designed in this study to successfully integrate ESI-TOFMS, nanospray, and CZE for biomolecular identification. The interface offers a novel way to take advantage of the high resolution separation achieved during CZE and the detection sensitivity of nanospray ESI-MS. The results showed mixtures of peptides were highly resolved within a few minutes (each CZE electropherogram of a peptide is 2-3 seconds). The novel CZE/ESI-TOFMS interface may simultaneously provide sensitivity, data acquisition speed, mass range, and mass resolution while maintaining resolution of the CZE separation.

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“…To solve this problem, buffers with low ionic strength are commonly used to decrease the Joule heat. In addition, supplementary pressure could be applied on the column, which is called pressurized or pressureassisted CEC (pCEC) [4][5][6][7][8][9][10]. Although UV detectors are generally used in CEC, mass spectrometry (MS) is a powerful detection method that could provide high sensitivity and reliable detection for various samples, particularly in biochemical applications [11][12][13].…”

Section: Introductionmentioning

confidence: 99%

On‐line concentration of proteins in pressurized capillary electrochromatography coupled with electrospray ionization‐mass spectrometry

et al. 2005

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Pressurized capillary electrochromatography (pCEC) and electrospray ionization-mass spectrometry (ESI-MS) have been hyphenated for protein analysis. Taken cytochrome c, lysozyme, and insulin as samples, the limits of detection (LODs) for absolute concentrations are 10 211 mol (signal-to-noise ratio S/N = 3) with relative standard deviations (RSDs) of retention time and peak area, respectively, of less than 1.7% and 4.8%. In order to improve the detection sensitivity, on-line concentration by fieldenhanced sample-stacking effect and chromatographic zone-sharpening effect has been developed, and parameters affecting separation and detection, such as pH and electrolyte concentration in the mobile phase, separation voltage, as well as enrichment voltage and time, have been studied systematically. Under the optimized conditions, the LODs of the three proteins could be decreased up to 100-fold. In addition, the feasibility of such techniques has been further demonstrated by the analysis of modified insulins at a concentration of 20 mg/mL.

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Capillary Electrochromatography-Electrospray Mass Spectrometry: A Microanalysis Technique

Cited by 136 publications

References 0 publications

Coordination-Ionspray-MS (CIS-MS), a Universal Detection and Characterization Method for Direct Coupling with Separation Techniques

Coordination-Ionspray-MS (CIS-MS), a Universal Detection and Characterization Method for Direct Coupling with Separation Techniques

A novel nanospray capillary zone electrophoresis/mass spectrometry interface

On‐line concentration of proteins in pressurized capillary electrochromatography coupled with electrospray ionization‐mass spectrometry

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