2023
DOI: 10.1039/d3ay00225j
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Capillary driven microfluidic sequential flow device for point-of-need ELISA: COVID-19 serology testing

Abstract: A capillary-driven microfluidic sequential flow device, designed for eventual at-home or doctor’s office use, was developed to perform an enzyme-linked immunosorbent assay (ELISA) for serology assays. Serology assays that detect...

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Cited by 6 publications
(3 citation statements)
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“…32 Similarly, the research group of Charles Henry established a capillary-driven immunoassay (CaDI) system, which allows the automation of serological assays, among many others, by sequentially delivering reagents to a detection zone. 33,34 In their system, all assay reagents are pre-stored in dried form on the microfluidic device within glass fiber reagent pads which are reconstituted in solution at particular concentrations after sample/buffer application. Despite being extremely promising for automating complex multistep bioassay, these capillary flow-based systems only allow successive delivery of different reagents to a particular detection region.…”
Section: Introductionmentioning
confidence: 99%
“…32 Similarly, the research group of Charles Henry established a capillary-driven immunoassay (CaDI) system, which allows the automation of serological assays, among many others, by sequentially delivering reagents to a detection zone. 33,34 In their system, all assay reagents are pre-stored in dried form on the microfluidic device within glass fiber reagent pads which are reconstituted in solution at particular concentrations after sample/buffer application. Despite being extremely promising for automating complex multistep bioassay, these capillary flow-based systems only allow successive delivery of different reagents to a particular detection region.…”
Section: Introductionmentioning
confidence: 99%
“…When the liquid sample is injected into the sample pad, the flow is generated by the capillary force of the paper, and the flow proceeds through the gold pad toward the NC membrane and the absorbent pad. 23,24 To indicate the detection results, the LFA strip utilizes various labels such as gold nanoparticles, carbon nanoparticles, fluorescent nanoparticles, latex beads, selenium, colloidal carbon, and quantum dots. 25,26 Gold nanoparticles are easy to functionalize and have optical properties that appear when the density per unit area increases; thus, the results can be confirmed with naked eye without any additional equipment to check the detection results.…”
Section: Introductionmentioning
confidence: 99%
“…CuNSs were exploited to improve electrode surface area (1.2 times), elevate active sites for antibody confinement, and enrich electrocatalytic property of ruthenium redox species, consequently resulting in a 0.02pg/mL of LOD. In addition to these detection schemes, integrated electrochemical sensor microfluidic devices have been introduced to detect both single and multiple analytes simultaneously [16,20]. For example, Naorungroj et al [19] presented a G-quadruplex DNAzyme-based electrochemical sensor integrated with a sequential flow controllable microfluidic device for the detection of COVID-19.…”
mentioning
confidence: 99%