Capillary-based chemiluminescence immunoassay for C-reactive protein with portable imaging device

Abstract

A capillary-based chemiluminescence immunoassay system using a charge-coupled device (CCD) camera as detector was established in this paper. The fused quartz capillary was easily activated in one step for immobilizing capture antibody, and the chemiluminescence immunoassay was carried out in the capillary in double-antibody sandwich format. Chemiluminescence signals were recorded by the portable imaging device which was installed with the CCD camera and the results were analyzed through gray intensity. The total cost time, which included not only the time for test but also the time for the preparation of experimental materials, was only 2 h. The immunoassay was performed without any complicated or expensive instruments. The consumption of the sample was only 0.8 μL in one test, which was significantly less than other methods. In this work, C-reactive protein (CRP), as a target, was quantitatively detected from 0.3 to 160.0 μg mL with high specificity and low sample volume. The reproducibility and accuracy were tested in clinic human serum samples and shown good results. Thus, this rapid, easy preparation and using, portable immunoassay system indicated its usefulness as a novel technology platform. Graphical abstract ᅟ.

Ultrasensitive fluorescent aptasensor for CRP detection based on the RNase H assisted DNA recycling signal amplification strategy

“…As presented in Table 1, CRP detection performance of our strategy was comparable to the reported in most of the other studies. [3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18]…”

“…2 The most widely used CRP diagnostic methods are antibody-based assays. 2 At present, several techniques including the use of a capacitive biosensor, 3 microuidic biosensor, 4-6 electrochemical biosensor, [7][8][9] sandwich immunoassay, 10,11 uorescent biosensor, 12 chemiluminescence immunoassay, 13 micro-uxgate sensor, 14 ber optic biosensor, 15 magnetoimpedancebased biosensor, 16 nanowire-mesh biosensor 17 and electrochemical impedance spectroscopy 18 have been developed for the detection of CRP. However, these methods for CRP detection are usually time-consuming, labor-intensive, complex in operation or preparation and involve expensive reagents.…”

Microfluidic on-chip valve and pump for applications in immunoassays

“…We capture the CL images using a homemade portable CL imager. 43 The camera of the imager has a Sony ICX 825 image sensor. We use ImageJ (National Institutes of Health, USA) and Origin 8.0 (Origin Lab, USA) to analyze the images and data.…”

Development of a chemiluminescence immunoassay to accurately detect African swine fever virus antibodies in serum

“…The chemiluminescence immunoassay (CLIA) was proposed by Halman et al in 1977 (12). CLIA, which combines a highly sensitive chemiluminescence reaction system (which was not certified by peer review) is the author/funder.…”

“…The chemiluminescence immunoassay (CLIA) was proposed by Halman et al in 1977 (12). CLIA, which combines a highly sensitive chemiluminescence reaction system with a highly specific immune response system, is currently the most widely used analytical technique (13).…”