Ultrasensitive fluorescent aptasensor for CRP detection based on the RNase H assisted DNA recycling signal amplification strategy

Abstract

An aptamer-based method for the ultrasensitive fluorescence detection of C-reactive protein (CRP) was developed using the ribonuclease H (RNase H) assisted DNA recycling signal amplification strategy.

Electrochemical detection of C-reactive protein using functionalized iridium nanoparticles/graphene oxide as a tag

“…A comparison of different immunoassay for the determination of CRP was shown in Table 1. [23][24][25][26] The results indicated that the linear range of this immunosensor was better than that of the previous reports.…”

Magnetic-enhanced fluorescence sensing of tumor miRNA by combination of MNPs@PDA with duplex specific nuclease

“…Fluorescence spectroscopy (FS) has the advantages of simple operation, low-cost, and high selectivity. Based on the characteristics of nanomaterials (graphene oxide, 7 carbon nanotube, WS 2 , 8 molybdenum disulfide 9 ), they have been widely used in FS for the detection of biomarkers (DNA, 8 miRNA, 10 proteins, 11 small molecules 12 and exosomes 13 ). In principle, these biomarkers are adsorbed on nanomaterials through π–π stacking and hydrogen bond.…”

Covalent and Noncovalent Functionalization of Graphene Oxide with DNA for Smart Sensing

“…Similar sensors have also been developed for other targets, such as C-reactive protein, tropomyosin, and Hg 2þ , with the latter being implemented in a "signal on" variation. [27,95,96] Figure 6. A) General scheme for a GO/DNA biosensor designed with an internal reference DNA.…”

“…Similar sensors have also been developed for other targets, such as C‐reactive protein, tropomyosin, and Hg 2+ , with the latter being implemented in a “signal on” variation. [ 27,95,96 ]…”