CAPE increases the expression of SOD3 through epigenetics in human retinal endothelial cells

Ohashi, Atsuko; Yasuda, Hiroyuki; Kamiya, Tetsuro; Hara, Hirokazu; Adachi, Tetsuo

doi:10.3164/jcbn.16-109

Cited by 12 publications

(7 citation statements)

References 58 publications

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“…SOD3 is known as a predominant form of SODs in plasma [ 10 ]. In many studies, it has been shown that SOD3 is mainly expressed in endothelial cells [ 11 , 26 , 27 ], and its decrease was associated with vascular endothelium damage and the infiltration of inflammatory cells [ 5 , 10 , 28 ]. In our previous study [ 29 ], we demonstrated an increased concentration of endothelium damage markers in AP patients which could be associated with decreased SOD3 concentration in the plasma of these patients.…”

Section: Discussionmentioning

confidence: 99%

Changes in the Activity and Concentration of Superoxide Dismutase Isoenzymes (Cu/Zn SOD, MnSOD) in the Blood of Healthy Subjects and Patients with Acute Pancreatitis

et al. 2020

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This study was aimed at evaluating the changes in the concentration and activity of all superoxide dismutase isoenzymes (SOD1, SOD2, SOD3) in the blood of patients with acute pancreatitis (AP) and healthy subjects, taking into account the extracellular (plasma) and intracellular (erythrocyte lysate) compartment. The relationships between the activity/concentration of SODs, metal concentration and the markers of inflammation were evaluated. To assess the pro/antioxidative imbalance, the malonyldialdehyde (MDA) concentration and the value of total antioxidant capacity (TAC) were measured. The impact of single-nucleotide polymorphism (SNP) in the SOD1 gene (rs2070424) on the activity/concentration of SOD1 as the main isoenzyme of the SOD family was also analyzed in this study. The SOD2 activity in erythrocytes was increased compared to plasma: 10-fold in the AP patient group and 5-fold in healthy subjects. The plasma of AP patients showed an increased SOD1 concentration and decreased SOD2 and SOD3 concentrations compared to healthy subjects. The Cu/Zn SOD (SOD1 + SOD3) concentration in plasma of AP patients was elevated compared to healthy subjects, but changes in plasma Cu/Zn SOD (SOD1 + SOD3) activity in the examined groups were not observed. An influence of SNP rs2070424 in the SOD1 gene on the total activity of SOD in AP patients (with AG genotype), accompanied by an increased IL-6 concentration, was observed. In oxidative stress conditions induced by inflammation, the participation of individual forms of plasma SOD isoenzymes in total antioxidative activity of SOD changed. A significant increase in the intracellular SOD1 concentration in plasma of AP patients proves the important role of this isoenzyme in the neutralization of oxidative stress induced by impaired Cu and Zn homeostasis. The presence of increased concentration of SOD2 in erythrocytes of healthy subjects and AP patients confirms the important function of this isoenzyme in the antioxidative defense.

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Section: Discussionmentioning

confidence: 99%

Changes in the Activity and Concentration of Superoxide Dismutase Isoenzymes (Cu/Zn SOD, MnSOD) in the Blood of Healthy Subjects and Patients with Acute Pancreatitis

et al. 2020

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“…Exendin-4, a glucagon-like peptide-1 receptor agonist, significantly induces the expression of SOD3 in human retinal microvascular ECs (HRECs) by decreasing HDAC activity and enhancing the status of histone H3 acetylation at the SOD3 proximal promoter and ( Yasuda et al, 2016 ). Moreover, Caffeic acid phenethyl ester (CAPE) increases the transcription of SOD3 through HDAC1-regulated histone acetylation in HRECs ( Ohashi et al, 2017 ). Except for SOD3, SOD2 (Superoxide Dismutase 2, also called MnSOD) is also regulated by histone acetylation.…”

Section: Role Of Endothelial Histone Acetylation In Multiple Physiological Processesmentioning

confidence: 99%

The Role of Histone Protein Acetylation in Regulating Endothelial Function

Fang

Wang

Sun

et al. 2021

Front. Cell Dev. Biol.

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Endothelial cell (EC), consisting of the innermost cellular layer of all types of vessels, is not only a barrier composer but also performing multiple functions in physiological processes. It actively controls the vascular tone and the extravasation of water, solutes, and macromolecules; modulates circulating immune cells as well as platelet and leukocyte recruitment/adhesion and activation. In addition, EC also tightly keeps coagulation/fibrinolysis balance and plays a major role in angiogenesis. Therefore, endothelial dysfunction contributes to the pathogenesis of many diseases. Growing pieces of evidence suggest that histone protein acetylation, an epigenetic mark, is altered in ECs under different conditions, and the acetylation status change at different lysine sites on histone protein plays a key role in endothelial dysfunction and involved in hyperglycemia, hypertension, inflammatory disease, cancer and so on. In this review, we highlight the importance of histone acetylation in regulating endothelial functions and discuss the roles of histone acetylation across the transcriptional unit of protein-coding genes in ECs under different disease-related pathophysiological processes. Since histone acetylation changes are conserved and reversible, the knowledge of histone acetylation in endothelial function regulation could provide insights to develop epigenetic interventions in preventing or treating endothelial dysfunction-related diseases.

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“…Caffeic acid was also reported to attenuate high glucose concentration‐induced redox changes in other cellular types including human retinal endothelial cells and human umbilical vein endothelial cells through regulation of SOD and Nrf2. [ 46,47 ] The antioxidant capacity of A. borbonica polyphenols and caffeic acid may also result from their ability to directly scavenge ROS, as we previously showed using in vitro free radical‐scavenging assays. [ 21,22 ] Moreover, polyphenols such as caffeic acid are able to improve mitochondrial metabolism during oxidative stress.…”

Section: Discussionmentioning

confidence: 99%

Protective Effects of Antioxidant Polyphenols against Hyperglycemia‐Mediated Alterations in Cerebral Endothelial Cells and a Mouse Stroke Model

Arcambal

Taïlé

Couret

et al. 2020

Molecular Nutrition Food Res

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Scope Hyperglycemia alters cerebral endothelial cell and blood‐brain barrier functions, aggravating cerebrovascular complications such as stroke during diabetes. Redox and inflammatory changes play a causal role. This study evaluates polyphenol protective effects in cerebral endothelial cells and a mouse stroke model during hyperglycemia. Methods and results Murine bEnd.3 cerebral endothelial cells and a mouse stroke model are exposed to a characterized, polyphenol‐rich extract of Antirhea borbonica or its predominant constituent caffeic acid, during hyperglycemia. Polyphenol effects on redox, inflammatory and vasoactive markers, infarct volume, and hemorrhagic transformation are determined. In vitro, polyphenols improve reactive oxygen species levels, Cu/Zn superoxide dismutase activity, and both NAPDH oxidase 4 and nuclear factor erythroid 2‐related factor 2 (Nrf2) gene expression deregulated by high glucose. Polyphenols reduce Nrf2 nuclear translocation and counteract nuclear factor‐ĸappa B activation, interleukin‐6 secretion, and the altered production of vasoactive markers mediated by high glucose. In vivo, polyphenols reduce cerebral infarct volume and hemorrhagic transformation aggravated by hyperglycemia. Polyphenols attenuate redox changes, increase vascular endothelial‐Cadherin production, and decrease neuro‐inflammation in the infarcted hemisphere. Conclusion Polyphenols protect against hyperglycemia‐mediated alterations in cerebral endothelial cells and a mouse stroke model. It is relevant to assess polyphenol benefits to improve cerebrovascular damages during diabetes.

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CAPE increases the expression of SOD3 through epigenetics in human retinal endothelial cells

Cited by 12 publications

References 58 publications

Changes in the Activity and Concentration of Superoxide Dismutase Isoenzymes (Cu/Zn SOD, MnSOD) in the Blood of Healthy Subjects and Patients with Acute Pancreatitis

Changes in the Activity and Concentration of Superoxide Dismutase Isoenzymes (Cu/Zn SOD, MnSOD) in the Blood of Healthy Subjects and Patients with Acute Pancreatitis

The Role of Histone Protein Acetylation in Regulating Endothelial Function

Protective Effects of Antioxidant Polyphenols against Hyperglycemia‐Mediated Alterations in Cerebral Endothelial Cells and a Mouse Stroke Model

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