Capacity of two Staphylococcus aureus strains with different adaptation genotypes to persist and induce damage in bovine mammary epithelial cells and to activate macrophages

Sacco, Sofía Clara; Velazquez, Natalia Soledad; Renna, María Sol; Beccaría, Camila; Baravalle, Celina; Pereyra, Elizabet Amanda Lorena; Monecke, Stefan; Calvinho, Luis Fernando; Dallard, Bibiana Elisabet

doi:10.1016/j.micpath.2020.104017

Cited by 11 publications

(11 citation statements)

References 52 publications

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“…As a result, both adhesion rate and invasion rate decreased. This was in general consistent with the results of other studies where the number of bacteria surviving inside the cells decreased over time (28). Furthermore, the adhesion and the invasion rate of MSSA-infected MAC-T cells were significantly lower than that of MRSA-infected MAC-T, and the invasion rate of MSSA-infected MAC-T cells did not vary significantly over time.…”

Section: Discussionsupporting

confidence: 92%

“…This study found that both MRSA and MSSA could adhere to and invade MAC-T cells at different degrees, with MRSA had a stronger ability to adhere to and invade MAC-T cells. It has been demonstrated that various strains have different abilities to adhere to and invade cells (28). In this study, the adhesion and invasion rates of MRSA-infected MAC-T cells gradually increased with time, peaking at 8 h and then declining.…”

Section: Discussionmentioning

confidence: 56%

“…The colony forming units per milliliter (CFU/ml) of MRSA and MSSA in MAC-T cells were determined by standard colony counting techniques. Each strain and condition were tested in three independent experiments (28).…”

Section: Adherence and Invasion Assays In Mac-t Cellsmentioning

confidence: 99%

“…Finally, these treated MAC-T cells were rapidly detected by flow cytometry (BD LSRFortessa TM Cell Analyzer, 2010 BD, USA). The data were analysis by BD FACSDiva Software (28).…”

Section: The Mac-t Cells Apoptosis Assaysmentioning

confidence: 99%

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Effect of Two Different Drug-Resistant Staphylococcus aureus Strains on the Physiological Properties of MAC-T Cells and Their Transcriptome Analysis

Yan

Yang

et al. 2022

Front. Vet. Sci.

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Staphylococcus aureus (S. aureus) is one of the main pathogens causing mastitis in dairy cows. The current work mainly focuses on the pathway of apoptosis induction in MAC-T cells caused by S. aureus infection or other factors. However, the physiological characteristics of S. aureus infected MAC-T cells and the resulting mRNA expression profile remain unknown particularly in the case of diverse drug resistant strains. Methicillin-resistant S. aureus (MRSA) and methicillin-susceptible S. aureus (MSSA) strains were used to infect MAC-T cells to investigate this issue. The adhesion, invasion and apoptosis ability of MRSA-infected group and MSSA-infected group was assessed over time (2, 4, 6, 8, and 12 h). After 8 h, the RNA sequencing was conducted on the MRSA-infected and the MSSA-infected with uninfected MAC-T cells as controls. The results showed that the adhesion and invasion ability of MRSA-infected and MSSA-infected to MAC-T cells increased and then decreased with infection time, peaking at 8 h. The adhesion and invasion rates of the MSSA-infected were substantially lower than those of the MRSA-infected, and the invasion rate of the MSSA-infected group was nearly non-existent. Then the apoptosis rate of MAC-T cells increased as the infection time increased. The transcriptome analysis revealed 549 differentially expressed mRNAs and 390 differentially expressed mRNAs in MRSA-infected and MSSA-infected MAC-T cells, respectively, compared to the uninfected MAC-T cells. According to GO analysis, these differentially expressed genes were involved in immune response, inflammation, apoptosis, and other processes. The Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis indicated the following pathways were linked to adhesion, invasion inflammation and apoptosis, including AMPK, FOXO, HIF-1, IL-17, JAK-STAT, MAPK, mTOR, NF-κB, p53, PI3K-Akt, TNF, Toll-like receptor, Rap1, RAS, prion disease, the bacterial invasion of epithelial cells pathway. We found 86 DEGs from 41 KEGG-enriched pathways associated with adhesion, invasion, apoptosis, and inflammation, all of which were implicated in MAC-T cells resistance to MRSA and MSSA infection. This study offers helpful data toward understanding the effect of different drug-resistant S. aureus on dairy cow mammary epithelial cells and aid in the prevention of mastitis in the dairy industry.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 56%

Section: Adherence and Invasion Assays In Mac-t Cellsmentioning

confidence: 99%

“…Finally, these treated MAC-T cells were rapidly detected by flow cytometry (BD LSRFortessa TM Cell Analyzer, 2010 BD, USA). The data were analysis by BD FACSDiva Software (28).…”

Section: The Mac-t Cells Apoptosis Assaysmentioning

confidence: 99%

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Effect of Two Different Drug-Resistant Staphylococcus aureus Strains on the Physiological Properties of MAC-T Cells and Their Transcriptome Analysis

Yan

Yang

et al. 2022

Front. Vet. Sci.

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“…Biofilm is one of the major virulence factors of S. aureus responsible for persistent infections and treatment difficulties. The biofilm formation could be considered as survival strategy against host immunity and applied therapy, during the establishment of the infection (Sacco et al, 2020). Therefore, because this pathogen is also associated with infections that arise from medical device contaminations, prevention of cell attachment and biofilm formation represents a good preventative strategy (Suresh et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

Elucidating the antibiofilm activity of Frangula emodin against Staphylococcus aureus biofilms

Đukanović

Ganić

Lončarević

et al. 2022

Journal of Applied Microbiology

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Aims: Because the Staphylococcus aureus is one of the most well-known pathogens associated with medical devices and nosocomial infections, the aim of the study was to examine antibiofilm potential of emodin against it.Methods and Results: Antibacterial activity was examined through microdilution assay. Antibiofilm testing included crystal violet staining of biofilm biomass and morphology analysis by Atomic force microscopy (AFM). Furthermore, aerobic respiration was monitored using the Micro-Oxymax respirometer. For investigation of gene expression qRT-PCR was performed. Emodin demonstrated strong antibacterial activity and ability to inhibit biofilm formation of all tested strains. The effect on preformed biofilms was spotted in few strains. AFM revealed that emodin affects biofilm structure and roughness. Monitoring of respiration under emodin treatment in planktonic and biofilm form revealed that emodin influenced aerobic respiration.Moreover, qRT-PCR showed that emodin modulates expression of icaA, icaD, srrA and srrB genes, as well as RNAIII, and that this activity was strain-specific. Conclusion:The results obtained in this study indicate the novel antibiofilm activity of emodin and its multiple pathways of action. Significance and Impact of Study:This is the first study that examined pathways through which emodin expressed its antibiofilm activity.

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Differential immune response to two Staphylococcus aureus strains with distinct adaptation genotypes after experimental intramammary infection of dairy cows

Engler

Renna

Beccaría

et al. 2022

Microbial Pathogenesis

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Capacity of two Staphylococcus aureus strains with different adaptation genotypes to persist and induce damage in bovine mammary epithelial cells and to activate macrophages

Cited by 11 publications

References 52 publications

Effect of Two Different Drug-Resistant Staphylococcus aureus Strains on the Physiological Properties of MAC-T Cells and Their Transcriptome Analysis

Effect of Two Different Drug-Resistant Staphylococcus aureus Strains on the Physiological Properties of MAC-T Cells and Their Transcriptome Analysis

Elucidating the antibiofilm activity of Frangula emodin against Staphylococcus aureus biofilms

Differential immune response to two Staphylococcus aureus strains with distinct adaptation genotypes after experimental intramammary infection of dairy cows

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