Capacity of soybean carbohydrate metabolism in Leuconostoc mesenteroides, Lactococcus lactis and Streptococcus thermophilus

Evaluation of the fermentation potential of lactic acid bacteria isolated from herbs, fruits and vegetables as starter cultures in nut-based milk alternatives

“…lactis sacA1 indicates that the gene sacA is essential in the sucrose metabolic pathway. This finding is consistent with that of Yu et al (2021), who found that one Lc. lactis strain with the PTS pathway, but lacking sacA gene, was unable to grow and produce acid in a modified medium with sucrose as the sole carbon source.…”

“…Different from dairy-isolated LAB, plant-derived LAB are commonly found to be able to utilise sucrose, since plants are regarded as the primary habitat for LAB where sucrose is abundant (Golomb and Marco, 2015). Current attempts to develop new fermented plant-based yoghurts are utilising dairy-derived LAB as starter cultures, primarily S. thermophilus (Jan et al, 2022;Yu et al, 2021;Zhao et al, 2021). We also found that a yoghurt strain of S. thermophilus was able to acidify sucrose-rich almond PBMA, albeit slower and not as low a final pH as a plant-derived Lc.…”

Corrigendum to: Leuconostoc performance in soy-based fermentations – Survival, acidification, sugar metabolism, and flavor comparisons [Food Microbiol. 115 (2023) 104337]

“…For NFICC28 and NFICC99 a gene encoding a fructokinase was found in proximity to a mannitol dehydrogenase gene (data not shown), which might indicate that the control of these functions is more co-regulated for these strains. The presence of the sucrose cluster, as well as the composition of genes, is in accordance with other studies (Yu et al, 2021) and with the observed phenotypes.…”

“…mesenteroides strains NFICC311, NFICC319, NFICC320, and NFICC321 is interesting, as they all contained the same cluster for RFO utilization. Others, who have investigated the utilization of soybean carbohydrates (Yu et al, 2021), found that strains of Ln. mesenteroides with interpositioned lacI between lacS and galA had a much lower α-galactosidase activity compared to strains lacking the lacI gene.…”

“…Fig. 5 shows the presence of an α-galactosidase gene cluster, harboring a raffinose permease (lacS), transcriptional regulator (lacI), an α-galactosidase (galA), a galactokinase (galK), and a galactose-1phosphate uridylyltransferase (galT) (Gänzle and Follador, 2012;Harlé et al, 2020;Yu et al, 2021). All strains, except NFICC28 harbored the necessary genes for successful RFO internalization and hydrolyzation.…”