The protective effect of lactose on the bile salt stress response of Streptococcus thermophilus is strain dependent

Pan, Yuqi; Yu, Peng; Jiang, Yang; Liu, Xiaoming; Zhao, Jianxin; Zhang, Hao; Chen, Wei

doi:10.1016/j.fbio.2023.102560

Cited by 2 publications

(1 citation statement)

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“…A previous study has shown that lactose consumption contributed to the rapid and high level of S. thermophilus LMD9 colonization in the fecal samples of gnotobiotic rats [14]. Our previous research demonstrated that lactose could protect the bile salt stress response of S. thermophilus in a strain-independent manner in vitro [15]. Furthermore, we have investigated the effect on the gut microbiota and host metabolism in C57BL/6J mice by multiple S. thermophilus strains based on inter-strain differences.…”

Section: Introductionmentioning

confidence: 99%

Influence of Lactose Supplementation on Regulation of Streptococcus thermophilus on Gut Microbiota

Yu,

Pan,

Pei

et al. 2023

Nutrients

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It has been found that Streptococcus thermophilus (S. thermophilus) influenced the gut microbiota and host metabolism with strain specificity in C57BL/6J mice in the previous study, though it remains unclear whether lactose as a dietary factor associated with dairy consumption is involved as the mediator in the interaction. In the present study, integrated analysis of 16S rRNA gene sequencing and untargeted metabolomics by liquid chromatography–mass spectrometry of fecal samples in C57BL/6J mice was applied to evaluate the effect of lactose on the regulation of gut microbiota by two S. thermophilus strains (4M6 and DYNDL13-4). The results showed that the influence of lactose supplementation on gut microbiota induced by S. thermophilus ingestion was strain-specific. Although two S. thermophilus strains ingestion introduced similar perturbations in the fecal microbiota and gut microbial metabolism, the regulation of DYNDL13-4 on the gut microbiota and metabolism was more affected by lactose than 4M6. More specifically, lactose and 4M6 supplementation mainly enriched pathways of d-glutamine and d-glutamate metabolism, alanine, aspartate, and glutamate metabolism, and tryptophan and phenylalanine metabolism in the gut, whereas 4M6 only enriched tryptophan and phenylalanine metabolism. DYNDL13-4-L (DYNDL13-4 with lactose) had significant effects on sulfur, taurine, and hypotaurine metabolism in the gut and on phenylalanine, tyrosine, tryptophan biosynthesis, and linoleic acid metabolism in serum relative to the DYNDL13-4. Our study demonstrated the strain-specific effect of lactose and S. thermophilus supplementation on gut microbiota and host metabolism. However, considering the complexity of the gut microbiota, further research is necessary to provide insights to facilitate the design of personalized fermented milk products as a dietary therapeutic strategy for improving host health.

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Section: Introductionmentioning

confidence: 99%