Capacitance method to determine the microbiological quality of raw shrimp (Penaeus setiferus)

Metcalfe, Allie; Marshall, Douglas L.

doi:10.1016/j.fm.2003.09.002

Cited by 7 publications

(3 citation statements)

References 15 publications

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“…A rapid detection method, able to yield same-day results, was proposed for real-time detection of bacteria in drinking water [4] . Metcalfe and Marshall [5] suggested that capacitance detection method, acted as a rapid alternative microbial counts method, had a good detection accuracy and short detection time (4-9 hours). Furthermore, near-infrared chemical imaging was studied for microbial identification test cards [6] .…”

Section: Introductionmentioning

confidence: 99%

A close to Real-Time Intelligent System for Enumerating Total Viable Bacteria Based on Pattern Recognition Technology

Ding

Yin

2015

AMM

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In this paper, a novel close to real-time artificial intelligent system for enumerating Total Viable Bacteria (TVB) in drinking water was developed by using pattern recognition and machine vision technology. In order to identify the viable bacteria accurately, four shape features including circularity ratio, eccentricity, rectangularity, and compact degree, and four color features (GRsd, BRsd, HRsd, SRsd) of the stained viable bacteria image were extracted. An optimal artificial neural network was used as the bacterial recognition classifier, whose inputs were the extracted feature parameters and output was bacteria signal or non-bacteria signal. By using this intelligent system, TVB counts in each sample can be enumerated within 1 h, but the traditional Aerobic Plate Count (APC) method will take us 48 h. The comparative test also indicated that the counting results by two methods are closely correlated (R2=0.9942). This close to real-time accurate information may contribute to melioration and instauration of drinking water safety systems and risk management for TVB.

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Section: Introductionmentioning

confidence: 99%

A close to Real-Time Intelligent System for Enumerating Total Viable Bacteria Based on Pattern Recognition Technology

Ding

Yin

2015

AMM

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“…In the impedance technique, by means of an electrode system in the glass tubes, impedance values can be recorded over time for a pre-selected incubation period (7). The concentration of ions generated by the bacteria reach a magnitude at which a measurable increase in conductivity can be detected.…”

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confidence: 99%

“…Therefore, bacterial counts can be estimated by measurement of the IDT. Finally, at the termination of the incubation periods (24 and 48 hours), the printouts of the impedance patterns and the IDTs expressed in hours for each test cell are produced (2,7). Usually, IDT is not visible until the number of bacteria reaches approximately 10 6 -10 7 CFU/mL.…”

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confidence: 99%

Impediometric Technique as a Rapid Estimation to Detect Pathogenic Bacteria in Quality Control

Ekhtelat

2016

Jundishapur J Nat Pharm Prod

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It is of interest for drug-and food-producing industries to acquire information about the microbiological quality of their products as early as possible. Conventional microbiological methods for the detection and identification of pathogens include microbiological culturing and isolation of the pathogens, followed by approbation with biochemical and serological tests. These methods are timeconsuming and labor-intensive, not suitable for a rapid response for high-risk pathogens. As a result, several instruments have been developed for use much earlier, including nucleic acid-based tests, serological methods, and some automated instrumental diagnostic assays (1).The impedance measurement is used as a rapid method to detect microbial growth. It is based on a measurement of the changes in electrical conductance of the culture media that are produced by microbial metabolism. Impedance is the resistance to flow of an alternating current through a conducting material. The examined microorganisms are cultivated in impedance glass tubes (cells) in fluid growth medium, with highly uncharged molecules that are fitted with measuring electrodes. The culture medium is metabolized by microbial activity, and the impedance changes and decreases because of the production of small charged molecules by the microorganism's metabolites (2). The impedance measurement is a complex entity composed of a combination of a conductive element and a capacitive element. Therefore, in monitoring microbial growth, conductance measurements can indicate changes taking place in the bulk solution or medium (3). The specific growth medium used in the impediometric technique supports the target bacteria, and must be formulated to provide optimal impedance signals to provide specificity to the impedance microbiological method (2).Impedance measurements are based on the bacterial growth and metabolic processes that produce electrically measurable changes in the growth medium. These changes are due to the metabolism of high-molecularweight nutrients into smaller charged ionic molecules, which increase the electricity conductivity of the specific medium. Therefore, the impedance technique distinguishes viable and dead pathogens rapidly, within 24 hours (4). Variation in electrical conductivity is related to the change in bacterial number, and thus bacterial growth can be measured. Accordingly, impedance measurements are considered for quality control in the food, drug, and hygiene-product industries, especially for the identification and enumeration of indicator microorganisms (2, 5).In glass tubes, the electrical change is recorded using a pair of electrodes immersed in the growth medium. The impedance measurement can be done by two methods: direct or indirect. In the direct method, a pair of metal electrodes is submerged in the medium that is inoculated with the testing sample. The electrical change caused by the release of ionic metabolites from live cells in the medium is monitored over time (6). In the indirect method, the electrodes are immersed in ...

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Microbiological Examination of Seafood

Boziaris

Parlapani

2013

Seafood Processing

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Capacitance method to determine the microbiological quality of raw shrimp (Penaeus setiferus)

Cited by 7 publications

References 15 publications

A close to Real-Time Intelligent System for Enumerating Total Viable Bacteria Based on Pattern Recognition Technology

A close to Real-Time Intelligent System for Enumerating Total Viable Bacteria Based on Pattern Recognition Technology

Impediometric Technique as a Rapid Estimation to Detect Pathogenic Bacteria in Quality Control

Microbiological Examination of Seafood

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