Capability Enhancement of Fumaric Acid Production by Rhizopus arrhizus Through Carbon-Nitrogen Sources Coordination

Xing, Hao; Liu, Huan; Zhang, Yakun; Yu, Yue; Xiao, Qi; Deng, Li; Wang, Fang

doi:10.1007/s12010-020-03461-0

Cited by 3 publications

(3 citation statements)

References 21 publications

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“…The mycelia were clearly observed and immobilized on the carriers. The biomass reached 9.89 g/L after spore inoculum inoculated for 24 h, which was similar to the previous results (Xing et al 2020). This result indicated that the growth of the strain was not impacted by the high-concentration glucose, which is necessary for fumaric acid production using one-step fermentation.…”

Section: Development Of the One-step Fermentation Methodssupporting

confidence: 89%

“…These data illustrated that fumaric acid production could be significantly promoted through two-stage regulation of agitation speed in the one-step fermentation process. The production of fumaric acid was higher than that obtained from two-step fermentation under the same conditions (Xing et al 2020). In two-step fermentation, the seed culture stage is usually 24 to 48 h, and then Rhizopus cells are transferred into the fermentation medium for fumaric acid production for 96 to 144 h (Kowalczyk et al 2018;Swart et al 2020).…”

Section: Two-stage Regulation Of Agitation Speed In the Stirred-tank Reactormentioning

confidence: 92%

“…In traditional two-step fermentation, the nitrogen source is the critical factor for fumaric acid production (Xing et al 2020). Therefore, the level of nitrogen source peptone was optimized for one-step fermentation.…”

Section: Optimization Of Nitrogen Source Level In the One-step Fermentationmentioning

confidence: 99%

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Applications of one-Step environmentally-friendly fermentation method to produce fumaric acid with immobilized Rhizopus arrhizus in stirred-tank reactor

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et al. 2021

BioRes

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The traditional two-step cultivation mode for fungal cultivation is commonly divided into the seed stage (usually 24 to 48 h) and the production stage (usually 96 to 144 h). The use of two stages prolongs the total production cycle and generates excess wastewater. In this work, an efficient and environmentally friendly one-step fermentation method was applied for the production of fumaric acid by immobilized Rhizopus arrhizus in a stirred-tank reactor. The nitrogen source content and the agitation speed of the reactor were optimized as the two critical factors in the one-step fermentation process; the fumaric acid production of 51 g/L with a yield of 0.42 g/g glucose was comparable with the product from the traditional two-step fermentation process. Furthermore, the total production cycle was shortened to 96 h, and the amount of wastewater was reduced due to the avoidance of the seed culture process. Thus, utilization of the one-step fermentation method to produce fumaric acid was shown to be preferable feasibility and environmental-friendliness. This is a promising method for industrial production of fumaric acid and other high-value biochemicals by fungus.

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Section: Development Of the One-step Fermentation Methodssupporting

confidence: 89%