Recent advances and perspectives on production of value-added organic acids through metabolic engineering

Liu, Huan; Jin, Yuhan; Zhang, Renwei; Ning, Yuchen; Yu, Yue; Xu, Peng; Deng, Li; Wang, Fang

doi:10.1016/j.biotechadv.2022.108076

Cited by 26 publications

(7 citation statements)

References 176 publications

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“…Microbial production of cc MA from glucose has been reported in bacteria that either lack or have a limited ability to metabolize the mixed aromatics that are abundant in plant biomass ( 2 , 52 ). Many efforts to produce cc MA from aromatics have been limited by the accumulation of pathway intermediates ( 6 , 20 , 22 , 23 ).…”

Section: Discussionmentioning

confidence: 99%

Engineering Novosphingobium aromaticivorans to produce cis,cis -muconic acid from biomass aromatics

Vilbert,

Kontur,

Gille

et al. 2024

Appl Environ Microbiol

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The platform chemical cis,cis- muconic acid ( cc MA) provides facile access to a number of monomers used in the synthesis of commercial plastics. It is also a metabolic intermediate in the β-ketoadipic acid pathway of many bacteria and, therefore, a current target for microbial production from abundant renewable resources via metabolic engineering. This study investigates Novosphingobium aromaticivorans DSM12444 as a chassis for the production of cc MA from biomass aromatics. The N. aromaticivorans genome predicts that it encodes a previously uncharacterized protocatechuic acid (PCA) decarboxylase and a catechol 1,2-dioxygenase, which would be necessary for the conversion of aromatic metabolic intermediates to cc MA. This study confirmed the activity of these two enzymes in vitro and compared their activity to ones that have been previously characterized and used in cc MA production. From these results, we generated one strain that is completely derived from native genes and a second that contains genes previously used in microbial engineering synthesis of this compound. Both of these strains exhibited stoichiometric production of cc MA from PCA and produced greater than 100% yield of cc MA from the aromatic monomers that were identified in liquor derived from alkaline pretreated biomass. Our results show that a strain completely derived from native genes and one containing homologs from other hosts are both capable of stoichiometric production of cc MA from biomass aromatics. Overall, this work combines previously unknown aspects of aromatic metabolism in N. aromaticivorans and the genetic tractability of this organism to generate strains that produce cc MA from deconstructed biomass. IMPORTANCE The production of commodity chemicals from renewable resources is an important goal toward increasing the environmental and economic sustainability of industrial processes. The aromatics in plant biomass are an underutilized and abundant renewable resource for the production of valuable chemicals. However, due to the chemical composition of plant biomass, many deconstruction methods generate a heterogeneous mixture of aromatics, thus making it difficult to extract valuable chemicals using current methods. Therefore, recent efforts have focused on harnessing the pathways of microorganisms to convert a diverse set of aromatics into a single product. Novosphingobium aromaticivorans DSM12444 has the native ability to metabolize a wide range of aromatics and, thus, is a potential chassis for conversion of these abundant compounds to commodity chemicals. This study reports on new features of N. aromaticivorans that can be used to produce the commodity chemical cis,cis -muconic acid from renewable and abundant biomass aromatics.

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Section: Discussionmentioning

confidence: 99%

Engineering Novosphingobium aromaticivorans to produce cis,cis -muconic acid from biomass aromatics

Vilbert,

Kontur,

Gille

et al. 2024

Appl Environ Microbiol

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“…In such a scenario, the excess carbon from metabolic pathways like glycolysis and gluconeogenesis could surpass the capacity of downstream pathways such as the citric acid cycle. Consequently, a metabolic diversion will occur, resulting in the production of these compounds in addition to lipid metabolism [69,70]. As illustrated in Figure 8a, they are involved in furfural degradation metabolism, ultimately contributing to the citric acid cycle (citrate cycle).…”

Section: Comparison Of Organic Acid Production Using Glucose As the C...mentioning

confidence: 99%

Determining the Metabolic Processes of Metal-Tolerant Fungi Isolated from Mine Tailings for Bioleaching

Nkuna,

Matambo

2024

Minerals

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This study examined the metal tolerance and organic acid-producing capabilities of fungal isolates from South African tailings to assess their potential for future bioleaching applications. Four isolates were chosen for additional examination based on their capacity to generate organic acids and tolerance to metals. In terms of tolerance to Al, Zn, Ni, and Cr, these four isolates—Trichoderma, Talaromyces, Penicillium_3, and Penicillium_6—displayed varying degrees of resistance, with Trichoderma displaying a better metal tolerance index. The growth rates under metal stress varied among the isolates, with Trichoderma displaying the highest growth rates. In high-performance liquid chromatography results, citric acid emerged as the primary organic acid produced by the four isolates, with Trichoderma achieving the highest yield in the shortest timeframe. Gas chromatography–mass spectrometry results showed that the citric acid cycle is one of the main pathways for organic acid production, though other pathways related to lipid biosynthesis and carbohydrate metabolism also play significant roles. Three compounds involved in furfural breakdown were abundant. Using KEGG, a link between these compounds and the citric acid cycle was established, where their breakdown generates an intermediate of the citric acid cycle.

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“…Bioproduction of chemicals by microbial cell factories is considered a promising route to address the growing demand for renewable chemicals and alleviate the dependence upon petrochemical resources . In recent years, with the development of genetic engineering tools, there is growing interest in the production of renewable products in microbes through metabolic engineering strategies, and a diverse range of biochemicals has been produced via engineered microbial cell factories . Polyketides as a large group of secondary metabolites have become a platform biochemical due to their wide range of bioactivities in terms of antibacterial, anticancer, anticholesterol, and anti-inflammatory properties …”

Section: Introductionmentioning

confidence: 99%

“…2 In recent years, with the development of genetic engineering tools, there is growing interest in the production of renewable products in microbes through metabolic engineering strategies, and a diverse range of biochemicals has been produced via engineered microbial cell factories. 3 Polyketides as a large group of secondary metabolites have become a platform biochemical due to their wide range of bioactivities in terms of antibacterial, anticancer, anticholesterol, and anti-inflammatory properties. 4 Triacetic acid lactone as a small molecule polyketide was recognized as an important platform compound that could be converted into a range of commercial products and a variety of value-added fine chemicals, such as sorbic acid, acetylacetone, pogostone, kavalactone, and so on.…”

Section: Introductionmentioning

confidence: 99%

Efficient Production of Triacetic Acid Lactone from Lignocellulose Hydrolysate by Metabolically Engineered Yarrowia lipolytica

Liu,

Huang,

Liu

et al. 2023

J. Agric. Food Chem.

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Recent advances and perspectives on production of value-added organic acids through metabolic engineering

Cited by 26 publications

References 176 publications

Engineering Novosphingobium aromaticivorans to produce cis,cis -muconic acid from biomass aromatics

Engineering Novosphingobium aromaticivorans to produce cis,cis -muconic acid from biomass aromatics

Determining the Metabolic Processes of Metal-Tolerant Fungi Isolated from Mine Tailings for Bioleaching

Efficient Production of Triacetic Acid Lactone from Lignocellulose Hydrolysate by Metabolically Engineered Yarrowia lipolytica

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