Canine leishmaniasis in south-east of France: Screening of Leishmania infantum antibodies (western blotting, ELISA) and parasitaemia levels by PCR quantification

Aoun, Olivier; Mary, Charles; Roqueplo, Cédric; Marié, Jean-Lou; Terrier, Olivier; Levieuge, Aurélie; Davoust, Bernard

doi:10.1016/j.vetpar.2009.08.006

Cited by 28 publications

(20 citation statements)

References 19 publications

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“…PCR has been successfully used to detect Leishmania DNA in several biological materials such as blood, bone marrow, skin, conjunctival samples and cerebrospinal fluid (LACHAUD et al, 2002;AOUN et al, 2009). Other advantages of PCR include the possibility of using small amounts of different clinical samples and detection of low concentrations of parasites (MELO, 2004).…”

Section: Introductionmentioning

confidence: 99%

Design of primer pairs for species-specific diagnosis of Leishmania (Leishmania) infantum chagasi using PCR

Neto

Duarte

Costa

et al. 2012

Rev. Bras. Parasitol. Vet.

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The objective of this study was to design and evaluate new primers for species-specific detection of L. infantum chagasi using PCR. Two combinations of primer pairs were established with the aim of obtaining specific amplification products from the L. infantum chagasi 18S rRNA gene. The combinations of the primer pairs and the respective sizes of the PCR products, based on the U422465 GenBank reference sequence of L. infantum chagasi, were: LCS1/LCS3 (259 bp) and LCS2/LCS3 (820 bp). It was concluded that the new PCR assays optimized using the primer pairs LCS1/LCS3 and LCS2/LCS3 were effective for specific detection of L. infantum chagasi, with analytical sensitivity to detect 1 pg/µL of DNA.

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Section: Introductionmentioning

confidence: 99%

Design of primer pairs for species-specific diagnosis of Leishmania (Leishmania) infantum chagasi using PCR

Neto

Duarte

Costa

et al. 2012

Rev. Bras. Parasitol. Vet.

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“…In In a previous study, 66.6% of blood samples of military working dogs of Var were PCR-positive for L. infantum. These dogs had a low level of parasitemia (Aoun et al 2009). The role of red foxes in the epidemiologic cycle of leishmaniosis should be further investigated.…”

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confidence: 97%

“…Domestic dogs (Canis lupus familiaris) are considered the main reservoir of the parasite (Aoun et al 2009). The potential role of wild carnivores, including Canidae, as sentinels or reservoirs of L. infantum is debated.…”

mentioning

confidence: 99%

“…For maximum yield, we performed overnight breakdown with proteinase K in animal tissue lysis buffer before purification. DNA was eluted in 100 mL of distilled water and stored at 280 C. We used a real-time quantitative PCR for the detection and quantification of L. infantum DNA as previously described (Mary et al 2004;Mary et al 2006;Aoun et al 2009). Assays were performed in 25-mL final volume using 1 mL of sample DNA.…”

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confidence: 99%

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Detection ofLeishmaniain Red Foxes (Vulpes vulpes) from Southeastern France Using Real-time Quantitative PCR

Davoust

Mary

Marié³

2014

Journal of Wildlife Diseases

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“…However, lower prevalence (2.1% and 2.5%) was also reported in the northern Italy (Maroli et al, 2008;Baldelli et al, 2011). In France, a study performed using the PCR and western-blotting techniques indicated that a high number of dogs in southern France (Berrahal et al, 1996) and up to 50 % in southeastern France have been exposed to Leishmania (Aoun et al, 2009). In another study in France, 79.8% and 29.6% positivity were found in 263 dogs by PCR and serology, BILGIN Z., TURAN N., YILMAZ H., FERROGLIO E., TUZER E.…”

Section: Discussionmentioning

confidence: 98%

Prevalence of leishmaniosis in dogs in Istanbul,Turkey determined by using PCR

Bilgin

Turan

Yılmaz

et al. 2018

J Hellenic Vet Med Soc

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ABSTRACT. Leishmania infantum is widespread in Mediterranean countries including Turkey and can cause a serious disease in both humans and dogs. Dogs are considered to be its main reservoirs. The current epidemiological study was carried out in Istanbul for detection of leishmaniosis among dogs. A total of 246 dogs were included in the study. Twenty one (8.54%) blood samples were found to be positive based on PCR diagnosis, using primers specific for the kinetoplast DNA of Leishmania. Infection rate was 6.51% in 169 dogs living in shelters and 12.99% in 77 client-owned dogs. The rate was significantly (p<0.001) higher in the dogs (37.93%) presenting one or more clinical symptoms which may be attributable to leishmaniosis, than in the asymptomatic dogs (4.61%). Eleven (52.4%) of 21 PCR positive dogs presented clinical symptoms whereas the rest of the dogs (47.62%) were asymptomatic. The major clinical sign in PCR positive animals was dermatological problems. Amongst PCR positive dogs, skin lesions were present in 11, weight loss in 2, lymphadenopathy in 2, epistaxis in 2 and lethargy in 2 dogs.

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Canine leishmaniasis in south-east of France: Screening of Leishmania infantum antibodies (western blotting, ELISA) and parasitaemia levels by PCR quantification

Cited by 28 publications

References 19 publications

Design of primer pairs for species-specific diagnosis of Leishmania (Leishmania) infantum chagasi using PCR

Design of primer pairs for species-specific diagnosis of Leishmania (Leishmania) infantum chagasi using PCR

Detection ofLeishmaniain Red Foxes (Vulpes vulpes) from Southeastern France Using Real-time Quantitative PCR

Prevalence of leishmaniosis in dogs in Istanbul,Turkey determined by using PCR

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