Canine ehrlichiosis: clinical, hematological, serological and molecular aspects

Nakaghi, Andrea Cristina Higa; Machado, Rosangela Zacarias; Costa, Mirela Tinucci; André, Marcos Rogério; Baldani, Cristiane Divan

doi:10.1590/s0103-84782008000300027

Cited by 97 publications

(143 citation statements)

References 15 publications

(11 reference statements)

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“…Thus, the hematological findings were nonspecific, with both anemia and thrombocytopenia being identified in some dogs, but with most values of the erythrogram and the platelet count falling within the normal range for canines. Similarly, 30 dogs examined at the Veterinary Teaching Hospital of the São Paulo State University (UNESP), in Jaboticabal city, Brazil showed no association between the hematological signs (trombocytopenia and anemia; n= 13) and serological (IFAT) positive (n= 7) or negative (n= 6) results (30) . In 70 dogs suspected of E. canis infection attended at the Veterinary Hospital of UNESP in Botucatu city, Brazil, 25 anemic PCR-positive dogs did not reveal statistical significance (P>0.05) when compared with PCR-negative dogs (3) .…”

Section: Resultsmentioning

confidence: 99%

HEMATOLOGICAL PARAMETERS AND SEROPREVALENCE OF Ehrlichia canis AND Babesia vogeli IN DOGS

et al. 2017

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The aim of this study was to determine the prevalence of anti-Ehrlichia canis and anti-Babesia vogeli IgG antibodies in dogs and correlate this prevalence with risk factors to evaluate the relation of serological status to hematological findings. Blood samples of dogs attended from September 2011 to March 2012 at the veterinary hospital of the Federal University of Lavras, Brazil, were analyzed using an indirect fluorescence antibody test (IFAT). Of the total 160 dog serum samples, 23.7% (38 dogs; CI 95 17.7% -30.7%) were seropositive for E. canis, 40.0% (64 dogs; CI 95 40.0% -59.2%) for B. vogeli, and 5.6% (9 dogs) for both hemoparasites. None of the epidemiological variables showed a significant association (P>0.05) with seropositivity to E. canis and B. vogeli. Dogs seropositive for E. canis showed lower values for hematocrit (P<0.05). However, for the erythrogram, the platelet count, the leukogram and clinical signs, no significant difference (P>0.05) was observed between dogs that were seropositive and seronegative for E. canis or for B. vogeli. Serological results suggest that infection with E. canis and B. vogeli is endemic in the canine population in question, with a prevalence of the subclinical phase (asymptomatic) in dogs that are seropositive for ehrlichiosis or babesiosis. Keywords: babesiosis; canine; ehrlichiosis; IFAT; laboratory findings. ResumoO objetivo deste estudo foi determinar a prevalência de anticorpos IgG anti-Ehrlichia canis e antiBabesia vogeli em cães e correlacionar com a prevalência e fatores de risco para avaliar a relação do estado sorológico com os achados hematológicos. Amostras de sangue de cães coletadas de setembro de 2011 a março de 2012, no Hospital Veterinário da Universidade Federal de Lavras, Brasil, foram analisadas usando o teste de reação de imunofluorescência indireta (RIFI). Do total de amostras de soro de 160 cães, 23,7% (38 cães; IC95 17,7% -30,7%) foram soropositivos para E. canis, 40,0% (64 cães; IC95 40,0% -59,2%) para B. vogeli, e 5,6% (9 cães) para os dois hemoparasitas. Nenhuma das variáveis epidemiológicas mostrou associação significativa (P> 0,05) com a soropositividade para E. canis e B. vogeli. Cães soropositivos para E. canis mostraram valores médios mais baixos para hematócrito (P<0,05). No entanto, para o eritrograma, a contagem de plaquetas, o leucograma e os sinais clínicos, nenhuma diferença significativa (P> 0,05) foi observada entre cães soropositivos e soronegativos para E. canis ou para B. vogeli. Os resultados sorológicos deste estudo sugerem que a Cienc. anim. bras., Goiânia, v.18, 1-9, e-36095, 2017 2 FONSECA, J.P., et al.infecção por E. canis e B. vogeli é endêmica na população canina em questão, com uma prevalência da fase subclínica (assintomática) em cães soropositivos para erliquiose ou babesiose.

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Section: Resultsmentioning

confidence: 99%

HEMATOLOGICAL PARAMETERS AND SEROPREVALENCE OF Ehrlichia canis AND Babesia vogeli IN DOGS

et al. 2017

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“…Immunofluorescent Antibody Test (IFAT) for Ehrlichia canis and Babesia canis -IFAT was performed as described by Camargo (1974) and standardized by Nakaghi et al (2008) for the E. canis system, and as described by Furuta et al (2009), for the B. canis system DNA extraction and nested PCR (nPCR) analysis -Using the commercially available QIAamp ® DNA Blood Mini Kit (cat. No.…”

Section: Methodsmentioning

confidence: 99%

“…For every ten samples, a blank tube containing ultra-pure water (Promega) was included in the DNA extraction, to check the possibility of contamination in this process. A two-step amplification reaction was performed using a thermal cycler (MJ Research; PTC 200), as described by Murphy et al (1998) and Nakaghi et al (2008). DNA extracted from the blood of a dog infected with E. canis (Jaboticabal strain) was used as a positive control.…”

Section: Methodsmentioning

confidence: 99%

“…Canine ehrlichiosis is a disease with high incidence among domestic dogs in Brazil (DAGNONE et al, 2003;NAKAGHI et al, 2008;FARIA et al, 2010;VIEIRA et al, 2011), with extensive mortality. It is a tick-borne disease caused by Gram-negative bacteria, of the species Ehrlichia canis, mostly transmitted by Rhipicephalus sanguineus (LEWIS et al, 1977;TROY;FORRESTER, 1990).…”

Section: Introductionmentioning

confidence: 99%

“…It is a tick-borne disease caused by Gram-negative bacteria, of the species Ehrlichia canis, mostly transmitted by Rhipicephalus sanguineus (LEWIS et al, 1977;TROY;FORRESTER, 1990). This disease has no predilection for age or sex and compromises the host's organic systems in several different ways, with varying degrees of severity (TROY; FORRESTER, 1990;CASTRO et al, 2004;NAKAGHI et al, 2008). Early diagnosis of this infection in dogs can lead to immediate treatment of the disease and, thus, may favor a better outcome.…”

Section: Introductionmentioning

confidence: 99%

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Experimental Ehrlichia canis infection changes acute-phase proteins

Munhoz

Faria

Vargas-Hérnandez

et al. 2012

Rev. Bras. Parasitol. Vet.

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Early diagnosis of canine ehrlichiosis favors prompt institution of treatment and improves the prognosis for the animal, since this disease causes mortality among dogs. Studies have shown that determining the concentration of acute-phase proteins (APPs) may contribute towards early detection of disease and aid in predicting the prognosis. This study aimed to evaluate the APP profile in dogs experimentally infected with Ehrlichia canis, at the start of the infection and after treatment. It also investigated whether any correlation between APP levels and the clinical and laboratory alterations over the course of the disease would be possible. The results obtained showed abnormal levels of all the APPs on the third day after infection (D3), with the highest levels being reached on D18, with the exception of ceruloplasmin and acid glycoprotein, which presented their peaks on D6 and D12 respectively. We concluded that assessment of APP levels could contribute towards establishing an early diagnosis of canine ehrlichiosis, particularly regarding acid glycoprotein and ceruloplasmin, since these proteins were detected at increased levels even before the onset of clinical and laboratory findings of the disease.Keywords: Ehrlichia canis, experimental infection, acute-phase proteins, dogs. ResumoO diagnóstico precoce da erliquiose canina favorece a pronta instituição do tratamento e melhora o prognóstico do animal, pois se trata de uma doença de alta mortalidade em cães. Estudos têm apontado que a determinação da concentração de proteínas de fase aguda (PFA) pode contribuir para detecção precoce de doenças e auxiliar na predição do prognóstico. O presente estudo objetivou avaliar o perfil de proteínas de fase aguda (PFA) em cães experimentalmente infectados com Ehrlichia canis, no início da infecção e após o tratamento. Além disso, se seria possível associá-las com as alterações clínico-laboratoriais durante o curso da doença. Os resultados obtidos evidenciaram que todas as PFA estudadas alteraram suas concentrações em D3 (dia 3), comparadas ao D0, atingindo concentrações máximas em D18, com exceção da ceruloplasmina e da glicoproteína ácida, cujos picos foram observados em D6 e D12, respectivamente. Concluímos que a avaliação das concentrações de PFA poderiam contribuir para o diagnóstico precoce da erliquiose canina, principalmente com relação à ceruloplasmina e glicoproteína ácida, pois seus aumentos foram anteriores ao aparecimento dos sinais clínicos e das alterações laboratoriais da doença.Palavras-chave: Ehrlichia canis, infecção experimental, proteínas de fase aguda, cães.

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Diagnostic value of procalcitonin, C-reactive protein, and leukocyte count in canine ehrlichiosis and canine demodicosis

et al. 2022

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Canine ehrlichiosis: clinical, hematological, serological and molecular aspects

Abstract: The aim of the present study was to compare the direct detection methods of Ehrlichia canis (blood smears and nested PCR), serological tests (Dot-ELISA and

Cited by 97 publications

References 15 publications

HEMATOLOGICAL PARAMETERS AND SEROPREVALENCE OF Ehrlichia canis AND Babesia vogeli IN DOGS

HEMATOLOGICAL PARAMETERS AND SEROPREVALENCE OF Ehrlichia canis AND Babesia vogeli IN DOGS

Experimental Ehrlichia canis infection changes acute-phase proteins

Diagnostic value of procalcitonin, C-reactive protein, and leukocyte count in canine ehrlichiosis and canine demodicosis

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