Canine cystic endometrial hyperplasia and pyometra may downregulate neuropeptide phoenixin and GPR173 receptor expression

Rybska, Marta; Billert, Maria; Skrzypski, Marek; Kubiak, Magdalena; Woźna-Wysocka, Magdalena; Łukomska, Anna; Nowak, Tomasz; Błaszczyk-Cichoszewska, Joanna; Pomorska‐Mól, Małgorzata; Wąsowska, Barbara

doi:10.1016/j.anireprosci.2022.106931

Cited by 6 publications

(8 citation statements)

References 20 publications

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“…However, in women with PCOS, Ullah et al [16] observed a positive correlation between the levels of PNX-14 and LH, FSH, total testosterone, and P 4 , and a negative correlation between the levels of PNX-14 and E 2 and insulin. Similar to our findings, Rybska et al [18] found that the plasma PNX concentration is negatively correlated with P 4 in healthy bitches. On the other hand, in cultures of immortalized hypothalamic neurons, estrogen and leptin do not alter the expression of Smim20 [42].…”

Section: Discussionsupporting

confidence: 93%

Phoenixin-14 as a novel direct regulator of porcine luteal cell functions

Mlyczyńska,

Kurowska,

Wachowska

et al. 2023

Biology of Reproduction

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Phoenixin is a neuropeptide with a well-established role in the central regulation of reproductive processes; however, knowledge regarding its role in the ovary is limited. One of the main active phoenixin isoforms is phoenixin-14, which acts through G protein–coupled receptor 173. Our research hypothesis was that phoenixin-14 is expressed in porcine corpus luteum and exerts luteotropic action by affecting the endocrine function of luteal cells through G protein–coupled receptor 173 and protein kinase signaling. Luteal cells were cultured to investigate the effect of phoenixin-14 (1–1000 nM) on endocrine function. We showed that phoenixin-14 and G protein–coupled receptor 173 are produced locally in porcine corpus luteum and their levels change during the estrous cycle. We detected phoenixin-14 immunostaining in the cytoplasm and G protein–coupled receptor 173 in the cell membrane. Plasma phoenixin levels were highest during the early luteal phase. Interestingly, insulin, luteinizing hormone, progesterone, and prostaglandins decreased phoenixin-14 levels in luteal cells. Phoenixin-14 increased progesterone, estradiol, and prostaglandin E2 secretion, but decreased prostaglandin F2α, upregulated the expression of steroidogenic enzymes, and downregulated receptors for luteinizing hormone and prostaglandin. Also, phoenixin-14 increased the expression of G protein–coupled receptor 173 and the phosphorylation of extracellular signal-regulated kinase 1/2, protein kinase B, inhibited the phosphorylation of protein kinase A, and had mixed effect on AMP-activated protein kinase alpha and protein kinase C. G protein–coupled receptor 173 and extracellular signal-regulated kinase 1/2 mediated the effect of phoenixin-14 on endocrine function of luteal cells. Our results suggest that phoenixin is produced by porcine luteal cells and can be a new regulator of their function.

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Section: Discussionsupporting

confidence: 93%

Phoenixin-14 as a novel direct regulator of porcine luteal cell functions

Mlyczyńska,

Kurowska,

Wachowska

et al. 2023

Biology of Reproduction

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“…C/EBP and OCT-1 are two essential transcription factors of the GnRH promoter (Schalla and Stengel, 2018;Treen A. K. et al, 2016). Rybska et al found that the development of canine uterine disorders, including endometrial hyperplasia or pyometra induced a downregulation of phoenixin and its receptor GPR173 expression (Rybska et al, 2022). Phoenixin can activate the cAMP/PKA pathway and lead to CREB phosphorylation through GPR173, and it induced an increase expression of genes related to follicular development (Xuan Phuoc, et al, 2019).…”

Section: Reproductive Functionmentioning

confidence: 99%

Regulation and physiological functions of phoenixin

Liu

Zhao

et al. 2022

Front. Mol. Biosci.

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Phoenixin is a newly discovered neuropeptide generated from small integral membrane protein 20. Phoenixin is a ligand for the G protein-coupled receptor 173 (GPR173) and has been detected in central and peripheral tissues of human, rats, mice, bovine, and zebrafish. It was initially involved in regulating reproductive function by stimulating the luteinizing hormone release from pituitary cells by increasing the level of gonadotropin-releasing hormone. Recently, many functions of phoenixin have been generalized, including regulation of food intake, memory, Alzheimer’s disease, anxiety, inflammation, neuronal and microglial activity, energy metabolism and body fluid balance, cardiovascular function, and endocrine activity. In addition, the interaction between phoenixin and nesfatin-1 have been revealed. The present article summarized the latest research progress on physiological function of phoenixin, suggesting that it is a potential target for novel drug development and clinical application.

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“…A hiperplasia endometrial cística (HEC) e a piometra são condições anormais comumente reportadas em cadelas e gatas não estéreis (Hagman, 2017;Rybska et al, 2022) e com menor frequência em outras fêmeas de estimação (Hagman, 2022). Podem ocorrer separadamente ou associadas e, neste último caso, a condição é definida como complexo da hiperplasia endometrial cística piometra (HEC-piometra).…”

Section: Introductionunclassified

“…Em casos suspeitos de piometra, o atendimento veterinário deve ser rápido e preciso, uma vez que o estado da paciente pode piorar rapidamente (Hagman et al, 2006;Jitpean et al, 2012;Rybska et al, 2022). Sendo assim, o objetivo deste trabalho é relatar a eficácia do tratamento conservador em uma cadela diagnosticada com piometra e posterior retomada da capacidade reprodutiva.…”

Section: Introductionunclassified

Tratamento conservador para piometra aberta com aglepristone em matriz reprodutiva premiada da raça Buldogue Francês

Dadona

Pereira²,

Oliveira³

et al. 2023

RSD

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A piometra é a doença infecciosa mais comum do sistema reprodutor de cadelas. É considerada uma condição de severa complexidade e quando não diagnosticada e tratada em tempo oportuno pode levar a fêmea ao óbito. Este artigo objetiva relatar a eficácia do tratamento conservador em uma cadela diagnosticada com piometra e sua posterior retomada da capacidade reprodutiva. Trata-se de uma cadela de 12 meses de idade, da raça Buldogue Francês, que apresentava apatia, desidratação, hipertermia, hiporexia, abdominalgia e descarga mucopurulenta vulvar. O diagnóstico presuntivo de piometra baseou-se pelo extravasamento de conteúdo purulento pela vulva, sendo confirmado pela ultrassonografia. O tratamento instituído foi baseado na administração de aglepristone (Alizin® Virbac) e associações com antibioticoterapia, antiflogístico, analgésico e protetor gástrico, mostrando se eficiente para cura clínica do animal. Após três anos, animal foi submetido a inseminação artificial, sendo confirmada a gravidez, manutenção da gestação sem intercorrências e nascimento de dois filhotes. O tratamento em questão foi considerado efetivo pois promoveu a cura clínica do animal, involução completa do útero e retorno da viabilidade reprodutiva.

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Canine cystic endometrial hyperplasia and pyometra may downregulate neuropeptide phoenixin and GPR173 receptor expression

Cited by 6 publications

References 20 publications

Phoenixin-14 as a novel direct regulator of porcine luteal cell functions

Phoenixin-14 as a novel direct regulator of porcine luteal cell functions

Regulation and physiological functions of phoenixin

Tratamento conservador para piometra aberta com aglepristone em matriz reprodutiva premiada da raça Buldogue Francês

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