Canine CD8 T cells showing NK cytotoxic activity express mRNAs for NK cell-associated surface molecules

Lin, Yi Chun; Huang, Yann-Chang; Wang, Yu Shan; Juang, Rong-Huay; Liao, Kuang‐Wen; Chu, Ruiai

doi:10.1016/j.vetimm.2009.07.013

Cited by 27 publications

(31 citation statements)

References 36 publications

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“…Moreover, it has been demonstrated that this subset of canine CD8 ? T-cells derived from peripheral blood lymphocytes referred as lymphokine (IL-2)-activated killers (LAKs) contain substantially higher levels of mRNAs for NK cell-related receptors [52].…”

Section: Discussionmentioning

confidence: 99%

“…The tumoricidal capacity of NK-cells can be increased by cytokines, including interferon, IL-2, and tumor necrosis factor-alpha [54]. Even in the absence of previous sensitization, NK-cells can kill tumor cells that lack or have modified MHC class I surface molecules [52]. The NK-cells exert an action independent of the expression of MHC and then are activated to eliminate the target cells depending on the balance between stimulating and inhibitory signals from its receptors [55].…”

Section: Discussionmentioning

confidence: 99%

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Understanding of the immunological heterogeneity of canine mammary carcinomas to provide immunophenotypic features of circulating leukocytes as clinically relevant prognostic biomarkers

Estrela‐Lima

Araújo²,

Costa-Neto

et al. 2011

Breast Cancer Res Treat

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We have evaluated the phenotypic features of peripheral blood leukocytes as putative novel biomarkers with prognostic values to monitor canine mammary carcinomas. Female dogs were categorized into distinct groups, referred as mammary carcinoma in benign mixed tumor-MC-BMT and mammary carcinoma-MC. Our findings demonstrate that decreased percentage of B-cells along with increased frequency of NK-cells, CD8(+)T-cells, and CD8(+)CD5(Low+)T-cells beside higher T/B-cells and lower CD4(+)/CD8(+) ratio were the hallmarks of MC-BMT. Despite the lower expression of MHCI and MHCII, the lymphocytes from MC-BMT and MC displayed higher migration potential as suggested by enhanced frequency of CD18(+) events. Although increased levels of macrophage-like cells/(CD14(+)CD16(+)) and decreased levels of MHCII expression were a common phenotypic feature in mammary carcinoma, down-regulation of MHCI was selectively observed in MC. Decreased frequency of CD4(+) T-cells with increased levels of CD8(+) T-cells and lower CD4(+)/CD8(+) T-cell ratio were relevant biomarkers of MC-BTM(-). Although decreased expression of MHCI by monocytes was observed in MC-BTM regardless of the presence of lymph node metastasis, this phenotypic feature was restricted to MC free of metastasis. The CD4(+)/CD8(+) T-cells ratio lower than 1.8 was elected as a valid parameter with outstanding performance to predict survival in MC-BMT. On the other hand, the MHCI expression by monocytes higher than 10(2) MFI showed good value to estimate worse outcome in MC. These results should help to improve our understanding of the immunological heterogeneity of canine mammary carcinomas and provide tools for the determination of cut-off scores of clinically relevant immonophenotypic prognostic biomarkers.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Understanding of the immunological heterogeneity of canine mammary carcinomas to provide immunophenotypic features of circulating leukocytes as clinically relevant prognostic biomarkers

Estrela‐Lima

Araújo²,

Costa-Neto

et al. 2011

Breast Cancer Res Treat

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“…Although adaptive immunity is a specific system controlled by T and B cells, innate immunity is a nonspecific system consisting of monocytes, macrophages, polymorphonuclear neutrophilic leukocytes, and (The same applies to the following.) Natural killer (NK) cells [6,11]. The NK cytotoxic activity is a measure of the cytotoxic targeting of tumor cells and is mediated by NK cells, NK T cells, killer T-lymphocytes, CD8 + T cells and possibly other cells [3,6,15].…”

mentioning

confidence: 99%

“…Natural killer (NK) cells [6,11]. The NK cytotoxic activity is a measure of the cytotoxic targeting of tumor cells and is mediated by NK cells, NK T cells, killer T-lymphocytes, CD8 + T cells and possibly other cells [3,6,15]. The NK cytotoxic activities are considered to have important functions in the defense against oncogenicity and cancer metastasis, as they strengthen immunity against tumors [5,15].…”

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confidence: 99%

Influence of General Anesthesia with Isoflurane Following Propofol-Induction on Natural Killer Cell Cytotoxic Activities of Peripheral Blood Lymphocytes in Dogs

et al. 2013

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ABSTRACT. To investigate influence of general anesthesia on immunological anti-tumor activity, the natural killer (NK) cytotoxic activity of peripheral lymphocytes (PBLs) was measured in 7 dogs anesthetized for 3 hr with isoflurane following propofol-induction (anesthesia group) and 6 dogs without anesthesia (control group). Blood samples were collected before (baseline) and 24, 120 and 192 hr after the anesthesia. The PBLs were isolated via centrifugation with Ficoll-Hypaque solution (density, 1.073), and adherent cells were removed. The NK cytotoxic activity of the isolated PBLs against canine thyroid cancer cells was detected by the colorimetric rose Bengal assay. Significant decrease in the NK cytotoxic activity was observed at 24 hr after the anesthesia, compared with the baseline values and the control group. The NK cytotoxic activities were recovered to the baseline values until 120 hr after the anesthesia. The general anesthesia with isoflurane following propofol-induction decreased the NK cytotoxic activities of PBLs in dogs. This finding has a clinical relevance to the risk of tumor recurrence or metastasis induced by the suppression of immunological anti-tumor activity after general anesthesia in dogs. The results further emphasized the importance of the need to evaluate immune suppression following general anesthesia in animals.

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“…There are only a few recently published reports which identify a potential role for the CD8(+) subpopulation in NK cell activity in dogs. Lin et al (2010) used CD8(+) cells derived from PBLs and lymphokine (IL-2)-activated killers (LAKs) of PBLs, which were CD3(+), CD4(-) CD21(-), CD5(lo), alpha/beta TCR (+) and gamma/delta TCR(-). They found an increased mRNA expression for NK cell-specific receptors, such as NKp30, NKp44, NKG2D and CD16, for PBLs and NKG2D and CD56 for LAKs, as compared to CD8(-) cells.…”

Section: The Identification Of Canine CD Leukocyte Antigen Family Marmentioning

confidence: 99%

The immunological, biochemical and molecular bases of canine senescence and carcinogenesis: a review

Woźna¹,

Kempisty²,

Piotrowska³

et al. 2012

Vet. Med. - Czech

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ABSTRACT:Senescence is a complex set of processes involving several biochemical, molecular and metabolomic changes, including also many disturbances in the immunological system. There are many factors, described as intrinsic and extrinsic (environmental), that may lead to advanced body senescence. In this review, several of the biochemical as well as molecular factors involved in senescence are described. The importance of immunological deficiencies as well as changes in the immunological response after induction of senescence is also highlighted. Furthermore, the molecular basis of canine carcinogenesis in relation to interleukin expression and activation as well as the role of CD leukocyte common antigen in the identification of cancer development and progression, are also described. Keywords: senescence; carcinogenesis; canine List of abbreviations4-HNE = 4-hydroxynonenal, Aβ1-42 = β-amyloid form, AD = Alzheimer disease, alpha(1)AG = alpha 1 acid glycoprotein, bcl-2 = B-cell leukemia 2, BER = base-excision repair, BRCA1 = breast cancer susceptibility gene-1, CD = cluster of differentiation, coating the surface of B lymphocytes and T lymphocytes, CTVT = canine transmissible venereal tumour, FOXP3 = forkhead box P3, scurfin, IFN-gamma = interferon-gamma, IL = interleukin, IVL = intravascular lymphoma, MGT's = mammary gland tumours, MHC = major histocompatibility complex, NFTs = neurofibrillary tangles, NHL = non-Hodgkin's lymphoma, NK = natural killer, OGG1 = 8-oxoguanine glycosylase 1, OSA = osteosarcoma, PAC-1 = first procaspase activating compound, PBLs = peripheral blood lymphocytes, pIL = interleukin plasmids, SLC1A3 = solute carrier family 1 (glial high affinity glutamate transporter), member 3, SOD = superoxide dismutase, TCR = T-cell receptor, TCRBCL = T-cell rich B-cell lymphoma, TGF-beta = transforming growth factor beta, TIL's = tumour-infiltrating lymphocytes, Tregs = T regulatory cells, VEGF = vascular endothelial growth factor

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Canine CD8 T cells showing NK cytotoxic activity express mRNAs for NK cell-associated surface molecules

Cited by 27 publications

References 36 publications

Understanding of the immunological heterogeneity of canine mammary carcinomas to provide immunophenotypic features of circulating leukocytes as clinically relevant prognostic biomarkers

Understanding of the immunological heterogeneity of canine mammary carcinomas to provide immunophenotypic features of circulating leukocytes as clinically relevant prognostic biomarkers

Influence of General Anesthesia with Isoflurane Following Propofol-Induction on Natural Killer Cell Cytotoxic Activities of Peripheral Blood Lymphocytes in Dogs

The immunological, biochemical and molecular bases of canine senescence and carcinogenesis: a review

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