Canine and Feline Spontaneous Mammary Tumours as Models of Human Breast Cancer

Vilhena, Hugo; Figueira, Ana Catarina; Schmitt, Fernando; Canadas, Ana; Chaves, Raquel; Gama, Adelina; Dias‐Pereira, Patrícia

doi:10.1007/978-3-030-30734-9_9

Cited by 8 publications

(9 citation statements)

References 248 publications

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“…Breast carcinoma was the second most incident cancer (2.2 million new cases), causing 685,000 human deaths in 2020 [ 2 ]. Something similar can be observed among companion animals, especially dogs, since most tumors in uncastrated female dogs are mammary [ 3 , 4 ], with 50 to 75% being malignant [ 4 ].…”

Section: Introductionmentioning

confidence: 86%

Transcriptomic Profile of Canine Mammary Ductal Carcinoma

Santos

Fernández

Pardini

et al. 2023

IJMS

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Dogs can be excellent models for spontaneous studies about breast cancers, presenting similarities in clinical behavior and molecular pathways of the disease. Thus, analyses of the canine transcriptome can identify deregulated genes and pathways, contributing to the identification of biomarkers and new therapeutic targets, benefiting humans and animals. In this context, this study aimed to determine the transcriptional profile of canine mammary ductal carcinoma and contribute to the clarification of the importance of deregulated molecules in the molecular pathways involved in the disease. Therefore, we used mammary ductal carcinoma tissue samples and non-tumor mammary tissue from the radical mastectomy of six female dogs. Sequencing was performed on the NextSeq-500 System platform. A comparison of carcinoma tissue and normal tissue revealed 633 downregulated and 573 upregulated genes, which were able to differentiate the groups by principal component analysis. Gene ontology analysis indicated that inflammatory, cell differentiation and adhesion, and extracellular matrix maintenance pathways were mainly deregulated in this series. The main differentially expressed genes observed in this research can indicate greater disease aggressiveness and worse prognosis. Finally, the study of the canine transcriptome indicates that it is an excellent model to generate information relevant to oncology in both species.

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Section: Introductionmentioning

confidence: 86%

Transcriptomic Profile of Canine Mammary Ductal Carcinoma

Santos

Fernández

Pardini

et al. 2023

IJMS

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“…Cancer rates have increased in humans and domestic animals, making this disease one of the leading causes of death worldwide in these species [ 72 ]. The access to vaccines, better nutrition and veterinary care, as well as a greater interest of pet owners are allowing dogs to live longer, which contribute to the increase in cancer cases in company animals [ 73 ].…”

Section: Breast/mammary Cancermentioning

confidence: 99%

“…Thus, depending on the objectives of each experiment, researchers should select the model that best suits the scientific question to be investigated. Clinically, CMTs share many similarities with breast cancer in women in terms of histopathology, biological behavior, hormone dependence, risk factors and genetic alterations [ 3 , 72 , 198 , 199 , 200 ]. Dogs often share the same environment as their owners, and in some cases exhibit similar co-morbidities [ 72 ].…”

Section: Advantages and Disadvantages Of Spontaneous Tumors And Induc...mentioning

confidence: 99%

Mammary Glands of Women, Female Dogs and Female Rats: Similarities and Differences to Be Considered in Breast Cancer Research

Ferreira

Gama

Seixas

et al. 2023

Veterinary Sciences

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Breast cancer is one of the most common and well-known types of cancer among women worldwide and is the most frequent neoplasm in intact female dogs. Female dogs are considered attractive models or studying spontaneous breast cancer, whereas female rats are currently the most widely used animal models for breast cancer research in the laboratory context. Both female dogs and female rats have contributed to the advancement of scientific knowledge in this field, and, in a “One Health” approach, they have allowed broad understanding of specific biopathological pathways, influence of environmental factors and screening/discovery of candidate therapies. This review aims to clearly showcase the similarities and differences among woman, female dog and female rat concerning to anatomical, physiological and histological features of the mammary gland and breast/mammary cancer epidemiology, in order to better portray breast tumorigenesis, and to ensure appropriate conclusions and extrapolation of results among species. We also discuss the major aspects that stand out in these species. The mammary glands of female dogs and women share structural similarities, especially with respect to the lactiferous ducts and lymphatic drainage. In contrast, female rats have only one lactiferous duct per nipple. A comprehensive comparison between humans and dogs is given a special focus, as these species share several aspects in terms of breast/mammary cancer epidemiology, such as age of onset, hormonal etiology, risk factors, and the clinical course of the disease. Holistically, it is clear that each species has advantages and limitations that researchers must consider during the development of experimental designs and data analysis.

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“…For these reasons, the CMT is considered to be a valuable and reliable human breast cancer model [ 3 ]. In addition, other companion animals, for example, cats, are increasingly used as breast cancer models [ 4 ]. Many similarities have been demonstrated in the clinical behavior of the disease, etiology, and molecular subtypes of tumors between feline and human breast cancer [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

“…Many similarities have been demonstrated in the clinical behavior of the disease, etiology, and molecular subtypes of tumors between feline and human breast cancer [ 5 ]. Moreover, strong changes in the immune response are observed in dogs and cats, similar to those in humans [ 4 , 6 , 7 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

IFN-λ Modulates the Migratory Capacity of Canine Mammary Tumor Cells via Regulation of the Expression of Matrix Metalloproteinases and Their Inhibitors

Pingwara

Kosmala

Woźniak

et al. 2021

Cells

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Interactions between neoplastic and immune cells taking place in tumors drive cancer regulatory mechanisms both in humans and animals. IFN-λ, a potent antiviral factor, is also secreted in the tumor; however, its role in tumor development is still unclear. In our study, we investigate the influence of IFN-λ on the canine mammary tumor (CMT) cell survival and their metastatic potential in vitro. First, we examined, by Western blot, the expression of the IFN-λ receptor complex in three CMT cell lines (P114, CMT-U27 and CMT-U309). We showed that only two cell lines (P114 and CMT-U27) express both (IL-28RA and IL-10Rb) receptor subunits and respond to IFN-λ treatment by STAT phosphorylation and the expression of interferon-stimulated genes. Using MTT, crystal violet and annexin-V assays, we showed a minimal role of IFN-λ in CMT viability. However, IFN-λ administration had a contradictory effect on cell migration in the scratch test, namely, it increased P114 and decreased CMT-U27 motility. Moreover, we demonstrated that this process is related to the expression of extracellular matrix metalloproteinases and their inhibitors; furthermore, it is independent of Akt and ERK signaling pathways. To conclude, we showed that IFN-λ activity is reliant on the expression of two receptor subunits and tumor type, but further investigations are needed.

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Canine and Feline Spontaneous Mammary Tumours as Models of Human Breast Cancer

Cited by 8 publications

References 248 publications

Transcriptomic Profile of Canine Mammary Ductal Carcinoma

Transcriptomic Profile of Canine Mammary Ductal Carcinoma

Mammary Glands of Women, Female Dogs and Female Rats: Similarities and Differences to Be Considered in Breast Cancer Research

IFN-λ Modulates the Migratory Capacity of Canine Mammary Tumor Cells via Regulation of the Expression of Matrix Metalloproteinases and Their Inhibitors

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