‘Candidatus Mycoplasma haematohydrochoerus’, a novel hemoplasma species in capybaras (Hydrochoerus hydrochaeris) from Brazil

Vieira, Rafael Felipe da Costa; Santos, Nelson Jessé Rodrigues dos; Valente, Jessica Damiana Marinho; Santos, Leonardo Pereira dos; Lange, Rogério Ribas; Duque, Juan Carlos; Ferrari, Marcos Vinícius; Filho, Ivan Roque de Barros; Collere, Flávia Carolina Meira; Ferrari, Larissa Dantas Roeder; Gonçalves, Luiz Ricardo; Sanches, Gustavo Seron; André, Marcos Rogério; Vieira, Thállitha Samih Wischral Jayme

doi:10.1016/j.meegid.2021.104988

Cited by 17 publications

(14 citation statements)

References 28 publications

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“…Based mainly on the partial 16S rRNA and 23S rRNA genes amplification, novel species of hemotropic Mycoplasma spp. infecting capybaras [ 22 ], hairy dwarf porcupines [ 23 ], opossums [ 24 ], and coatis [ 25 ] have been proposed. When analyzing the topologies found by partial 16S rRNA gene phylogeny from these reports, sequences from each novel Candidatus species were positioned separately in clades inside the “ Mycoplasma haemofelis group”, with satisfactory bootstrap values for species separation.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, newly described genotypes and Candidatus species have been reported in wild animals from Brazil based on PCR amplification of selected molecular markers, namely 16S rRNA and 23S rRNA genes. Potentially novel species have been identified in capybaras ( Hydrochoerus hydrochaeris ) [ 22 ] hairy dwarf porcupines ( Sphiggurus villosus ) [ 23 ], opossums ( Didelphis albiventris ) [ 24 ], and coatis ( Nasua nasua ) [ 25 ] from Brazil. Moreover, putative new genotypes have also been reported in free-ranging animals from different regions of the country, such as non-human primates from the Brazilian Amazon [ 26 ] rodents from several Brazilian biomes [ 27 ] and bats [ 28 , 29 ].…”

Section: Introductionmentioning

confidence: 99%

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Expanding the Universe of Hemoplasmas: Multi-Locus Sequencing Reveals Putative Novel Hemoplasmas in Lowland Tapirs (Tapirus terrestris), the Largest Land Mammals in Brazil

et al. 2022

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The lowland tapir (Tapirus terrestris) is the largest land mammal in Brazil and classified as a vulnerable species, according to the assessment of the risk of extinction. The present study aimed at investigating the occurrence and genetic diversity of hemoplasmas in free-ranging T. terrestris from the Brazilian Pantanal and Cerrado biomes. Blood samples were collected from 94 living and eight road-killed tapirs, totalizing 125 samples Conventional PCR targeting four different genes (16S rRNA, 23S rRNA, RNAse P, and dnaK) were performed, and the obtained sequences were submitted for phylogenetic, genotype diversity, and distance analyses. The association between hemoplasma positivity and possible risk variables (age, gender, and origin) was assessed. Out of 122 analyzed samples, 41 (41/122; 33.61% CI: 25.84–42.38%) were positive in the 16S rRNA-based PCR assay for hemoplasmas. Positivity for hemoplasmas did not differ between tapirs’ gender and age. Tapirs from Pantanal were 5.64 times more likely to present positive results for hemoplasmas when compared to tapirs sampled in Cerrado. BLASTn, phylogenetic, genotype diversity, and distance analyses performed herein showed that the sampled lowland tapirs might be infected by two genetically distinct hemoplasmas, namely ‘Candidatus Mycoplasma haematoterrestris’ and ‘Candidatus Mycoplasma haematotapirus’. While the former was positioned into “Mycoplasma haemofelis group” and closely related to ‘Candidatus Mycoplasma haematoparvum, the latter was positioned into “Mycoplasma suis group” and closely related to ‘Candidatus Mycoplasma haematobos’. The impact of both putative novel species on tapir health status should be investigated.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Expanding the Universe of Hemoplasmas: Multi-Locus Sequencing Reveals Putative Novel Hemoplasmas in Lowland Tapirs (Tapirus terrestris), the Largest Land Mammals in Brazil

et al. 2022

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“…The gapdh gene was consistently amplified in all ring-tailed coati (Barker et al, 2011;Pontarolo et al, 2021;Valente et al, 2020;Vieira et al, 2021). On this pattern, the 23S rRNA sequences amplified in the present study showed only 94.3%-94.1% identity to 'Ca.…”

Section: Resultsmentioning

confidence: 49%

“…A. dubitatum adult ticks' salivary glands (Vieira et al, 2021) found parasitizing hemoplasma-infected capybaras from central-western and southern Brazil, respectively. Unfortunately, ticks infesting ring-tailed coatis were not collected in the present study.…”

Section: Resultsmentioning

confidence: 99%

‘ Candidatus Mycoplasma haematonasua’ and tick‐borne pathogens in ring‐tailed coatis ( Nasua nasua Linnaeus, 1976) from the Iguaçu National Park, Paraná State, southern Brazil

Collere

Delai

Ferrari

et al. 2021

Transbound Emerg Dis

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The Iguaçu National Park (INP) is the largest remnant of Atlantic Forest in southern Brazil, representing an ecological continuum with Argentina. The INP harbours a diverse fauna, with ring-tailed coatis (Nasua nasua Linnaeus, 1976, Carnivora: Procyonidae) in close contact with tourists either begging and/or snatching food from visitors. A potentially novel haemotropic Mycoplasma sp. has been previously detected in the ring-tailed coatis from central-western and southern Brazil. Therefore, the aims of this study were to investigate the occurrence of haemotropic Mycoplasma sp. and tickborne pathogens in wild ring-tailed coatis from the INP, Foz do Iguaçu municipality, Paraná State, southern Brazil. Blood samples were collected from 18 wild ring-tailed coatis and evaluated by conventional PCR (cPCR) assays for haemotropic Mycoplasma spp. (16S and 23S rRNA), Theileria/Babesia spp. (18S rRNA) and Ehrlichia/Anaplasma spp. (16S rRNA, sodB, dsb and groEL). Eight out of 18 (44.44%; 95% CI: 24.56%-66.28%) animals were positive for haemotropic Mycoplasma spp. All ring-tailed coatis tested negative for Theileria/Babesia spp. and only one out of 18 (5.56%; 95% CI: 0.99%-25.76%) animals tested positive for Ehrlichia/Anaplasma spp. by the 16S rRNA cPCR. Unfortunately, multiple attempts to sequence the 16S rRNA gene of the Ehrlichia/Anaplasma-positive sample have failed. Phylogenetic and network analysis of the hemoplasma 16S and 23S rRNA gene fragments confirmed that animals were infected by a potentially novel haemotropic Mycoplasma sp. previously reported in ringtailed coatis from Brazil. The name 'Candidatus Mycoplasma haematonasua' is proposed for this novel organism.

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“…We retrieved blood samples from 17 capybaras and salivary glands from 11 Amblyomma dubitatum ticks from these capybaras that were collected for a previous study conducted in southern Brazil ( 2 ). We screened blood samples by using PCR targeting of the 16S rRNA gene of Ehrlichia and Anaplasma ( 3 , 4 ).…”

mentioning

confidence: 99%

Novel Anaplasmataceae agents Candidatus Ehrlichia hydrochoerus and Anaplasma spp. Infecting Capybaras, Brazil

Vieira¹,

Collere²,

Ferrari³

et al. 2022

Emerg. Infect. Dis.

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We amplified Ehrlichia and Anaplasma DNA from Amblyomma dubitatum tick–infested capybaras ( Hydrochoerus hydrochaeris ) in southern Brazil. Sequencing of 16S rRNA, sodB , and groEL indicated a novel Ehrlichia species, and sequencing of 16S rRNA from 2 capybaras indicated a novel Anaplasma species. The tick vectors remain unknown.

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‘Candidatus Mycoplasma haematohydrochoerus’, a novel hemoplasma species in capybaras (Hydrochoerus hydrochaeris) from Brazil

Cited by 17 publications

References 28 publications

Expanding the Universe of Hemoplasmas: Multi-Locus Sequencing Reveals Putative Novel Hemoplasmas in Lowland Tapirs (Tapirus terrestris), the Largest Land Mammals in Brazil

Expanding the Universe of Hemoplasmas: Multi-Locus Sequencing Reveals Putative Novel Hemoplasmas in Lowland Tapirs (Tapirus terrestris), the Largest Land Mammals in Brazil

‘ Candidatus Mycoplasma haematonasua’ and tick‐borne pathogens in ring‐tailed coatis ( Nasua nasua Linnaeus, 1976) from the Iguaçu National Park, Paraná State, southern Brazil

Novel Anaplasmataceae agents Candidatus Ehrlichia hydrochoerus and Anaplasma spp. Infecting Capybaras, Brazil

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