Candida parapsilosis ( sensu lato ) isolated from hospitals located in the Southeast of Brazil: Species distribution, antifungal susceptibility and virulence attributes

Ziccardi, Mariangela; Souza, Lucieri O.P.; Gandra, Rafael M.; Galdino, Anna Clara Milesi; Baptista, Andréa R.S.; Nunes, Ana Paula Ferreira; Ribeiro, Maria Aparecida; Branquinha, Marta H.; Santos, André Luis Souza dos

doi:10.1016/j.ijmm.2015.08.003

Cited by 55 publications

(58 citation statements)

References 73 publications

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“…Among the azole derivatives analyzed in this study, FLC and VRC showed high levels of efficacy, as a single strain derived from the tongue showed SDD and SI to these drugs, respectively. And the MIC 50 and MIC 90 of both drugs was similar to that reported by other authors [2,17,18].…”

Section: Discussionsupporting

confidence: 89%

Molecular Characterization of Candida parapsilosis Species Complex in Niches of the Oral Cavity in a Cohort of Patients from Argentina with Different Oral and Dental Clinical Manifestations

Rodríguez¹

2016

J. Dent. Sci. Ther

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Candida parapsilosis is considered to be a complex of three species due to genetic heterogeneity. Currently, it is the second most isolated yeast in bloodstream infections and oral cavity sites with C. parapsilosis sensu stricto and C. metapsilosis being the species mostly recovered from oral niches in both healthy and immunecompromised patients respectively. Disparity in antifungal susceptibility has been reported among species of this complex, while no data on distribution and behavior in the oral cavity has been reported in Argentina.Objective: To molecularly characterize a collection of isolates from Argentina, phenotypically identified as C. parapsilosis from different oral niches, in a cohort of patients with different oral and dental clinical conditions. Methodology:This experimental study used 31 yeasts phenotypically recognized as C. parapsilosis and recovered for molecular characterization by end-point PCR using specific primers. The in vitro sensitivity of isolates against azoles, echinocandins, amphotericin and flucytosine was evaluated by automated Vitek2 system and MIC (Minimum Inhibitory Concentration) in line with CLSI (Clinical and Laboratory Standards Institute) M27-A3 guidelines.Results: All isolates, 100% (31/31), were positive for C. parapsilosis sensu stricto species. Of these, 77.4% were recovered in oral inflammatory conditions, and 94.7% of the strains analyzed for antifungal susceptibility profile showed favorable response to all drugs tested. Conclusions:C. parapsilosis sensu strictois a common colonizing of the oral mucosa under pathological conditions, and generally shows favorable response to antifungal agents commonly used in clinical practice, but with a tendency to lower responsiveness to azoles and echinocandins.

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Section: Discussionsupporting

confidence: 89%

Molecular Characterization of Candida parapsilosis Species Complex in Niches of the Oral Cavity in a Cohort of Patients from Argentina with Different Oral and Dental Clinical Manifestations

Rodríguez¹

2016

J. Dent. Sci. Ther

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“…All biofilm assays were conducted 10 times for each isolate 10 . The isolates were classified into four categories: non-producers, weak, moderate, or strong biofilm producers 4 C. orthopsilosis are not usually found or occur at very low frequencies in nosocomial environments; previous reports have suggested they are associated mainly with clinical samples and that their relative frequency varies among hospitals and geographic regions 11,12 .…”

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confidence: 99%

In vitro evaluation of hydrolytic enzyme activity and biofilm formation of Candida parapsilosis species complex from a nosocomial environment

Paula-Mattiello

Oliveira

Medina-Silva

2017

Rev. Soc. Bras. Med. Trop.

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Introduction: Candida parapsilosis complex species, frequently found in hospital environments, have gained importance as etiological agents of candidemia. Methods: Candida parapsilosis complex isolates from a nosocomial environment were identified and their hydrolitic enzyme activity and ability to form biofilm were characterized. Results: Twenty-two C. parapsilosis sensu stricto isolates produced proteinase and three produced phospholipase. Most Candida metapsilosis isolates produced proteinase and one also produced phospholipase. All 29 isolates formed biofilms. Conclusions: The nosocomial environment may act as a reservoir for C. parapsilosis complex isolates with phenotypic features that could possibly lead to nosocomial infections and health complications in hospital patients.

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“…In this complex, C. parapsilosis sensu stricto is the species most frequently recovered from clinical isolates of immunocompetent and immunocompromised patients, both in normal and pathological conditions [7,[41][42][43][44][45][46][47]. This knowledge has been supported by many studies around the world.…”

Section: Discussionmentioning

confidence: 99%

The Oral Cavity: A Reservoir that Favors Colonization and Selection of Candida parapsilosis sensu stricto Strains with High Pathogen Potential Under Conditions of Gingival-periodontal Disease

Rodríguez¹

2018

J. Dent. Sci. Ther

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The Candida parapsilosis complex comprises three genetically related species: Candida parapsilosis sensu stricto, Candida orthopsilosis and Candida metapsilosis. During the last decade, Candida parapsilosis sensu stricto has become the second most commonly isolated yeast from patients with blood stream invasive fungal infections, following Candida albicans. Despite its clinical significance, little is known about its distribution and behavior in oral cavity niches. Given this background, we intended to perform a molecular characterization of 38 isolates of the Candida parapsilosis complex collected from oral cavities of immunocompetent patients with and without gingivalperiodontal disease, with the aim of determining its distribution and establishing a correlation with clinical data. Besides, we assessed the virulence of the strains by examining the in vitro biofilm-forming capacity in two different culture media: YPD and RPMI 1640. Being more frequently isolated from oral cavities with pathological conditions, 89.5% of the strains were reconfirmed to be Candida parapsilosis sensu stricto. More than 70% of the Candida parapsilosis sensu stricto strains showed biofilm-forming capacity in both culture media, reflecting a statistically significant association between biofilm-forming capacity and the clinical conditions of the subjects from whom the strains were isolated. Among the three complex species, Candida parapsilosis sensu stricto is a normal commensal in the oral cavity, being more frequently recovered in inflammatory conditions. Gingival-periodontal disease may be a factor triggering overgrowth and virulence of Candida parapsilosis sensu stricto commensal strains.

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Candida parapsilosis ( sensu lato ) isolated from hospitals located in the Southeast of Brazil: Species distribution, antifungal susceptibility and virulence attributes

Cited by 55 publications

References 73 publications

Molecular Characterization of Candida parapsilosis Species Complex in Niches of the Oral Cavity in a Cohort of Patients from Argentina with Different Oral and Dental Clinical Manifestations

Molecular Characterization of Candida parapsilosis Species Complex in Niches of the Oral Cavity in a Cohort of Patients from Argentina with Different Oral and Dental Clinical Manifestations

In vitro evaluation of hydrolytic enzyme activity and biofilm formation of Candida parapsilosis species complex from a nosocomial environment

The Oral Cavity: A Reservoir that Favors Colonization and Selection of Candida parapsilosis sensu stricto Strains with High Pathogen Potential Under Conditions of Gingival-periodontal Disease

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