Candida glabrata peroxiredoxins, Tsa1 and Tsa2, and sulfiredoxin, Srx1, protect against oxidative damage and are necessary for virulence

Gutiérrez-Escobedo, Guadalupe; Hernández-Carreón, Oscar; Morales-Rojano, Brenda; Revuelta-Rodríguez, Brenda; Vázquez-Franco, Norma; Castaño, Irene; Peñas, Alejandro De Las

doi:10.1016/j.fgb.2019.103287

Cited by 20 publications

(15 citation statements)

References 64 publications

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“…C. glabrata, on the other hand, resists oxidative stress by producing Srx1 (sulfiredoxin) and two peroxiredoxins (Tsa1 and Tsa2). TSA1 and 2 are expressed in the presence of neutrophils [ 80 ].…”

Section: The Virulence Of Candida Sppmentioning

confidence: 99%

Candida and Candidiasis—Opportunism Versus Pathogenicity: A Review of the Virulence Traits

et al. 2020

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One of the most important questions in microbiology nowadays, is how apparently harmless, commensal yeasts like Candida spp. can cause a rising number of infections. The occurrence of the disease requires firstly the attachment to the host cells, followed by the invasion of the tissue. The adaptability translates into a rapid ability to respond to stress factors, to take up nutrients or to multiply under different conditions. By forming complex intracellular networks such as biofilms, Candida spp. become not only more refractive to antifungal therapies but also more prone to cause disease. The inter-microbial interactions can enhance the virulence of a strain. In vivo, the fungal cells face a multitude of challenges and, as a result, they develop complex strategies serving one ultimate goal: survival. This review presents the virulence factors of the most important Candida spp., contributing to a better understanding of the onset of candidiasis and raising awareness of the highly complex interspecies interactions that can change the outcome of the disease.

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Section: The Virulence Of Candida Sppmentioning

confidence: 99%

Candida and Candidiasis—Opportunism Versus Pathogenicity: A Review of the Virulence Traits

et al. 2020

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“…Although the relationship between acetic acid stress tolerance and some target genes remains unclear, genes RGS2 , SIP18 , SRX1 , and YDJ1 were proved to contribute to oxidative stress tolerance [ 46 , 47 , 48 , 49 ]. To explore the roles of Rim15 target genes in relieving oxidative stress, the growth performance of the deletion strains lacking Rim15 target genes was evaluated with the treatment of 5 mM H 2 O 2 .…”

Section: Resultsmentioning

confidence: 99%

Novel Roles of the Greatwall Kinase Rim15 in Yeast Oxidative Stress Tolerance through Mediating Antioxidant Systems and Transcriptional Regulation

Wang,

Yuan,

Zhang

et al. 2024

Antioxidants

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The Greatwall-family protein kinase Rim15 is associated with the nutrient starvation response, whereas its role in oxidative stress responses remains unclear. Here, acetic acid and peroxide were used as two oxidative stress elicitors. The antioxidant indicator assay under acetic acid stress revealed the impaired growth in rim15Δ related to the regulation of antioxidant systems. Comparative transcriptome analysis revealed that differentially expressed genes (DEGs) are predicted to be mostly regulated by oxidative stress-responsive transcriptional factor Yap1. Among the DEGs, acetic acid stress-induced genes were found, and YAP1 disruption also inhibited their induction. The deletion of Rim15 or the Rim15 kinase domain in yap1Δ did not further decrease the gene expression, suggesting that Rim15 functions together with Yap1 in regulating acetic acid stress-induced genes, which requires Rim15 kinase activity. Additionally, Rim15 regulated H2O2 stress tolerance through partially similar but special mechanisms in that Rim15 kinase activity impacted acetic acid and H2O2 stress tolerance in different degrees, indicating the different mechanisms underlying Rim15-mediated redox regulation against different stressors. These results benefit the better understanding of stress signaling pathways related to Rim15. Given that Rim15 and some of its target genes are conserved across eukaryotes, these results also provide a basis for studies of oxidative stress-related processes in other organisms.

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“…Generally, different Prxs might have different functions in the cell and not necessarily all Prxs are induced equally by oxidative stress. In Candida glabrata and S. cerevisiae , one Prx was described as the main Prx against ROS, while another was proposed to be a backup [ 16 , 31 ]. Alternatively, Prx-2 and Prx-3 might fulfil other functions, since Prxs were also reported to contribute to genome stability or function as chaperones reviewed by de Oliveira et al [ 8 ].…”

Section: Discussionmentioning

confidence: 99%

Transcriptional changes of proteins of the thioredoxin and glutathione systems in Acanthamoeba spp. under oxidative stress – an RNA approach

et al. 2022

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The thioredoxin (Trx) and the glutathione (GSH) systems represent important antioxidant systems in cells and in particular thioredoxin reductase (TrxR) has been shown to constitute a promising drug target in parasites. For the facultative protozoal pathogen Acanthamoeba, it was demonstrated that a bacterial TrxR as well as a TrxR, characteristic of higher eukaryotes, mammals and humans is expressed on the protein level. However, only bacterial TrxR is strongly induced by oxidative stress in Acanthamoeba castellanii. In this study, the impact of oxidative stress on key enzymes involved in the thioredoxin and the glutathione system of A. castellanii under different culture conditions and of clinical Acanthamoeba isolates was evaluated on the RNA level employing RT-qPCR. Additionally, the effect of auranofin, a thioredoxin reductase inhibitor, already established as a potential drug in other parasites, on target enzymes in A. castellanii was investigated. Oxidative stress induced by hydrogen peroxide led to significant stimulation of bacterial TrxR and thioredoxin, while diamide had a strong impact on all investigated enzymes. Different strains displayed distinct transcriptional responses, rather correlating to sensitivity against the respective stressor than to respective pathogenic potential. Culture conditions appear to have a major effect on transcriptional changes in A. castellanii. Treatment with auranofin led to transcriptional activation of the GSH system, indicating its role as a potential backup for the Trx system. Altogether, our data provide more profound insights into the complex redox system of Acanthamoeba, preparing the ground for further investigations on this topic.

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Candida glabrata peroxiredoxins, Tsa1 and Tsa2, and sulfiredoxin, Srx1, protect against oxidative damage and are necessary for virulence

Cited by 20 publications

References 64 publications

Candida and Candidiasis—Opportunism Versus Pathogenicity: A Review of the Virulence Traits

Candida and Candidiasis—Opportunism Versus Pathogenicity: A Review of the Virulence Traits

Novel Roles of the Greatwall Kinase Rim15 in Yeast Oxidative Stress Tolerance through Mediating Antioxidant Systems and Transcriptional Regulation

Transcriptional changes of proteins of the thioredoxin and glutathione systems in Acanthamoeba spp. under oxidative stress – an RNA approach

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