Candida dubliniensis: Ten years on

Sullivan, Derek J.; Moran, Gary P.; Coleman, David C.

doi:10.1016/j.femsle.2005.09.015

Cited by 112 publications

(105 citation statements)

References 67 publications

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“…The proposed identification approach provides an alternative tool for the differentiation between the closely related C. albicans and C. dubliniensis. C. dubliniensis was described as being more prevalent among immunocompromised and HIV infected populations [32].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Pyrosequencing® technology for the identification of clinically relevant non-dematiaceous yeasts and related species

Montero

Shea

Jones

et al. 2008

Eur J Clin Microbiol Infect Dis

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Pyrosequencing was used to identify 133 isolates of clinically relevant non-dematiaceous yeasts. These included 97 ATCC strains (42 type strains), 7 UAMH strains, and 29 clinical isolates. Isolates belonged to the following genera: Candida (18 species), Trichosporon (10), Cryptococcus (7), Malassezia (3), Rhodotorula (2), Geotrichum (1), Blastoschizomyces (1) and Kodamaea (1). Amplicons were obtained of a hypervariable ITS region and analyzed using Pyrosequencing technology. The data were evaluated by a BLAST search against the GenBank database and correlated with data obtained by conventional cycle sequencing of the ITS1-5.8S-ITS2 region. Cycle sequencing identified (78.9%) if the isolates to the species level. Pyrosequencing technology identified 69.1%. In 90.1% of all the strains tested, identification results of both sequencing methods were identical. Most Candida isolates can be identified to the species level by pyrosequencing. Trichosporon species and some Cryptococcus cannot be differentiated at the species level. Pyrosequencing can be used for the reliable identification of most commonly isolated nondematiaceous yeasts, with a reduction of cost per identification compared to conventional sequencing.

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Section: Discussionmentioning

confidence: 99%

Evaluation of Pyrosequencing® technology for the identification of clinically relevant non-dematiaceous yeasts and related species

Montero

Shea

Jones

et al. 2008

Eur J Clin Microbiol Infect Dis

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“…dubliniensis is opportunistic yeast, and it appears to have a worldwide distribution [20,21]. Despite the effort that has been expended to identify C. dubliniensis in clinical samples, a definitive assessment of the prevalence of this species is still lacking [2]. C. dubliniensis have been isolated from a wide range of geographical locations including Europe, North and South America, and Australia [22].…”

Section: Discussionmentioning

confidence: 99%

“…Although Candida is consisting of more than 200 species, only a few of them has been associated with human infection [2]. Of these, C. albicans is the most important infectious agent and represents a serious public health challenge.…”

Section: Introductionmentioning

confidence: 99%

Incidence and Virulence Traits of Candida Dubliniensis Isolated From Clinically Suspected Patients

Vinodhini¹,

Moorthy²,

Mickymaray³

2016

Asian J Pharm Clin Res

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Objective: Fungal infections are caused by Candida species are increasing mainly in immunocompromised patients. Among Candida species, Candida dubliniensis has recently increasing opportunistic pathogenic yeast. The present study was aimed to assess the incidence and virulence factors of C. dubliniensis isolated from urine samples of various hospitalized clinically suspected patients.Methods: Totally 1,406 urine samples were processed, among that 9 (0.64%) were identified as C. dubliniensis by germ tube production, biochemical test (Candida identification kit), CHROM agar Candida differential medium and growth at 45°C. Virulence factors of the C. dubliniensis, viz., phospholipase, proteinase, esterase, lipase, hemolysin, and biofilm production were detected by standard procedures.Results: Maximum number of C. dubliniensis have ability to produce protienase 6 (66.66%), followed by esterase 5 (55.55%), biofilm formation 4 (44.44%), hemolysin 2 (22.22%), and phospholipase and lipase 1 (11.1%), respectively. Conclusion:The result revealed that these enzymes are potential virulence factors are the most important thing to realize pathogenesis of C. dubliniensis.

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“…However, despite the close phylogenetic relationship between C. albicans and C. dubliniensis, phenotypic and molecular data suggest differences in virulence between these two species. The apparent low virulence of C. dubliniensis could be due to differential gene expression rather than to simple absence or divergence of particular genes [4] . The study of Saccharomyces cerevisiae has been important in the elucidation of proteinase multiplicity and proteinase function in eukaryotic cells, numerous proteolytic enzymes have been biochemically and genetically characterized [5] .…”

Section: Introductionmentioning

confidence: 99%

The Proteolytic System of Candida dubliniensis

Salomé¹,

Parra-Ortega²,

Bautista-Muñoz³

et al. 2007

American J. of Infectious Diseases

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Proteases of Candida dubliniensis have been scarcely studied, these enzymes may play an important role in nitrogen metabolism, post-translational processing, nutritional stress, dimorphism, virulence, etc. In this work, we report the presence of five different intracellular proteases and one extracellular proteolytic activity. The intracellular proteases are: aminopeptidase ycdAPE, carboxypeptidase ycdCP, dipeptidyl aminopeptidase ycdDAP, proteinases ycdPrA and ycdPrB, and extracellular protease Sap activity, measured under several nutritional conditions. C. dubliniensis produced the highest level of intracellular proteolytic enzymes, i.e., ycdAPE, ycdCP, ycdDAP, ycdPrA and ycdPrB in media with peptone during stationary growth phase. Chelating agents affected mainly APE activity; whereas ycdCp, ycdDAP, and ycdPrB were affected by serine protease inhibitors; ycdPrA was affected by pepstatin, an aspartyl protease inhibitor. We found Sap activity in C. dubliniensis in YCB-SBA medium, this activity was inhibited by pepstatin inhibitor. Southern analysis revealed the presence of at least four genes encoding Sap in the C. dublinienisis genome (using as probes SAP1, SAP2, SAP3, and SAP4-6 genes from C. albicans).

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Candida dubliniensis: Ten years on

Cited by 112 publications

References 67 publications

Evaluation of Pyrosequencing® technology for the identification of clinically relevant non-dematiaceous yeasts and related species

Evaluation of Pyrosequencing® technology for the identification of clinically relevant non-dematiaceous yeasts and related species

Incidence and Virulence Traits of Candida Dubliniensis Isolated From Clinically Suspected Patients

The Proteolytic System of Candida dubliniensis

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