Candida Biofilms on Oral Biomaterials

Courtois, Philippe

doi:10.5772/24338

Cited by 3 publications

(2 citation statements)

References 63 publications

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“…Candida albicans ( C. albicans ), a commensal yeast in the oral cavity, can incorporate into biofilms that form on denture surfaces (1,2), leading in some cases to mucosal infections referred to as denture stomatitis (3–5). …”

Section: Introductionmentioning

confidence: 99%

Dose-dependent effect of lysozyme upon Candida albicans biofilm

Sebaa

Hizette

Boucherit-Otmani

et al. 2017

Molecular Medicine Reports

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The present study investigated the in vitro effect of lysozyme (0–1,000 µg/ml) on Candida albicans (C. albicans) biofilm development. Investigations were conducted on C. albicans ATCC 10231 and on 10 clinical isolates from dentures. Strains were cultured aerobically at 37°C in Sabouraud broth. Yeast growth was evaluated by turbidimetry. Biofilm biomass was quantified on a polystyrene support by crystal violet staining and on acrylic surfaces by counts of colony forming units. Lysozyme affected biofilm formation to a greater extent than it affected growth. For the ATCC 10231 reference strain, lysozyme acted as a biofilm promotor on polystyrene at the highest concentration tested (1,000 µg/ml, non-physiological). When the reference strain was investigated on acrylic resin support, lysozyme acted as a significant biofilm promotor on rough resin, but less on smooth resin. The attached biomass in the presence of physiological concentrations of lysozyme (10–30 µg/ml) was significantly decreased compared with the hypothetical value of 100% using a one-sample t-test, but a comparison between the different lysozyme conditions using analysis of variance and post hoc tests did not reveal significant differences. In 10 wild strains, different patterns of biofilm formation on polystyrene were observed in the presence of lysozyme. Some strains, characterized by large amounts of biofilm formation in the presence of 1,000 µg/ml lysozyme, were poor biofilm producers at low concentrations of lysozyme. In contrast, some strains that were poor biofilm producers with a high lysozyme concentration were more inhibited by low concentrations of lysozyme. The present study emphasizes the need to develop strategies for biofilm control based on in vitro experiments, and to implement these in clinical trials prior to approval of hygiene products enriched with exocrine proteins, such as lysozyme. Further studies will extend these investigations to other Candida species, and to fungi and bacteria present in oral biofilms.

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Section: Introductionmentioning

confidence: 99%

Dose-dependent effect of lysozyme upon Candida albicans biofilm

Sebaa

Hizette

Boucherit-Otmani

et al. 2017

Molecular Medicine Reports

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“…The most commonly isolated species are C. albicans (78%), C. glabrata (44%), and C. tropicalis (19%). 15 Another study had also mentioned that Candida adhesion is a complex multifactorial process because it depended on the host and characteristics of each Candida.…”

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Comparison of Candida sp. Colonies in Gargling-volume Culture from Subject Wearers of Heat-cured and Selfcured Acrylic Resin Removable Partial Dentures

Rahmayani¹,

Mubarak²,

Nasution³

et al. 2017

World Journal of Dentistry

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Antimicrobial enzymes: An emerging strategy to fight microbes and microbial biofilms

Thallinger¹,

Prasetyo²,

Nyanhongo³

et al. 2013

Biotechnology Journal

260

198

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With the increasing prevalence of antibiotic resistance, antimicrobial enzymes aimed at the disruption of bacterial cellular machinery and biofilm formation are under intense investigation. Several enzyme-based products have already been commercialized for application in the healthcare, food and biomedical industries. Successful removal of complex biofilms requires the use of multi-enzyme formulations that contain enzymes capable of degrading microbial DNA, polysaccharides, proteins and quorum-sensing molecules. The inclusion of anti-quorum sensing enzymes prevents biofilm reformation. The development of effective complex enzyme formulations is urgently needed to deal with the problems associated with biofilm formation in manufacturing, environmental protection and healthcare settings. Nevertheless, advances in synthetic biology, enzyme engineering and whole DNA-Sequencing technologies show great potential to facilitate the development of more effective antimicrobial and anti-biofilm enzymes.

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Candida Biofilms on Oral Biomaterials

Cited by 3 publications

References 63 publications

Dose-dependent effect of lysozyme upon Candida albicans biofilm

Dose-dependent effect of lysozyme upon Candida albicans biofilm

Comparison of Candida sp. Colonies in Gargling-volume Culture from Subject Wearers of Heat-cured and Selfcured Acrylic Resin Removable Partial Dentures

Antimicrobial enzymes: An emerging strategy to fight microbes and microbial biofilms

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