Cancer stem cell marker expression and methylation status in patients with colorectal cancer

Meršaková, Sandra; Janíková, Katarína; Kalman, Michal; Marcinek, Juraj; Grendár, Marian; Vojtko, Martin; Kycina, Roman; Pindura, Miroslav; Janík, Ján; Mikolajcik, Peter; Laca, Ľ; Mejstříková, Ester; Halašová, Erika; Strnádel, Ján; Lasabová, Zora

doi:10.3892/ol.2022.13352

Cited by 4 publications

(3 citation statements)

References 55 publications

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“…The number of individuals diagnosed with colorectal cancer (CRC) has been on an alarming upward trajectory over the past decade. In some countries, this cancer represents one of the most frequently diagnosed types of neoplasia (4). It had been estimated that in 2020, the incidence and mortality rates of colorectal cancer would increase by more than 1.9 million cases and 930,000 deaths, respectively.…”

Section: Introductionmentioning

confidence: 99%

Joinpoint analysis of colorectal cancer trend in the Slovakia

PHAM,

PEKARCIKOVA,

EDELSTEIN

et al. 2023

BLL

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OBJECTIVES: The aim of this study is to describe the colorectal cancer trend in the Slovakia between 2002 and 2019. BACKGROUND: In 2020, the Slovakia ranked second among the 10 countries with the highest incidence of colorectal cancer and the highest number of deaths from colorectal cancer (hereafter also referred to as CRC). METHODS: To describe the situation of CRC, indicators of incidence and mortality rates stratifi ed by age and sex for the available time period were chosen. A joinpoint regression software was used to identify changes in the trend of development. RESULTS: During the 18-year follow-up period (2002)(2003)(2004)(2005)(2006)(2007)(2008)(2009)(2010)(2011)(2012)(2013)(2014)(2015)(2016)(2017)(2018)(2019), the overall trend in colorectal cancer incidence continued to increase with an overall mean annual change of 1.3 %. The incidence of CRC tended to increase from 50 years of age and increased with age. The most pronounced increasing trend was observed in the age group of 75 years and older (AAPC in men 1.9 %, IS +1.4; +2.5 and in women 2.0 %, IS +1.6; +2.4). CRC mortality remained relatively stable for the entire 18-year period. A decreasing trend in mortality was observed in the 25-49 age group with an overall annual percentage decrease of 0.9 % (IS -1.5; -0.3), while an increasing trend was observed in the 75+ age group with an overall annual percentage increase of 1.0 % (IS +0.8; +1.3). The incidence and mortality rates in men were higher than in women. CONCLUSION: The situation of colorectal cancer trend in the Slovakia has improved compared to the previous period (1971-2001).

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Section: Introductionmentioning

confidence: 99%

Joinpoint analysis of colorectal cancer trend in the Slovakia

PHAM,

PEKARCIKOVA,

EDELSTEIN

et al. 2023

BLL

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“…A disintegrin and metalloproteinase domain with thrombospondin motifs 16 (ADAMTS16) belongs to the ADAMTS family. Its role has been investigated in esophageal squamous cell carcinoma ( 14 ) and CRC ( 15 , 16 ). However, to the best of our knowledge, the role of ADAMTS16 in HCC has not yet been studied.…”

Section: Introductionmentioning

confidence: 99%

Association between the expression levels of ADAMTS16 and BMP2 and tumor budding in hepatocellular carcinoma

Jiang

Zhang

et al. 2023

Oncol Lett

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Tumor budding (TB) has become a crucial factor for predicting the malignancy grade and prognostic outcome for multiple types of solid cancer. Studies have investigated the prognostic value of TB in hepatocellular carcinoma (HCC). However, its molecular mechanism in HCC remains unclear. To the best of our knowledge, the present study was the first to compare the expression of differentially expressed genes (DEGs) between TB-positive (TB-pos) and TB-negative HCC tissues. In the present study, total RNA was extracted from 40 HCC tissue specimens and then sequenced. According to Gene Ontology (GO) functional annotation, upregulated DEGs were markedly associated with embryonic kidney development-related GO terms, which suggested that the TB process may at least partly mimic the process of embryonic kidney development. Subsequently, two genes, a disintegrin and metalloproteinase domain with thrombospondin motifs 16 (ADAMTS16) and bone morphogenetic protein 2 (BMP2), were screened and verified through immunohistochemical analysis of HCC tissue microarrays. According to the immunohistochemical results, ADAMTS16 and BMP2 were upregulated in TB-pos HCC samples, and BMP2 expression was increased in budding cells compared with the tumor center. Additionally, through cell culture experiments, it was demonstrated that ADAMTS16 and BMP2 may promote TB of liver cancer, thus promoting the malignant progression of liver cancer. Further analysis revealed that ADAMTS16 expression was associated with necrosis and cholestasis, and BMP2 expression was associated with the Barcelona Clinic Liver Cancer stage and the vessels encapsulating tumor clusters. Overall, the findings of the present study provided insights into the possible mechanisms of TB in HCC and revealed potential anti-HCC therapeutic targets.

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“…Cakmak et al reported that ADAMTS16 was downregulated in a chondrosarcoma cell [ 11 ]. In colorectal cancer (CRC), ADAMTS16 was hypermethylated and high expression of ADAMTS16 restrained cancer cell proliferation [ 9 , 13 ]. Meanwhile, the mutation of ADAMTS16 altered the sensibility of ovarian cancer to platinum-based chemotherapy [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

The Novel Protein ADAMTS16 Promotes Gastric Carcinogenesis by Targeting IFI27 through the NF-κb Signaling Pathway

Zhou

Jiang

et al. 2022

IJMS

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A disintegrin and metalloproteinase with thrombospondin motifs 16 (ADAMTS16) has been reported to be involved in the pathogenesis of solid cancers. However, its role in gastric cancer (GC) is unclear. In this study, the role of ADAMTS16 in gastric cancer was investigated. The effects of ADAMTS16 on cell migration, invasion, and proliferation were investigated by functional experiments in vivo and in vitro. Downstream signal pathways of ADAMTS16 were confirmed by using bioinformatics analysis, co-immunoprecipitation, and immunofluorescence. Meanwhile, bioinformatics analysis, qRT-PCR, western blot, and dual-luciferase reporter gene analysis assays were used to identify ADAMTS16 targets. The expression of ADAMTS16 in GC was analyzed in public datasets. The expression of ADAMTS16 and its correlations with the clinical characteristics of GC were investigated by immunohistochemistry. Ectopic ADAMTS16 expression significantly promoted tumor cell migration, invasion, and growth. Bioinformatics analysis and western blot showed that ADAMTS16 upregulated the IFI27 protein through the NF-κb pathway, which was confirmed by immunofluorescence and western blot. Dual-luciferase reporter gene analysis identified a binding site between P65 and IFI27 that may be directly involved in the transcriptional regulation of IFI27. IFI27 knockdown reversed the promoting effect of ADAMTS16 on cell invasion, migration, and proliferation indicating that ADAMTS16 acts on GC cells by targeting the NF-κb/IFI27 axis. ADAMTS16 was associated with poor prognosis in clinical characteristics. ADAMTS16 promotes cell migration, invasion, and proliferation by targeting IFI27 through the NF-κB pathway and is a potential progressive and survival biomarker of GC.

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Cancer stem cell marker expression and methylation status in patients with colorectal cancer

Cited by 4 publications

References 55 publications

Joinpoint analysis of colorectal cancer trend in the Slovakia

Joinpoint analysis of colorectal cancer trend in the Slovakia

Association between the expression levels of ADAMTS16 and BMP2 and tumor budding in hepatocellular carcinoma

The Novel Protein ADAMTS16 Promotes Gastric Carcinogenesis by Targeting IFI27 through the NF-κb Signaling Pathway

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