Cancer diagnosis and immunotherapy in the age of CRISPR

Cook, Peter J.; Ventura, Andrea

doi:10.1002/gcc.22702

Cited by 4 publications

(4 citation statements)

References 124 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To demonstrate the potential application value of RCH, it was applied to detect circulating let-7a in serum samples from patients with non-small cell lung cancer (NSCLC) and healthy volunteers. As it was reported that since the expression of circulating let-7a was significantly down-regulated in patients with NSCLC, it could become a useful biomarker for NSCLC[ 41 ]. It was also discovered that the detection results were highly consistent with RT-PCR results and the literature reports, suggesting that this method could be used for the screening and diagnosis of tumors in the near future.…”

Section: Clinical Application Of Crispr/cas Technologymentioning

confidence: 99%

“…While the SHERLOCK and SHERLOCKv2 molecular detection platforms can detect the BRAF V600E mutation in simulated circulating DNA samples and the EGFR L858R mutation in liquid biopsy samples from adenocarcinoma patients[ 26 , 29 , 43 ], the DETECTR method can also be used to rapidly detect tumor mutations in the reproductive system[ 30 ]. For example, human papillomavirus, closely related to the occurrence of cervical cancer, is detected in human anal wipe DNA extracts[ 41 ]. The development of this technology is expected to bring a significant breakthrough in the early screening of cervical cancer (Table 3 ).…”

Section: Clinical Application Of Crispr/cas Technologymentioning

confidence: 99%

See 1 more Smart Citation

Development of clustered regularly interspaced short palindromic repeats/CRISPR-associated technology for potential clinical applications

Huang¹,

Zhang²,

Zhu³

et al. 2022

WJCC

View full text Add to dashboard Cite

The clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated (Cas) proteins constitute the innate adaptive immune system in several bacteria and archaea. This immune system helps them in resisting the invasion of phages and foreign DNA by providing sequence-specific acquired immunity. Owing to the numerous advantages such as ease of use, low cost, high efficiency, good accuracy, and a diverse range of applications, the CRISPR-Cas system has become the most widely used genome editing technology. Hence, the advent of the CRISPR/Cas technology highlights a tremendous potential in clinical diagnosis and could become a powerful asset for modern medicine. This study reviews the recently reported application platforms for screening, diagnosis, and treatment of different diseases based on CRISPR/Cas systems. The limitations, current challenges, and future prospectus are summarized; this article would be a valuable reference for future genome-editing practices.

show abstract

Section: Clinical Application Of Crispr/cas Technologymentioning

confidence: 99%

Section: Clinical Application Of Crispr/cas Technologymentioning

confidence: 99%

Development of clustered regularly interspaced short palindromic repeats/CRISPR-associated technology for potential clinical applications

Huang¹,

Zhang²,

Zhu³

et al. 2022

WJCC

View full text Add to dashboard Cite

show abstract

“…Secondly, although various approaches have been reported to improve gRNA design ( Bin Moon et al, 2018 ; Matson et al, 2019 ; Han et al, 2020 ) and increase the specificity of Cas enzyme ( Kleinstiver et al, 2016 ; Slaymaker et al, 2016 ), the risk of off-target effects, resulting from non-specific cutting and further leading to unwanted mutations, still remains a major obstacle to translation of CRISPR/Cas9 system to clinical therapeutic use ( Cook and Ventura, 2019 ). Whereas, in clinical trials, no significant or only a small number of off-target sites, chromosomal rearrangements or long-range deletions were actually detected.…”

Section: Perspectives and Challengesmentioning

confidence: 99%

CRISPR/Cas9 Gene-Editing in Cancer Immunotherapy: Promoting the Present Revolution in Cancer Therapy and Exploring More

Yin

et al. 2021

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

In recent years, immunotherapy has showed fantastic promise in pioneering and accelerating the field of cancer therapy and embraces unprecedented breakthroughs in clinical practice. The clustered regularly interspaced short palindromic repeat (CRISPR)-associated protein 9 (CRISPR-Cas9) system, as a versatile gene-editing technology, lays a robust foundation to efficiently innovate cancer research and cancer therapy. Here, we summarize recent approaches based on CRISPR/Cas9 system for construction of chimeric antigen receptor T (CAR-T) cells and T cell receptor T (TCR-T) cells. Besides, we review the applications of CRISPR/Cas9 in inhibiting immune checkpoint signaling pathways and highlight the feasibility of CRISPR/Cas9 based engineering strategies to screen novel cancer immunotherapy targets. Conclusively, we discuss the perspectives, potential challenges and possible solutions in this vivid growing field.

show abstract

“…So, in designing gRNAs, genetic variabilities among individuals should be taken into consideration. 50 …”

Section: Crispr/cas Systemmentioning

confidence: 99%

CRISPER/CAS System, a Novel Tool of Targeted Therapy of Drug-resistant Lung Cancer

et al. 2021

View full text Add to dashboard Cite

Lung cancer (LC) is the most common cause of cancer-related death worldwide. Patients with LC are usually diagnosed at advanced phases. Five-year survival rate in LC patients is approximately 16%. Despite decades of research on LC treatments, clinical outcomes are still very poor, necessitating to develop novel technologies to manage the disease. Considering the role of genetic and epigenetic changes in oncogenes and tumor-suppressor genes in cancer progression, gene therapy provides a hot spot in cancer treatment research. Gene therapy offers less side effects compared to conventional methods such as chemotherapy. Unlike the traditional approaches of gene therapy that have temporary effects, using genetic modification tools can offer persistent cure. Over the past a few years, many studies have effectively used the CRISPR–Cas9 approach to modify gene expression in cells. This system is applied to induce site-specific mutagenesis and epigenetic modifications and regulate gene expression. In this review, we discuss recent applications of the CRISPR–Cas9 technology in treating LC.

show abstract

Cancer diagnosis and immunotherapy in the age of CRISPR

Cited by 4 publications

References 124 publications

Development of clustered regularly interspaced short palindromic repeats/CRISPR-associated technology for potential clinical applications

Development of clustered regularly interspaced short palindromic repeats/CRISPR-associated technology for potential clinical applications

CRISPR/Cas9 Gene-Editing in Cancer Immunotherapy: Promoting the Present Revolution in Cancer Therapy and Exploring More

CRISPER/CAS System, a Novel Tool of Targeted Therapy of Drug-resistant Lung Cancer

Contact Info

Product

Resources

About