Development of clustered regularly interspaced short palindromic repeats/CRISPR-associated technology for potential clinical applications

Abstract

The clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated (Cas) proteins constitute the innate adaptive immune system in several bacteria and archaea. This immune system helps them in resisting the invasion of phages and foreign DNA by providing sequence-specific acquired immunity. Owing to the numerous advantages such as ease of use, low cost, high efficiency, good accuracy, and a diverse range of applications, the CRISPR-Cas system has become the most widely used genome editing technology. Hence, the advent of the CRISPR/Cas technology highlights a tremendous potential in clinical diagnosis and could become a powerful asset for modern medicine. This study reviews the recently reported application platforms for screening, diagnosis, and treatment of different diseases based on CRISPR/Cas systems. The limitations, current challenges, and future prospectus are summarized; this article would be a valuable reference for future genome-editing practices.

Human Brain Organoids in Migraine Research: Pathogenesis and Drug Development

“…This technology enabled disease modeling toward precision medicine [ 21 ]. The development of clustered, regulatory, interspaced, short, palindromic repeat (CRISPR)/Cas9 endonucleases has enabled the creation of genetically edited hiPSC-based disease models [ 22 , 23 ]. However, iPSC-derived 2D cell cultures have some limitations compared with 3D cell cultures [ 24 ].…”

Untitled

“…[12] Bacteria would become re-sensitive to common antibiotics if these resistancemediating plasmids were removed from them in a systematic manner. [13] Plasmids, thus, present unique targets that have not yet been used clinically. [14] ORIGINAL ARTICLE…”