Cancer chemopreventive activity of the prenylated coumarin, umbelliprenin, in vivo

Iranshahi, Mehrdad; Sahebkar, Amirhossein; Takasaki, Midori; Konoshima, Takao; Tokuda, Harukuni

doi:10.1097/cej.0b013e32832c389e

Cited by 149 publications

(70 citation statements)

References 29 publications

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“…Recently, there is a growing interest in the use of natural products for anticancer activity against cancer development (Ben Ammar et al 2009;KilaniJaziri et al 2009;Ben Sghaier et al 2011). These include fatty acids, vitamins, minerals, lichens, flavonoids and terpenoids (Chaudhary et al 2009;Iranshahi et al 2009;Ben Sghaier et al 2011;Ranković et al 2011). Terpenoids are one of the most important substances of this which are naturally occurring in plants that are thought to have positive effects on human and animal health (Hamułka et al 2012;Styrczewska et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Cytotoxicity and genotoxicity of zingiberene on different neuron cell lines in vitro

et al. 2014

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The main objective of this study is to investigate the cytotoxic, genotoxic and antioxidant properties of zingiberene (ZBN) in an in vitro rat brain cell culture study. The cytotoxic effect was determined against the rat neuron and N2a neuroblastoma (N2a-NB) cell lines using the 3,(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, while the antioxidant activity was assessed using the total antioxidant capacity (TAC) and total oxidative stress (TOS) assays. The effects on DNA damage were also evaluated in this study by the single cell gel electrophoresis assay. The results indicated that ZBN has an anti-proliferative activity suppressing the proliferation of N2a-NB cells at concentrations over 50 mg L -1 and neuron cells at concentrations over 150 mg L -1. In addition, ZBN treatments at higher doses (B50 mg L -1 ) led to increases of TOS levels in N2a-NB cell cultures. However 25 mg L -1 of ZBN treatment caused increases of TAC levels in cultured neuron and N2a-NB cell cultures while ZBN at doses of 10-400 mg L -1 did not increase the number of total damage score in both cell lines. This study clearly indicates that ZBN has a significant potential to be used as a natural anticancer agent in cultured N2a-NBs.

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Section: Introductionmentioning

confidence: 99%

Cytotoxicity and genotoxicity of zingiberene on different neuron cell lines in vitro

et al. 2014

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“…The plant compounds are widely used in treating cancer. Some of these compounds show anti tumor activity (Iranshahi et al, 2009;Sahebkar and Iranshahi, 2010). Bracken fern [Pteridium aquilinum (L.) kuhn (Dennstaedtiaceae)] as one of the most popular herbs on the planet (Taylor, 1990), is believed to carry such properties.…”

Section: Introductionmentioning

confidence: 99%

Bracken-fern Extracts Induce Cell Cycle Arrest and Apoptosis in Certain Cancer Cell Lines

Roudsari

Bahrami²,

Dehghani³

et al. 2012

Asian Pacific Journal of Cancer Prevention

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Bracken fern [Pteridium aquilinem (L.) kuhn (Dennstaedtiaceae)] is one of the most common species on the planet. It has been consumed by humans and animals for centuries. Use by some human groups is because they believe bracken fern is good for health as plant medicine. However, it is also one of the few known plants that can cause tumors in farm animals. Many interested groups have focused their attention on bracken fern because of these interesting features. In order to evaluate the biological effects of exposure to this plant in cellular level, human cancer cell lines were treated with the fern dichloromethane extracts and the genotoxic and cytotoxic effects were studied. Anti-proliferative/cytotoxic effects were evaluated by cell count, MTT assay and flow cytometry methods with three different cancer cell lines, TCC, NTERA2, and MCF-7, and two normal cells, HDF1 and HFF3. Pro-apoptotic effects of the extracts were determined by DAPI staining and comet assay, on TCC cancer cells compared to the normal control cell lines. Cellular morphology was examined by light microscopy. Our present study showed that the extract caused DNA damage and apoptosis at high concentrations (200 µg/mL) and also it may induce cell cycle arrest (G2/M phase) at mild concentrations (50 and 30 µg/mL) depending on the cell type and tumor origin. These results indicate that bracken fern extract is a potent source of anticancer compounds that could be utilized pharmaceutically.

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“…Despite the dominant cytotoxic activities of Umb against certain cancer cell lines (2,3,8), its proliferative activities have been observed in normal lymphocytes (2) and at lower doses in a colorectal cancer cell line in culture media (11), indicating that this compound may have different levels of cytotoxicity against different cancer cell lines in vitro.…”

Section: Introductionmentioning

confidence: 99%

“…This compound is able to suppress tumorigenesis through its anti-inflammatory, anti-genotoxicity, anti-invasive and lipoxygenase inhibitory activities (3)(4)(5)(6). In vitro, Umb has been reported to attenuate the proliferation of various cancer cell lines, and is able to prevent/delay cancer in in vivo animal models of papilloma and lung cancer (7)(8)(9). It hasbeen stated that the anti-tumor activities of Umb are partly due to a prenyl moiety (C7-OH) on the structure of the umbelliferone nucleus (3).…”

Section: Introductionmentioning

confidence: 99%

Immune-associated proteins with potential in vivo anti-tumor activities are upregulated in lung cancer cells treated with umbelliprenin: A proteomic approach

et al. 2016

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Abstract. Umbelliprenin (Umb), a natural coumarin, has demonstrated anti-tumor activities, both in vitro and particularly in vivo, in several types of cancer, including lung cancer. The present study aimed to identify molecular targets of Umb using a high-throughput approach. Lung cancer cell lines, QU-DB (large-cell lung carcinoma) and A549 (adenocarcinoma), were treated with Umb. Differentially-expressed proteins were identified using two-dimensional electrophoresis coupled to mass spectrometry. In the QU-DB cells, differential expression of proteins, including downregulation of the tumorigenic protein heat shock protein 90 kDa and upregulation of the potential anti-tumor proteins Nipsnap1 and glycine-tRNA ligase (GRS), suggested that Umb is a strong anti-tumor compound. In the A549 cells, differential expression of proteins indicated possible contradictory effects of Umbregarding tumorigenesis, which included downregulation of the tumorigenic protein cyclophilin and the tumor suppressor MST, and upregulation of stathmin (tumorigenic) and calreticulin. Calreticulun, in addition to GRS in QU-DB cells, stimulates anti-tumor immune responses in vivo. To the best of our knowledge, the present study is the first to use a high-throughput approach to identify targets of Umb in cancer. These molecular targets suggested that Umb may exhibit stronger in vitro anti-tumor activity against the large-cell carcinoma model than the adenocarcinoma model. Furthermore, it has been reported that Umb exhibits higher cytotoxicity against QU-DB cells than A549 cells in vitro, and significant Umb anti-tumor activity against lung cancer in vivo, which is consistent with previously published literature. In each cell type, immune-associated molecules were upregulated, indicating that this naturally occurring compound exhibits marked anti-tumor activity in vivo. However, further studies that investigate the effect of Umb in different in vitro models of cancer are required.

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Cancer chemopreventive activity of the prenylated coumarin, umbelliprenin, in vivo

Cited by 149 publications

References 29 publications

Cytotoxicity and genotoxicity of zingiberene on different neuron cell lines in vitro

Cytotoxicity and genotoxicity of zingiberene on different neuron cell lines in vitro

Bracken-fern Extracts Induce Cell Cycle Arrest and Apoptosis in Certain Cancer Cell Lines

Immune-associated proteins with potential in vivo anti-tumor activities are upregulated in lung cancer cells treated with umbelliprenin: A proteomic approach

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