Cancer Cell Membrane Biomimetic Mesoporous Nanozyme System with Efficient ROS Generation for Antitumor Chemoresistance

Abstract

Single-atom nanozymes (SAZs) with reaction specificity and optimized catalytic properties have great application prospects in tumor therapy. But the complex tumor microenvironment (low content of H2O2) limits its therapeutic effect. In this study, we developed a bionic mesoporous Fe SAZs/DDP nanosystem (CSD) for enhanced nanocatalytic therapy (NCT)/chemotherapy by simultaneously encapsulating the chemotherapeutic drugs cisplatin (DDP) and Fe SAZs with high peroxidase (POD) activity into the cancer cell membrane. CSD could evade immune recognition and actively targets tumor sites, and DDP upregulates endogenous H2O2 levels by activating nicotinamide adenine dinucleotide phosphate (NADPH) oxidase, thereby enhancing SAZs-mediated hydroxyl radical (·OH) production, which subsequently leads to mitochondrial damage and intolerance to chemotherapy drug. We used the HGC27/DDP cell line for in vitro and in vivo experiments. The results showed that CSD achieved good therapeutic benefits, without any side effects such as inflammatory reaction. This system can induce multiple antitumor effect with H2O2 self-supply, mitochondrial damage, and ATP downregulation and eventually lead to chemosensitization.

Atomic Engineering of Single‐Atom Nanozymes for Biomedical Applications

“…This, in turn, induced mitochondrial damage, reduced adenosine triphosphate (ATP) content, and further improved the therapeutic outcome (Figure 12a). [91] The cytotoxicity was evaluated in Figure 12b, the CSD group achieved optimal tumor cell inhibition effect.…”

“…Figure 12h represents the in vivo tumor suppression of this system that remained stable over 26 days. Next, to further monitor the status of tumor cells, staining was performed with [91] Copyright 2022, Hindawi. e) A description of the nano catalytic tumor therapy in the TME cascade reaction of Co SAzyme; f) Cytotoxicity tests after 24 h incubation with MDA-MB-231 cells and Co-SAs@NC at pH 7.4 or pH 6.0 medium (n = 5); g) Fluorescence imaging of intracellular ROS levels after 4 h incubation at Co-SAs@NC in cell media at pH 7.4 or pH 6.0; h) Tumor growth curves after treatment 26 days; i) Live and dead staining imaging of MDA-MB-231 cells incubation with NC or Co-SAs@NC after 24 h in cell culture medium at pH 6.0.…”

Nanozyme-Based Remodeling of Disease Microenvironments for Disease Prevention and Treatment: A Review

“…Wang et al used tumor CM to coat Au–Rh nanostructures, and the CM-coated NPs could significantly target tumor sites when compared to the untreated group ( p < 0.01). The use of tumor CM to coat nanozymes can evade immune recognition while actively targeting tumor sites. , …”

Novel Immunosensor Based on Metal Single-Atom Nanozymes with Enhanced Oxidase-Like Activity for Capsaicin Analysis in Spicy Food

“…Recently, there has been increased interest and concern in catalysis-related fields regarding single-atom nanozyme (SAzyme) applications based on iron (Fe). This heightened attention is driven by their precise and defined electronic and geometric structures, distinctive quantum size effects, and exceptional atomic use efficiency. − Moreover, single-atom active isolated metal nanozymes have been shown to be firmly anchored in support cavities and accompanied by superior stability . Importantly, SAzymes can be oxidized by molecular oxygen in the air without expendable H 2 O 2 , which may eliminate interference from unstable H 2 O 2 and redundant operations.…”