Can we truly rely on the urinary antigen test for the diagnosis? Legionella case report

Miyata, Jun; Huh, Ji Young; Ito, Yukiko; Kobuchi, Taketsune; Kusukawa, Kazuko; Hayashi, Hiroyuki

doi:10.1002/jgf2.16

Cited by 4 publications

(1 citation statement)

References 25 publications

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“…Part of the explanation for this lies in the detection method used and the designation of confirmed cases. A urinary antigen test is often used to diagnose legionellosis, but it is not sensitive enough [ 35 ]. Although not time-consuming and easily implemented, this test only detects Legionella pneumophila serotype 1 ( Lp1 ).…”

Section: Introductionmentioning

confidence: 99%

Intracellular Behaviour of Legionella Non-pneumophila Strains within Three Amoeba Strains, Including Willaertia magna C2c Maky

Croze¹,

Carlino²,

Quélard³

et al. 2021

Pathogens

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Legionellosis, an often-lethal pneumonia, is generally associated with contamination by Legionella pneumophila. This bacterium can persist in the environment and resist chemical treatment when it is internalized by amoebae. In addition, there is increasing medical proof that other Legionella species can be causative agents of Legionellosis. The objective of this study was to evaluate whether Legionella non-pneumophila (Lnp) strains were able to use the machinery of amoeba to multiply, or whether amoebae were able to limit their proliferation. Seven strains belonging to the species L. longbeachae, L. anisa, L. bozemanae, L. taurinensis, and L. dumoffii were cocultured with three amoebae, Acanthamoeba castellanii, Willaertia magna T5(S)44, and Willaertia magna C2c Maky, at two temperatures, 22 and 37 °C. We found that at 22 °C, all amoebae were able to phagocytose the seven Lnp strains and to avoid intracellular development, except for L. longbeachae, which was able to multiply inside W. magna T5(S)44. At 37 °C, four Lnp strains were able to hijack the machinery of one or two amoebae and to use it to proliferate, but none were able to multiply inside W. magna C2c Maky.

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Section: Introductionmentioning

confidence: 99%