Intracellular Behaviour of Legionella Non-pneumophila Strains within Three Amoeba Strains, Including Willaertia magna C2c Maky

Croze, Aurélien; Carlino, Antoine; Quélard, Benjamin; Saha, Adeline; Convert, Tiphaine; Eberst, Jean-Baptiste; Troussieux, Sandrine

doi:10.3390/pathogens10101350

Cited by 7 publications

(5 citation statements)

References 59 publications

(81 reference statements)

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“…This species is associated with cases of legionellosis. We can surmise that 6 out of 15 Legionella species having this protein are human pathogens ( L. parisiensis, bozemanae, jamestowniensis, tucsonensis, jordanis and anisa ) 1 , 48 , 49 .…”

Section: Resultsmentioning

confidence: 99%

Pan-kinome of Legionella expanded by a bioinformatics survey

Krysińska

Baranowski

Deszcz

et al. 2022

Sci Rep

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The pathogenic Legionella bacteria are notorious for delivering numerous effector proteins into the host cell with the aim of disturbing and hijacking cellular processes for their benefit. Despite intensive studies, many effectors remain uncharacterized. Motivated by the richness of Legionella effector repertoires and their oftentimes atypical biochemistry, also by several known atypical Legionella effector kinases and pseudokinases discovered recently, we undertook an in silico survey and exploration of the pan-kinome of the Legionella genus, i.e., the union of the kinomes of individual species. In this study, we discovered 13 novel (pseudo)kinase families (all are potential effectors) with the use of non-standard bioinformatic approaches. Together with 16 known families, we present a catalog of effector and non-effector protein kinase-like families within Legionella, available at http://bioinfo.sggw.edu.pl/kintaro/. We analyze and discuss the likely functional roles of the novel predicted kinases. Notably, some of the kinase families are also present in other bacterial taxa, including other pathogens, often phylogenetically very distant from Legionella. This work highlights Nature’s ingeniousness in the pathogen–host arms race and offers a useful resource for the study of infection mechanisms.

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Section: Resultsmentioning

confidence: 99%

Pan-kinome of Legionella expanded by a bioinformatics survey

Krysińska

Baranowski

Deszcz

et al. 2022

Sci Rep

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“…The use of protists as microbial inoculum has not been widely adopted in the agricultural market. Nevertheless, the first protist‐based BS and BCA products have been or are in the process of being established in the market (e.g., Ecostyle ProtoPlus®, Amoéba W. magna C2c Maky; Croze et al, 2021). Currently available nematode‐based BCA products, however, focus entirely on entomopathogenic nematodes that control insect pests independent of the soil microbiome (Abd‐Elgawad, 2019; Nxitywa & Malan, 2022; Shapiro‐Ilan et al, 2023).…”

Section: Are Microbiome Predators Applicable Such As In Agriculture A...mentioning

confidence: 99%

Microbiome predators in changing soils

Hu,

Li,

Berlinches de Gea

et al. 2023

Environmental Microbiology

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Microbiome predators shape the soil microbiome and thereby soil functions. However, this knowledge has been obtained from small‐scale observations in fundamental rather than applied settings and has focused on a few species under ambient conditions. Therefore, there are several unaddressed questions on soil microbiome predators: (1) What is the role of microbiome predators in soil functioning? (2) How does global change affect microbiome predators and their functions? (3) How can microbiome predators be applied in agriculture? We show that there is sufficient evidence for the vital role of microbiome predators in soils and stress that global changes impact their functions, something that urgently needs to be addressed to better understand soil functioning as a whole. We are convinced that there is a potential for the application of microbiome predators in agricultural settings, as they may help to sustainably increase plant growth. Therefore, we plea for more applied research on microbiome predators.

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“…This was considered as the initial incubation time (time zero). At certain points in time, after infection (0, 2, 4, 6, 8, and 10 h), the amoeba cells were treated with 0.1% Triton X-100 (Sigma, St. Louis, MO, USA [33]) for 10 min to lyse the amoeba cells. The number of bacteria cultured in amoeba was determined by the CFU method, by adding serially diluted suspensions of Legionella on BCYE agar plates, and incubation for 72 h at 37 • C. The bacteria cultured in amoeba for 4 days were used in pasteurization trials.…”

Section: Infection Of Amoeba With L Pneumophilamentioning

confidence: 99%

Decreasing Pasteurization Treatment Efficiency against Amoeba-Grown Legionella pneumophila—Recognized Public Health Risk Factor

Knežević

Rončević

Lušić

et al. 2022

IJERPH

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Legionellae are gram-negative bacteria most commonly found in freshwater ecosystems and purpose-built water systems. In humans, the bacterium causes Legionnaires’ disease (LD) or a Pontiac fever. In this study, the different waters (drinking water, pool water, cooling towers) in which Legionella pneumophila has been isolated were studied to assess the possible risk of bacterial spreading in the population. The influence of physical and chemical parameters, and interactions with Acanthamoeba castellanii on L. pneumophila, were analyzed by Heterotrophic Plate Count, the Colony-forming units (CFU) methods, transmission electron microscopy (TEM), and Sequence-Based Typing (SBT) analysis. During the study period (2013–2019), a total of 1932 water samples were analyzed, with the average annual rate of Legionella-positive water samples of 8.9%, showing an increasing trend. The largest proportion of Legionella-positive samples was found in cooling towers and rehabilitation centers (33.9% and 33.3%, respectively). Among the isolates, L. pneumophila SGs 2–14 was the most commonly identified strain (76%). The survival of Legionella was enhanced in the samples with higher pH values, while higher electrical conductivity, nitrate, and free residual chlorine concentration significantly reduced the survival of Legionella. Our results show that growth in amoeba does not affect the allelic profile, phenotype, and morphology of the bacterium but environmental L. pneumophila becomes more resistant to pasteurization treatment.

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Intracellular Behaviour of Legionella Non-pneumophila Strains within Three Amoeba Strains, Including Willaertia magna C2c Maky

Cited by 7 publications

References 59 publications

Pan-kinome of Legionella expanded by a bioinformatics survey

Pan-kinome of Legionella expanded by a bioinformatics survey

Microbiome predators in changing soils

Decreasing Pasteurization Treatment Efficiency against Amoeba-Grown Legionella pneumophila—Recognized Public Health Risk Factor

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