CAN VINASSE BE USED AS CARBON SOURCE FOR POLY(3-HYDROXYBUTYRATE) PRODUCTION BY Cupriavidus necator DSM 545?

Zanfonato, Kellen; Schmidt, Mélodi; Quines, Luci Kelin de Menezes; Gai, Claudia S.; Schmidell, Willibaldo; Aragão, Gláucia Maria Falcão de

doi:10.1590/0104-6632.20180353s20170265

Cited by 12 publications

(10 citation statements)

References 26 publications

(25 reference statements)

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“…Experimental studies reporting PHA production by C. necator from vinasses have been published recently by other authors. Zanfonato et al 46 carried out cultivations in a 5 L bioreactor using pure vinasses, vinasses with nitrogen, and vinasses with nitrogen and mineral salts. The best values reported for the total biomass (5.1 g/L) and PHB (26% of total biomass) were obtained in the Figure 4 Comparison of FTIR spectra between the polymer obtained by combined optimization (solid line) and the standard PHB (dashed-dotted line).…”

Section: Resultsmentioning

confidence: 99%

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Real‐time optimization and control for polyhydroxybutyrate fed‐batch production at pilot plant scale

Ochoa

Alcaraz

2020

J of Chemical Tech & Biotech

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BACKGROUND: Polyhydroxyalkanoate-type polymers (PHAs) are claimed as an alternative for replacing conventional plastics; but the economic feasibility of its production should still be assured in order to accomplish such a massive goal. In a previous work on fed-batch production of PHAs, it was shown that the process yield and the properties of the biopolymer obtained highly depend on the type of feeding strategy applied. Such results evidenced the need for implementing optimization-based control strategies to control the process in real-time, rather than implementing pre-defined feeding policies. RESULTS: In this study, real-time optimization for the fed-batch production of PHAs at pilot plant scale from a low-cost substrate (vinasses-molasses mixture) is presented. Two optimization-based control strategies were developed to calculate the optimal feed flow rate. One strategy considered only the process productivity as an objective, whereas the other incorporated not only productivity but also a profitability-oriented objective. The latter resulted in a productivity of 0.364 g/L h and a maximal polymer concentration of 62.03% cell dry weight, versus 0.043 g/L h and 6.25% attained by applying a traditional feedback control law. CONCLUSION: Implementation of closed-loop based-optimal control policies in PHA production has led to higher yield and polymer concentration, confirming that an improved operation of fed-batch bioprocesses is reached when the feeding policy is updated in real-time, according to an optimization goal. Despite implementation costs, applying this approach might be the best alternative for simultaneously facing uncertainties and achieving optimal operation at an industrial scale, especially when living microorganisms are used as factories.

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Section: Resultsmentioning

confidence: 99%

“…Experimental studies reporting PHA production by C. necator from vinasses have been published recently by other authors. Zanfonato et al 46 . carried out cultivations in a 5 L bioreactor using pure vinasses, vinasses with nitrogen, and vinasses with nitrogen and mineral salts.…”

Section: Resultsmentioning

confidence: 99%

Real‐time optimization and control for polyhydroxybutyrate fed‐batch production at pilot plant scale

Ochoa

Alcaraz

2020

J of Chemical Tech & Biotech

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“…In this sense, vinasse, a residue of the sugar cane alcohol industry, could be used as an economic substrate to produce PHAs at lower cost and some authors have reported the use of this by-product as carbon source for the production of PHAs [9] . Among others, Zanfonato et al [10] did fermentations with Cupriavidus necator and Pramanik et al [11] withHaloarcula marismortui using vinasse as carbon source to produce PHAs, obtaining a good productivity. Sugarcane vinasse is an industrial effluent with dark color, acid pH, high sulfur content and characteristic odor [12] .…”

Section: Introductionmentioning

confidence: 99%

“…Around 10 to 16 L of vinasse are obtained per liter of ethanol. Increasing demands fuel in recent years will increase ethanol production and, consequently, the generation of vinasse [10] . Within this context, the use of vinasse as raw material to obtain new high added-value products could be a response to reduce the environmental impact and get an economic profit.…”

Section: Introductionmentioning

confidence: 99%

Economic feasibility of exploitation of a bioethanol by-product to obtain high-value biopolymers

Trapé

Ramos

López

et al. 2022

Preprint

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By-products of bioethanol production, such as vinasse can be transformed by biotechnological processes into valuable compounds. In this work, the economic feasibility of producing poly(hydroxyalcanoate)s (PHAs) from vinasse and diverse microorganisms was studied employing a superstructure whose objective function is the maximization of the Net Present Value (NPV). The model was run employing data reported in the literature about PHAs productivities obtained by using different bacteria strains and vinasse as carbon source. Thus, comparison of different scenarios is shown from techno-economic and environmental aspects. Mathematical model estimated that PHA production cost was 2.27 - 3.02 USD and the investment resulted 9- 13 million dollars. The energy consumption was also calculated and it was 13.8 - 21 MJ/Kg PHA. The model returned NPV negative values in those scenarios with low accumulation and low yield of PHAs, indicating that benefits are not being obtained and, therefore, would not meet the expectations for an investment project. Positive NPV values were obtained, being the highest one 35.09 million dollars. Results evidenced that microorganism selection, concentration of the pretreated vinasse, and inorganic salts in the culture media would be the most relevant factors to take into account when designing a PHA production plant.

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“…In this sense, some authors have reported the use of vinasse as carbon source for PHB production. Zanfonato et al (2018) did fermentations with Cupriavidus necator and Pramanik et al (2012) with Haloarcula marismortui using vinasse as carbon source to produce PHB. In other cases, a combination of vinasse and sugarcane molasses was used by Cupriavidus necator as microorganism (Dalsasso et al 2019,Acosta-Cárdenas et al 2018).…”

Section: Introductionmentioning

confidence: 99%

Vinasse: From A Residue To A High Added Value Biopolymer

Trapé¹,

López²,

Villar³

2021

Preprint

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This work aimed to study the feasibility to use vinasse for polyhydroxybutyrate (PHB) production by Bacillus megaterium. To optimize the culture medium, a Box Behnken design was employed considering carbon (C), nitrogen (N), and phosphorus (Ph) concentrations as independent variables and PHB productivity as the response variable. The productivity decreased when C or N were increased, probably due to the presence of phenolic compounds and the limitation of N for the production of PHB by Bacillus sp. bacteria. An additional experimental design to optimize the C/N ratio and growing conditions (fermentation time and temperature) was carried out. Fermentation time had a statistically significant effect on PHB productivity reaching 10.6 mg/L h. On the other hand, the variability in physicochemical properties of vinasse samples led to significant differences in PHB productivity. Lower productivity values were obtained when vinasse had DBO high values. Therefore, biopolymers production from vinasse is a feasible alternative to valorize this bioethanol by-product.

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CAN VINASSE BE USED AS CARBON SOURCE FOR POLY(3-HYDROXYBUTYRATE) PRODUCTION BY Cupriavidus necator DSM 545?

Cited by 12 publications

References 26 publications

Real‐time optimization and control for polyhydroxybutyrate fed‐batch production at pilot plant scale

Real‐time optimization and control for polyhydroxybutyrate fed‐batch production at pilot plant scale

Economic feasibility of exploitation of a bioethanol by-product to obtain high-value biopolymers

Vinasse: From A Residue To A High Added Value Biopolymer

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