Can precancerous stem cells be risk markers for malignant transformation in the oral mucosa?

Wang, Shan; Liu, Ying; Yu, Shuyi; Bai, Jie; Hao, Chunbo

doi:10.1186/s11658-023-00441-0

Cited by 3 publications

(1 citation statement)

References 65 publications

(102 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Chromosomal instability (CIN) is an important trait of tumor cells and is characterized by abnormal numbers and structures. CIN is commonly found in a variety of solid tumors and malignant hematological diseases [ 5 , 6 ]. Studies have shown that specific marker chromosomes have become pivotal targets for the diagnosis, treatment and prognostic prediction of chronic myeloid leukemia and other tumors [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

MYCT1 inhibits hematopoiesis in diffuse large B-cell lymphoma by suppressing RUNX1 transcription

Liang,

Wei,

Yue

et al. 2024

Cell Mol Biol Lett

View full text Add to dashboard Cite

Background The abnormality of chromosomal karyotype is one factor causing poor prognosis of lymphoma. In the analysis of abnormal karyotype of lymphoma patients, three smallest overlap regions were found, in which MYCT1 was located. MYCT1 is the first tumor suppressor gene cloned by our research team, but its studies relating to the occurrence and development of lymphoma have not been reported. Methods R banding analyses were employed to screen the abnormality of chromosomal karyotype in clinical specimen and MYCT1 over-expression cell lines. FISH was to monitor MYCT1 copy number aberration. RT-PCR and Western blot were to detect the mRNA and protein levels of the MYCT1 and RUNX1 genes, respectively. The MYCT1 and RUNX1 protein levels in clinical specimen were evaluated by immunohistochemical DAB staining. The interaction between MYCT1 and MAX proteins was identified via Co-IP and IF. The binding of MAX on the promoter of the RUNX1 gene was detected by ChIP and Dual-luciferase reporter assay, respectively. Flow cytometry and CCK-8 assay were to explore the effects of MYCT1 and RUNX1 on the cell cycle and proliferation, respectively. Results MYCT1 was located in one of three smallest overlap regions of diffuse large B-cell lymphoma, it altered chromosomal instability of diffuse large B-cell lymphoma cells. MYCT1 negatively correlated with RUNX1 in lymphoma tissues of the patients. MAX directly promoted the RUNX1 gene transcription by binding to its promoter region. MYCT1 may represses RUNX1 transcription by binding MAX in diffuse large B-cell lymphoma cells. MYCT1 binding to MAX probably suppressed RUNX1 transcription, leading to the inhibition of proliferation and cell cycle of the diffuse large B-cell lymphoma cells. Conclusion This study finds that there is a MYCT1-MAX-RUNX1 signaling pathway in diffuse large B-cell lymphoma. And the study provides clues and basis for the in-depth studies of MYCT1 in the diagnosis, treatment and prognosis of lymphoma.

show abstract

Section: Introductionmentioning

confidence: 99%

MYCT1 inhibits hematopoiesis in diffuse large B-cell lymphoma by suppressing RUNX1 transcription

Liang,

Wei,

Yue

et al. 2024

Cell Mol Biol Lett

View full text Add to dashboard Cite

show abstract

Mesenchymal stem cells in the management of oral submucous fibrosis—An enigma to be solved

Kizhakkoottu,

Ramani,

Tilakaratne

2024

J Oral Pathology Medicine

View full text Add to dashboard Cite

Possible role of ALDH1 and CD44 in lip carcinogenesis

ORTIZ,

GOIS,

COSTA

et al. 2023

J. Appl. Oral Sci.

View full text Add to dashboard Cite

Possible role of ALDH1 and CD44 in lip carcinogenesisLip squamous cell carcinoma (LSCC) accounts for 12% of all head and neck cancers. It is caused by chronic exposure to ultraviolet light solar radiation and related to previous actinic cheilitis (AC). This study aimed to investigate the immunostaining of the putative cancer stem cells (CSC) markers ALDH1 and CD44 in AC (n=30) and LSCC (n=20). ALDH1 positivity was found to be statistically higher in LSCC than in AC lesions (p=0.0045), whilst CD44 expression was statistically higher in AC than in LSCC lesions (p=0.0155).ALDH1+ cells in AC lesions were associated with specific clinical features: a younger age (<60 years old), the female gender, white skin, not smoking or consuming alcohol, and a fast evolution, and not associated with the chronic exposure to UV radiation (p<0.0001). CD44 positivity was associated with patients who were male, feoderm, smoked, consumed alcohol, underwent occupational exposure to UV-radiation, and demonstrated lesions with log-time evolution (p<0.0001). ALDH1 + cells were associated with mild dysplasia using a system from the World Health Organization (WHO), and with a low risk of malignant transformation, according to the binary system (p<0.0001). CD44+ cells were also associated with moderated dysplasia, according to the WHO system. In LSCC, ALDH1 + cells were positively associated with patients who were older (≥ 60 years old), smokers, and with those who consumed alcohol (p<0.0001). CD44 + cells in LSCC were associated with older (≥ 60 years old) patients as well, but also with female patients, white skin, non-smokers, and individuals who did not consume alcohol (p<0.0001), all of whom showed distinct patterns in pre-and malignant lesions of both markers. Additionally, in LSCC, both ALDH1 and CD44 staining were associated with smaller tumor sizes (T1/T2; p<0.0001). In summary, although both ALDH1 and CD44 were associated with the presence of dysplasia in AC lesions, the present findings suggest that ALDH1 and CD44 may be activated by different etiopathogenic pathways, predominantly in distinct steps of oral carcinogenesis. CD44 would thus be more significantly related to the potentially malignant lesion, while ALDH1 would be closely linked to malignancy.

show abstract

Can precancerous stem cells be risk markers for malignant transformation in the oral mucosa?

Cited by 3 publications

References 65 publications

MYCT1 inhibits hematopoiesis in diffuse large B-cell lymphoma by suppressing RUNX1 transcription

MYCT1 inhibits hematopoiesis in diffuse large B-cell lymphoma by suppressing RUNX1 transcription

Mesenchymal stem cells in the management of oral submucous fibrosis—An enigma to be solved

Possible role of ALDH1 and CD44 in lip carcinogenesis

Contact Info

Product

Resources

About