Can Ocimum basilicum L. and Ocimum tenuiflorum L. in vitro culture be a potential source of secondary metabolites?

Bhuvaneshwari, K.; Gokulanathan, Ananda; Jayanthi, Malaiyandi; Govindasamy, V.; Milella, Luigi; Lee, Sungyoung; Yang, Deok Chun; Suja, G.

doi:10.1016/j.foodchem.2015.07.136

Cited by 27 publications

(16 citation statements)

References 28 publications

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“…The basil Ocimum basilicum L. (Lamiales: Lamiaceae) is an aromatic plant, cultivated in many countries due to its economic importance and great adaptability (Carović-Stanko et al, 2010). The Ocimum genus has aromatic compounds as methyl eugenol, linalool, and, specially, eugenol, which are the most important constituents of the basil essential oil (Bhuvaneshwari et al 2016). These compounds have nematicidal, pro-oxidants, anti-oxidants, dental anesthetics and disinfectant activity, as well as protect agents against nicotine toxicity in murine peritoneal macrophages (Kaya et al, 2008;Anamika et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Parasitoids diversity in organic Sweet Pepper (Capsicum annuum) associated with Basil (Ocimum basilicum) and Marigold (Tagetes erecta )

Souza

Tomazella

Santos³

et al. 2019

Braz. J. Biol.

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The sweet pepper (Capsicum annuum L.) is one of the most important crops in Brazilian farming. Many insect are related to this crop, compromising the quantity and quality of the fruit, representing a production problem. Vegetable diversification is one of the main elements that can be managed for suppressing undesirable insect populations in organic production, once that supports the presence of natural enemies. The basil Ocimum basilicum L. and the marigold Tagetes erecta L. are attractive and nutritious plants for parasitoids, being important candidates for diversified crops. This study evaluated the parasitoids attracted by the association of basil and marigold to organic sweet pepper crop. The experiment comprised three treatments: a) sweet pepper monoculture; b) sweet pepper and basil intercropping; c) sweet pepper and marigold intercropping. Hymenopteran parasitoids were collected over 14 weeks. 268 individuals from 12 families and 41 taxa were collected. Sweet pepper monoculture, sweet pepper-basil intercropping, and sweet pepper-marigold intercropping hosted 40, 98, and 130 individuals and richness of 24, 24, and 23, respectively. Furthermore, the insects of greater abundance in the basil and marigold were different to those collected in the monoculture. The number of parasitoids increased in the associations of sweet pepper with basil and marigold, providing advantages in the use of vegetable diversification for the organic pepper crops management.

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Section: Introductionmentioning

confidence: 99%

Parasitoids diversity in organic Sweet Pepper (Capsicum annuum) associated with Basil (Ocimum basilicum) and Marigold (Tagetes erecta )

Souza

Tomazella

Santos³

et al. 2019

Braz. J. Biol.

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“…In addition, through in vitro cultivation techniques, it is possible to optimize the production of secondary metabolites with ethnopharmacological importance in several species (Bhuvaneshwari et al, 2016;Venugopalan;Srivastava, 2015). In this context, considering that there is little information regarding the in vitro culture of G. americana, this study aimed to optimize the in vitro germination of G. americana seeds by identifying internal and external factors affecting the germination process.…”

Section: Introductionmentioning

confidence: 99%

Optimization of jenipapo in vitro seed germination process

et al. 2016

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The in vitro seed germination is an effective alternative for quickly obtaining explants with sanitary quality. However, jenipapo seeds present slow and uneven germination. Therefore, internal and external factors to seed which directly interfere in the process, they must be identified, in order to adapt better techniques to obtain seedlings. In this sense, this work aimed to optimize the in vitro germination of Genipa americana L. seeds by evaluating different factors (light quality, GA 3 treatment, pre-soaking in distilled water, growing media and stratification in the dark). It was found that the seed germination of G. americana was indifferent to light, however, the best results were obtained under conditions of continuous darkness; There was no effect of the application of exogenous GA 3 ; The pre-soaking in distilled water for 48 h contributes to obtaining better germination rates; And the reduction in MS medium salts, and laminating the pretreatment in the dark maximizes the germination potential of seeds.Therefore, the optimal conditions for in vitro germination of G. americana L. seeds requires pre-soaking in distilled water for 48 hours and inoculation into culture media consisting of ½ MS + 15 g L -1 sucrose, with stratification in the dark for 16 days, followed by the transfer to growth chambers with lighting provided by white fluorescent lamps.Index terms: Genipa americana L.; in vitro culture; ligth quality; Rubiaceae; stratification in the dark. RESUMOA germinação in vitro de sementes é uma alternativa eficaz para obtenção de explantes com rapidez e qualidade sanitária. No entanto, sementes de jenipapeiro apresentam germinação lenta e desuniforme. Para tanto, fatores internos e externos às sementes que interferem diretamente no processo, devem ser identificados, visando à adaptação de melhores técnicas para obtenção de mudas. Neste sentido, objetivou-se otimizar a germinação in vitro de sementes de Genipa americana L. por meio da avaliação de diferentes fatores (qualidade de luz, tratamento com GA 3 , pré-embebição em água destilada, diferentes composições no meio de cultivo e estratificação no escuro). Verificou-se que a germinação das sementes foi indiferente a luz, no entanto, os melhores resultadados foram obtidos sob condições de escuro contínuo; Não houve efeito da aplicação de GA 3 exógeno; A pré-embebição em água destilada durante 48h contribui para obtenção de melhores taxas de germinação; E a redução de sais do meio MS, bem como o pré-tratamento de estratificação no escuro maximizam o potencial germinativo das sementes. Portanto, as condições ótimas para germinação in vitro de sementes de G. americana L. requer a pré-embebição em água destilada por 48 horas e a inoculação em meio de cultivo constituído de ½ MS + 15 g L-1 de sacarose, com estratificação no escuro por 16 dias, seguida pela transferência a salas de crescimento com iluminação fornecida por lâmpadas fluorescentes branca.Termos para indexação: Genipa americana L.; cultivo in vitro; qualidade de luz; Rubiaceae; estratif...

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“…A standard curve was generated from integrated peak area and concentration of the same standard expressed as % recovery of the sample. The equations cover the range of concentration used in sample determination, having acceptable correlation coefficient (r>0.999) [15].…”

Section: Stigmasterol Content Determinationmentioning

confidence: 99%

Stigmasterol Content of Artemisia Annua L. And the Phytosterol Profile

Tofiana¹,

Iwo²,

Kartasasmita³

et al. 2016

Asian J Pharm Clin Res

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ABSTRACT Objective: To find the stigmasterol source of Artemisia annua L. and to study the profile of phytosterol types in A. annua hairy root cultures. Methods: Stigmasterol content determination was done based on stigmasterol content in the methylene chloride extract of A. annua using high performance liquid chromatography in wild-type plant, plant tissue cultures, and hairy root cultures. The optimal resolution was achieved by suitability system, and stigmasterol calibration curve was generated with stigmasterol concentrations series. Phytosterol types in A. annua hairy root cultures were performed by gas chromatography-mass spectrophotometry. Results: Highest stigmasterol content in A. annua is shown by hairy root cultures of A. annua in the 5 week harvesting time, which is 1.5 g/100 g (w/w) extract. The phytosterol profile in A. annua hairy root cultures encompassed stigmasterol 74.6%, β-sitosterol 13.5%, and campesterol 11.9%. th Conclusion: A. annua hairy root cultures in 5-week harvesting time prove its potential as stigmasterol source alternative. The highest phytosterol type in A. annua hairy root cultures is stigmasterol. This is the first report stigmasterol content and its phytosterol profile of A. annua hairy root cultures extract. Keywords: Artemisia annua L., Wild-type plant, Plant tissue cultures, Hairy root cultures, Stigmasterol.

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Can Ocimum basilicum L. and Ocimum tenuiflorum L. in vitro culture be a potential source of secondary metabolites?

Cited by 27 publications

References 28 publications

Parasitoids diversity in organic Sweet Pepper (Capsicum annuum) associated with Basil (Ocimum basilicum) and Marigold (Tagetes erecta )

Parasitoids diversity in organic Sweet Pepper (Capsicum annuum) associated with Basil (Ocimum basilicum) and Marigold (Tagetes erecta )

Optimization of jenipapo in vitro seed germination process

Stigmasterol Content of Artemisia Annua L. And the Phytosterol Profile

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