<p>ABSTRACT<br />Objective: To find the stigmasterol source of Artemisia annua L. and to study the profile of phytosterol types in A. annua hairy root cultures.<br />Methods: Stigmasterol content determination was done based on stigmasterol content in the methylene chloride extract of A. annua using high<br />performance liquid chromatography in wild-type plant, plant tissue cultures, and hairy root cultures. The optimal resolution was achieved by<br />suitability system, and stigmasterol calibration curve was generated with stigmasterol concentrations series. Phytosterol types in A. annua hairy root<br />cultures were performed by gas chromatography-mass spectrophotometry.<br />Results: Highest stigmasterol content in A. annua is shown by hairy root cultures of A. annua in the 5<br /> week harvesting time, which is 1.5 g/100 g (w/w)<br />extract. The phytosterol profile in A. annua hairy root cultures encompassed stigmasterol 74.6%, β-sitosterol 13.5%, and campesterol 11.9%.<br />th<br />Conclusion: A. annua hairy root cultures in 5-week harvesting time prove its potential as stigmasterol source alternative. The highest phytosterol type<br />in A. annua hairy root cultures is stigmasterol. This is the first report stigmasterol content and its phytosterol profile of A. annua hairy root cultures<br />extract.<br />Keywords: Artemisia annua L., Wild-type plant, Plant tissue cultures, Hairy root cultures, Stigmasterol.</p>
Pandemi Covid-19 yang melanda hampir di seluruh dunia di awal tahun 2020, telah banyak mengubah pandangan masyarakat terhadap pemanfaatan bahan alam sebagai pengobatan.Salah satunya adalah pemanfaatan minyak kayu putih yang di klaim dapat mengobati Covid19, padahal minyak kayu putih selama ini diketahui hanya sediaan topikal yang membantumelegakan hidung dan tenggorokan. Pemanfaatan bahan alam baik berasal dari tumbuhan, hewan, mineral dan/atau campurannya tidak dapat serta merta diterima sebagai bahan obat (obat bahan alam) dan berfungsi sebagai pengobatan manakalah bahan alam tersebut belum memiliki evidence base yang memadai, baik dari sisi keamanan dan manfaat. Minyak kayu putih dapat diklaim mengobati Covid-19 setelah hasil uji pra klinik dan uji klinik membuktikan hal tersebut. Dan juga bukan misal karena resep dokter, bahan tersebut menjadi dapat digunakan sebagai pengobatan. Tujuan penulisan ini adalah memberikan alternatif tentang kapan resep dokter untuk obat bahan alam diberikan melalui pendekatan logic frame,disandingkan dengan pemberian resep dokter pada obat modern atau obat kimia.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
customersupport@researchsolutions.com
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.