Can crude alkaloids extract of Rhazya stricta induce apoptosis in pancreatic cancer: In vitro study?

Shaer, Nehad A.

doi:10.1016/j.pathophys.2018.09.001

Cited by 7 publications

(6 citation statements)

References 20 publications

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“…The study of Shaer et al showed that the crude alkaloids extract of Rhazya stricta significantly induced apoptotic death in PC cells. They observed a significant decrease in cell viability in a dose-dependent manner [197].…”

Section: Rhazya Strictamentioning

confidence: 98%

Herbals and Plants in the Treatment of Pancreatic Cancer: A Systematic Review of Experimental and Clinical Studies

et al. 2022

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Background: Pancreatic cancer represents the most lethal malignancy among all digestive cancers. Despite the therapeutic advances achieved during recent years, the prognosis of this neoplasm remains disappointing. An enormous amount of experimental (mainly) and clinical research has recently emerged referring to the effectiveness of various plants administered either alone or in combination with chemotherapeutic agents. Apart from Asian countries, the use of these plants and herbals in the treatment of digestive cancer is also increasing in a number of Western countries as well. The aim of this study is to review the available literature regarding the efficacy of plants and herbals in pancreatic cancer. Methods: The authors have reviewed all the experimental and clinical studies published in Medline and Embase, up to June 2021. Results: More than 100 plants and herbals were thoroughly investigated. Favorable effects concerning the inhibition of cancer cell lines in the experimental studies and a favorable clinical outcome after combining various plants with established chemotherapeutic agents were observed. These herbals and plants exerted their activity against pancreatic cancer via a number of mechanisms. The number and severity of side-effects are generally of a mild degree. Conclusion: A quite high number of clinical and experimental studies confirmed the beneficial effect of many plants and herbals in pancreatic cancer. More large, double-blind clinical studies assessing these natural products, either alone or in combination with chemotherapeutic agents should be conducted.

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Section: Rhazya Strictamentioning

confidence: 98%

Herbals and Plants in the Treatment of Pancreatic Cancer: A Systematic Review of Experimental and Clinical Studies

et al. 2022

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“…E, which was eluted by 50% EtOAc in CHCl 3 , was purified by PTLC using CHCl 3 : MeOH (9:1, v/v; 1, 0.8 mg, R f value = 0.55). Another 1 kg of the aerial parts of R. stricta was extracted directly with acidified water and then was processed as previously reported [15]. The crude basic residue (15.0 g) was fractionated on an Al 2 O 3 column, employing the gradient technique starting from CHCl 3 , to EtOAc, and then to MeOH.…”

Section: Extraction and Isolationmentioning

confidence: 99%

“…It includes two species, Rhazya stricta (R. stricta) and Rhazya orientalis. In the Arabian Peninsula, R. stricta is used in folk medicine for the treatment of several diseases [15][16][17]. Several MIAs as leepacine, aspidospermiose, aspidospermidose, strictanol, strictanine, and strictibine are terpenoidal were reported from R. stricta.…”

Section: Introductionmentioning

confidence: 99%

Monoterpene Indole Alkaloids from the Aerial Parts of Rhazya stricta Induce Cytotoxicity and Apoptosis in Human Adenocarcinoma Cells

et al. 2022

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Chromatographic investigation of the aerial parts of the Rhazya stricta (Apocynaceae) resulted in the isolation of two new monoterpene indole alkaloids, 6-nor-antirhine-N1-methyl (1) and razyamide (2), along with six known compounds, eburenine (3), epi-rhazyaminine (4), rhazizine (5), 20-epi-sitsirikine (6), antirhine (7), and 16-epi-stemmadenine-N-oxide (8). The chemical structures were established by various spectroscopic experiments. Compounds 1–8 exhibited cytotoxic effects against three cancer cells with IC50 values ranging between 5.1 ± 0.10 and 93.2 ± 9.73 µM against MCF-7; 5.1 ± 0.28 and 290.2 ± 7.50 µM against HepG2, and 3.1 ± 0.17 and 55.7 ± 4.29 µM against HeLa cells. Compound 2 showed the most potent cytotoxic effect against all cancer cell lines (MCF-7, HepG2 and HeLa with IC50 values = 5.1 ± 0.10, 5.1 ± 0.28, and 3.1 ± 0.17 µM, respectively). Furthermore, compound 2 revealed a significant increase in the apoptotic cell population of MCF-7, HepG2, and HeLa cells, with 31.4 ± 0.2%, 29.2 ± 0.5%, and 34.9 ± 0.6%, respectively. Compound 2 decreased the percentage of the phagocytic pathway on HepG2 cells by 15.0 ± 0.1%. These findings can explain the antiproliferative effect of compound 2.

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“…This effect may be a result of the compound's hepatoprotective properties, antiproliferative activity, and antiangiogenic potential [60]. The crude alkaloid extract of R. stricta significantly induced apoptosis in pancreatic cancer cells with IC50 (78.77 and 41.4 µg/mL) on PANC-1 and AsPC-1 cell lines [61]. The ethanol extract of R. stricta leaves suppressed colony formation development in HepG2 cells and significantly restricted cell cycle in the G2/M phase 12 and 48 h following administration, as well as substantial limitation at the G1/S phase after 24 h. This finding supports the use of R. stricta as a novel anticancer agent in the treatment of hepatocellular carcinoma [62].…”

Section: Anticancer Activitymentioning

confidence: 99%

A Review of Rhazya stricta Decne Phytochemistry, Bioactivities, Pharmacological Activities, Toxicity, and Folkloric Medicinal Uses

Albeshri¹,

Baeshen²,

Bouback

et al. 2021

Plants

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The local medicinal plant Rhazya stricta Decne is reviewed for its folkloric medicinal, phytochemical, pharmacological, biological, and toxicological features. R. stricta has been used widely in different cultures for various medical disorders. The phytochemical studies performed on the R. stricta extract revealed many alkaloidal and fatty acid compounds. Moreover, several flavonoid and terpenoid compounds were also detected. Pharmacological activates of R. stricta extracts are approved to possess antimicrobial, antioxidant, anticancer, antidiabetic, and antihypertensive activities. Additionally, R. stricta extract was found to hold biological activates such as larvicidal and phytoremediation activates R. stricta extract was found to be toxic, genotoxic, and mutagenic. R. stricta contains novel phytochemical compounds that have not been investigated pharmacologically. Further research is needed through in vitro and in vivo experiments to pave the road for these compounds for medical, veterinary, and ecological uses.

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Can crude alkaloids extract of Rhazya stricta induce apoptosis in pancreatic cancer: In vitro study?

Cited by 7 publications

References 20 publications

Herbals and Plants in the Treatment of Pancreatic Cancer: A Systematic Review of Experimental and Clinical Studies

Herbals and Plants in the Treatment of Pancreatic Cancer: A Systematic Review of Experimental and Clinical Studies

Monoterpene Indole Alkaloids from the Aerial Parts of Rhazya stricta Induce Cytotoxicity and Apoptosis in Human Adenocarcinoma Cells

A Review of Rhazya stricta Decne Phytochemistry, Bioactivities, Pharmacological Activities, Toxicity, and Folkloric Medicinal Uses

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