Can CRISPR/Cas Technology Be a Felicitous Stratagem Against the COVID-19 Fiasco? Prospects and Hitches

Konwarh, Rocktotpal

doi:10.3389/fmolb.2020.557377

Cited by 16 publications

(9 citation statements)

References 54 publications

(58 reference statements)

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“…SARS-CoV-2 is a Coronaviridae virus with an enveloped RNA genome that causes moderate to extreme symptoms in people of all ages [73,74]. A novel approach to reducing SARS-CoV-2 replication to fight viral attack appears to be using the CRISPR/ Cas system by targeting the positive-sense genome and viral mRNAs for endogenous closing viral genome templates and switching off their gene expression [75][76][77].…”

Section: Viral Infections and Crispr/casmentioning

confidence: 99%

Exploring nano-enabled CRISPR-Cas-powered strategies for efficient diagnostics and treatment of infectious diseases

et al. 2022

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Biomedical researchers have subsequently been inspired the development of new approaches for precisely changing an organism's genomic DNA in order to investigate customized diagnostics and therapeutics utilizing genetic engineering techniques. Clustered Regulatory Interspaced Short Palindromic Repeats (CRISPR) is one such technique that has emerged as a safe, targeted, and effective pharmaceutical treatment against a wide range of disease-causing organisms, including bacteria, fungi, parasites, and viruses, as well as genetic abnormalities. The recent discovery of very flexible engineered nucleic acid binding proteins has changed the scientific area of genome editing in a revolutionary way. Since current genetic engineering technique relies on viral vectors, issues about immunogenicity, insertional oncogenesis, retention, and targeted delivery remain unanswered. The use of nanotechnology has the potential to improve the safety and efficacy of CRISPR/ Cas9 component distribution by employing tailored polymeric nanoparticles. The combination of two (CRISPR/Cas9 and nanotechnology) offers the potential to open new therapeutic paths. Considering the benefits, demand, and constraints, the goal of this research is to acquire more about the biology of CRISPR technology, as well as aspects of selective and effective diagnostics and therapies for infectious illnesses and other metabolic disorders. This review advocated combining nanomedicine (nanomedicine) with a CRISPR/Cas enabled sensing system to perform early-stage diagnostics and selective therapy of specific infectious disorders. Such a Nano-CRISPR-powered nanomedicine and sensing system would allow for successful infectious illness control, even on a personal level. This comprehensive study also discusses the current obstacles and potential of the predicted technology.

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Section: Viral Infections and Crispr/casmentioning

confidence: 99%

Exploring nano-enabled CRISPR-Cas-powered strategies for efficient diagnostics and treatment of infectious diseases

et al. 2022

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“…The CRISPR–Cas system has been successfully employed for COVID-19 detection. To date, there are a few reported methods derived from CRISPR that can detect SARS-CoV-2, the virus that causes COVID-19. ,,− It has been comprehensively reviewed elsewhere. − As an example, a SARS-CoV-2 DNA endonuclease-targeted CRISPR trans reporter (DETECTR) technique was developed to diagnose COVID-19 by analyzing the extracts from the nasopharyngeal or oropharyngeal swabs . The DETECTR assay combined loop-mediated amplification (RT–LAMP) and reverse transcription to detect the genome of SARS-CoV-2, followed by the detection of its predefined N (nucleoprotein) and E (envelope) genes.…”

Section: Applications Of Mds-derived Biosensingmentioning

confidence: 99%

New Insights for Biosensing: Lessons from Microbial Defense Systems

Wan

Zong

et al. 2022

Chem. Rev.

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Microorganisms have gained defense systems during the lengthy process of evolution over millions of years. Such defense systems can protect them from being attacked by invading species (e.g., CRISPR−Cas for establishing adaptive immune systems and nanopore-forming toxins as virulence factors) or enable them to adapt to different conditions (e.g., gas vesicles for achieving buoyancy control). These microorganism defense systems (MDS) have inspired the development of biosensors that have received much attention in a wide range of fields including life science research, food safety, and medical diagnosis. This Review comprehensively analyzes biosensing platforms originating from MDS for sensing and imaging biological analytes. We first describe a basic overview of MDS and MDS-inspired biosensing platforms (e.g., CRISPR−Cas systems, nanopore-forming proteins, and gas vesicles), followed by a critical discussion of their functions and properties.We then discuss several transduction mechanisms (optical, acoustic, magnetic, and electrical) involved in MDS-inspired biosensing. We further detail the applications of the MDS-inspired biosensors to detect a variety of analytes (nucleic acids, peptides, proteins, pathogens, cells, small molecules, and metal ions). In the end, we propose the key challenges and future perspectives in seeking new and improved MDS tools that can potentially lead to breakthrough discoveries in developing a new generation of biosensors with a combination of low cost; high sensitivity, accuracy, and precision; and fast detection. Overall, this Review gives a historical review of MDS, elucidates the principles of emulating MDS to develop biosensors, and analyzes the recent advancements, current challenges, and future trends in this field. It provides a unique critical analysis of emulating MDS to develop robust biosensors and discusses the design of such biosensors using elements found in MDS, showing that emulating MDS is a promising approach to conceptually advancing the design of biosensors.

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“…Broughton and colleagues reported the detection by CRISPR, a powerful gene-editing tool, of viral RNA extracts from nasopharyngeal swabs, together with a lateral flow test that uses the AuNP label. [106][107][108] F I G U R E 1 0 LNP-encapsulation mRNA encoding S Protein (created with BioRender.com) F I G U R E 1 1 Schematic representation of an immunochromatographic blood or serum antibodies test, using AuNP lable for direct visualization (adapted from the advanced healthcare materials 105 ) This method has a high sensitivity for SARS-CoV-2 identification without cross-reactivity for related coronavirus strains. Similarly, a portable built-in microdevice that combines RT-PCR and ICT was developed for the H1N1 colorimetric detection was reported a few years ago.…”

Section: Nanomaterials For the Detection Of Sars-cov-2mentioning

confidence: 99%

Nano‐therapeutic strategies to target coronavirus

Rauf

Tasleem

Bhise

et al. 2021

VIEW

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The coronaviruses have caused severe acute respiratory syndrome (SARS), the Middle East respiratory syndrome (MERS), and the more recent coronavirus pneumonia . The global COVID-19 pandemic requires urgent action to develop anti-virals, new therapeutics, and vaccines. In this review, we discuss potential therapeutics including human recombinant ACE2 soluble, inflammatory cytokine inhibitors, and direct anti-viral agents such as remdesivir and favipiravir, to limit their fatality. We also discuss the structure of the SARS-CoV-2, which is crucial to the timely development of therapeutics, and previous attempts to generate vaccines against SARS-CoV and MERS-CoV. Finally, we provide an overview of the role of nanotechnology in the development of therapeutics as well as in the diagnosis of the infection. This information is key for computational modeling and nanomedicine-based new therapeutics by counteracting the variable proteins in the virus. Further, we also try to effectively share the latest information about many different aspects of COVID-19 vaccine developments and possible management to further scientific endeavors.

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Can CRISPR/Cas Technology Be a Felicitous Stratagem Against the COVID-19 Fiasco? Prospects and Hitches

Cited by 16 publications

References 54 publications

Exploring nano-enabled CRISPR-Cas-powered strategies for efficient diagnostics and treatment of infectious diseases

Exploring nano-enabled CRISPR-Cas-powered strategies for efficient diagnostics and treatment of infectious diseases

New Insights for Biosensing: Lessons from Microbial Defense Systems

Nano‐therapeutic strategies to target coronavirus

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