Can autologous platelet rich plasma expand endometrial thickness and improve pregnancy rate during frozen-thawed embryo transfer cycle? A randomized clinical trial

Autologous Platelet-Rich Plasma Infusion to Improve Pregnancy Outcome in Suboptimal Endometrium: A Review

“…A randomized control trial was performed by Eftekhar and others [22] in which 83 women participated; 40 were allocated to the study group and received PRP infusion, while 43 were placed in the control group and did not receive PRP. Prior to PRP infusion on the 13th day of cycle, there were no significant differences in endometrial lining as measured between the two groups; however, after PRP infusion, significant differences were noted (8.80 vs. 8.04 mm).…”

“…Eftekhar et al: In this study, the case group was treated with PRP and increased HRT, and the control group was just treated with increased HRT. For all women, E 2 V was started at 6 mg/d, then increased to 10 mg/d [22]. PRP infusion occurred on the 13th day of cycle and endometrial lining was measured transvaginally.…”

“…Tandulwadkar et al used 10 ml of autologous blood into an unspecified amount of ACD-A; which was centrifuged sequentially utilizing first a soft spin for 15 minutes, followed by a hard spin for 6 minutes, again yielding between 0.5 and 0.8 ml of PRP [21]. Eftekhar and others used an alternative approach, collecting PRP on the 13th day of HRT by drawing 8.5 ml of peripheral venous blood into a syringe containing 1.5 ml of ACD-A that was then centrifuged for 10 minutes; following first centrifugation, the buffy coat and plasma layer were then removed and centrifuged again, yielding 1.5 ml of PRP [22]. Meanwhile, Hounyoung et al collected 18 ml of venous blood in a 30 ml syringe prepared with 2 CC of ACD-A and then centrifuged twice to obtain 0.7-1.0 ml of PRP [23].…”

“…Chang et al performed a secondary study as well, involving a larger cohort of patients compared against a control group, and performed PRP collection as previously reported [18,25]. All studies transfused the PRP into the endometrium using an IUI catheter, and then repeated intravaginal ultrasound 48 hours later to measure endometrial growth; patients who did not reach the desired lining thickness (>7 mm deemed adequate in all studies) were then treated with a second round of PRP infusion [18][19][20][21][22][23][24][25].…”

“…Of 346 patients, 313 underwent embryo transfer at either cleavage or blastocyst stage. The remaining 33 patients dropped out of the studies prior to embryo transfer due to persistently poor endometrial development [18][19][20][21][22][23][24][25].…”