Can argyrophilic nucleolar organizing region-associated protein amount be used for the detection of cardiac damage?

Abstract

<jats:sec><jats:title>Introduction:</jats:title><jats:p> Carbon monoxide (CO) is a colorless, tasteless, odorless, nonirritant gas and CO poisoning affects all organ systems. </jats:p></jats:sec><jats:sec><jats:title>Aim:</jats:title><jats:p> We aimed to detect any possible effects of CO exposure on the argyrophilic nucleolar organizing region (AgNOR)-associated protein synthesis of heart cells and whether there is any relationship between AgNOR protein amount and both carboxyhemoglobin (COHb) level and histopathological evaluation methods used for the detection of damage in heart tissue after CO exposure. </jats:p></jats:sec><jats:sec><jats:title>Materials and Methods:</jats:title><jats:p> The rats were divided into four groups (control, 1000, 3000, and 5000 ppm), each containing six rats. After CO intoxication, COHb levels were measured and the animals were killed on the 7th day. AgNOR staining was performed in the heart tissue. One hundred nuclei per rat were evaluated, and total AgNOR area/nuclear area and mean AgNOR number were analyzed for each nucleus. The CO exposure groups had significantly higher AgNOR values than the control group ( p &lt; 0.0001). According to cardiomyopathy (CMY) scoring methods, the differences between groups 3 and 4 and groups 1 and 2 were significant ( p &lt; 0.05). A significant positive correlation between AgNOR values and both CMY and COHb levels were detected. </jats:p></jats:sec><jats:sec><jats:title>Conclusion:</jats:title><jats:p> The detection of AgNOR protein amount may give information about the CMY levels and be used to detect the CO intoxication levels instead of COHb in later periods. </jats:p></jats:sec>

Investigation of argyrophilic nucleolar organizing region

“…NORs are surrounded by a great number of regulatory proteins in interphase and they are functional subunits of the nucleolus (23). We reported that the AgNOR protein amount increased depending on the increase of CO exposure (also it caused hypoxic condition) in the lung and heart cells in our previous studies (16)(17)(18). In our current study, the TAA/NA ratio and total AgNOR number per total nuclear number increased depending on the I/R injury in the tubular cells of kidney, too.…”

“…Bacause of the silver affi nity, those proteins are named argyrophilic NOR (AgNOR)-associated proteins and silver staining method is the most reliable to show nucleoli in interphase nuclei (6). A lot of studies have been carried out about the importance of AgNOR proteins such as benign and malignant thyroid tissue (7)(8)(9)(10)(11), hair root cells of humans with alopecia or healthy individuals (12,13), buccal epithelial cells of Down syndrome infants and healthy persons (14,15), the effects of CO exposure on the NOR protein synthesis of hearth and lung cells (16)(17)(18) and so on. However, to our knowledge, there are no researches in the literature about the association between AgNOR proteins and renal ischemia/reperfusion (IR) injury.…”

Karbon Monoksit Zehirlenmesi: Klinik Bulgular, Sonuçlar, İzlem, Zehirlenme Tanısı ve Tedavisi

“… Arterial blood gas analysis-metabolic acidosis (which may occur secondary to lactic acidosis that is a result of ischemia) can be detected. (21).…”

The investigation of the antitumoral effect of Cornus mas L in mice with ehrlich solid tumor

“…These regions can be stained with silver, when active. Depending on the silver, these proteins are called argyrophilic NOR (AgNOR) related proteins, and the silver staining method is the most suitable to indicate nucleoli in the interphase nuclei (16,17). There are several studies on the importance of interphase AgNOR quantifi cation in tumor pathology for prognostic and diagnostic defi nitions of different types of cancer (18).…”