Investigation of argyrophilic nucleolar organizing region

Abstract

BACKGROUND: Ischemia/reperfusion (I/R) injury is a complex event frequently observed in vascular surgery and can cause functional and structural cell damage. Nucleolar-organizing regions (NORs) are sites of the ribosomal genes located on chromosomes and can be stained with silver when they are active. Thus these proteins are named as argyrophilic-NOR (AgNOR)-associated proteins. We aimed to investigate any possible effects of renal I/R injury on the NOR protein synthesis and association between the AgNOR proteins amount and histopathological injuring score. METHODS: Nine female wistar-albino rats with weight of 200-250g were included into the study. The animals were randomly divided in two groups, a Control Group and an I/R Group. In I/R group, rats were subjected to 45 minutes of renal pedicle occlusion followed by 24 hours of reperfusion. In the control group no drug injections or ischemia reperfusion were performed in animals. Then histopathological injury score, mean AgNOR number and total AgNOR area/nuclear area (TAA/NA) were detected for each rat. RESULTS: The differences between control and I/R groups were signifi cant for histopathological injury scores (p = 0.016). Also the differences between control group and I/R group were signifi cant for mean AgNOR number (p = 0.000) and TAA/NA ratio (p = 0.000). Additionally, there was a positive correlation between TAA/NA ratio and histopathological injury score (r = 0.728; p = 0.026) and between mean AgNOR number and histopathological injury score (r = 0.670; p = 0.048). CONCLUSION: The detection of AgNOR proteins amount may be used as an indicator to obtain information about the cellular behaviour (self-protective mechanism of tubular epithelial cells) against I/R injury and cellular damage levels (Tab. 2, Fig. 4, Ref. 24). Text in PDF www.elis.sk.

The relationship of argyrophilic proteins of the nuclear‐organized regions and atopic dermatitis in children

“…Imamoglu et al [13] reported that the number of AgNOR was significantly increased in children with Down syndrome. Nisari et al [14] reported that the number of AgNOR proteins could be used as an indicator of ischaemia/reperfusion injury. Rao et al [15] found that the number of AgNORs increased significantly in lichen planus and leukoplakia cases.…”

“…[8][9][10] The relationship between AgNOR and various cancer types and some disorders such as leukoplakia, lichen planus, oral submucosal fibrous, prostatic hyperplasia, neuronal damage and Down syndrome was investigated. [12][13][14][15] However, there have been no reports investigating the relationship between AgNOR and atopic dermatitis. The aim of this study was to investigate the relationship between atopic dermatitis and AgNOR in paediatric patients.…”

The role of argyrophilic nucleolar organizing region-associated proteins in clinical exacerbation of chronic obstructive pulmonary disease

“…10 The simplicity and cost-effectiveness of the present AgNOR technique make this a valuable diagnostic method. 14,15 In addition to the detection of the number, size and shape of AgNORs, the measurement of the total AgNOR area and the total nuclear area used for the calculation of the TAA/TNA ratio is also important. 14 Calculation of the TAA/TNA ratio reflects the protein synthesis capacity of the cells, which provides information about the metabolic and proliferative activity of each cell.…”

“…14 Calculation of the TAA/TNA ratio reflects the protein synthesis capacity of the cells, which provides information about the metabolic and proliferative activity of each cell. 14,15…”

May argyrophilic nucleolar organizing region-associated protein synthesis be used for selecting the most reliable dose of drugs such as rhamnetin in cancer treatments?

“…Thus, those proteins are referred to as argyrophilic NOR-associated proteins (AgNORs), while the silver-staining method is the most reliable to show nucleoli during the interphase (24). Numerous studies have been carried out on the importance of the interphase quantity of AgNOR proteins in hair root cells of humans (25,26), buccal epithelial and blood cells of Down syndrome infants and healthy persons (27)(28)(29), possible effects of carbonmonoxide (CO) exposure on the AgNOR protein synthesis of the cells of the heart, lung and femoral muscle (30)(31)(32)(33) and ischemia/reperfusion injury (34). However, to our knowledge, there is no study about the relation between AgNOR proteins and the effects of rhamnetin exposure on EST in literature.…”