Campomanesia lineatifolia Ruiz &amp; Pavón (Myrtaceae): Isolation of major and minor compounds of phenolic-rich extract by high-speed countercurrent chromatography and anti-inflammatory evaluation

Neves, Nívea Cristina Vieira; Mello, Morgana Pinheiro de; Zaidan, Isabella; Sousa, Lirlândia P.; Braga, Alysson Vinícius; Machado, Renes R.; Kukuła-Koch, Wirginia; Boylan, Fábio; Caliari, Marcelo Vidigal; Castilho, Rachel Oliveira

doi:10.1016/j.jep.2023.116417

Cited by 4 publications

(1 citation statement)

References 72 publications

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“…High-speed countercurrent chromatography (HSCCC) has been successfully employed for separating various plant chemicals, including phenolic-rich extracts, flavonoids, and polar polyphenols [9][10][11][12]. In a recent advancement, we have developed an environmentally friendly method for isolating antibiotics using HSCCC with the reused stationary phase [13].…”

Section: Introductionmentioning

confidence: 99%

Isolation of Momilactones A and B from Rice Husk Using High-Speed Countercurrent Chromatography

NAKAMORI,

NUNOME,

TAKAYAMA

et al. 2024

Chromatography

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Momilactones, allelochemicals from rice plants, exhibit diverse effects, including functioning as crop-friendly pesticides, and possessing antifungal and anticancer properties. The present study aimed to optimize the isolation of momilactones A (MA) and B (MB) using high-speed countercurrent chromatography (HSCCC), prioritizing the minimization of organic solvent waste and utilizing discarded rice husk. By employing techniques such as solvent reuse and HSCCC, we successfully isolated the highly purified fractions of MA and MB (>95% purity, as verified by HPLC) from rice husk. The optimized two-phase solvent system (n-hexane/ethyl acetate/methanol/water, 7/3/5/5, v/v/v/v) was specifically chosen for its efficiency in HSCCC, applied to extracts from 2 kg of rice husk. The separation was performed using the lower mobile phase of the two-phase solvent system at a flow rate of 1.0 mL/min after rotating the multilayer coiled column at the revolution speed of 1000 rpm. The retention of the stationary phase was observed to be 77%. The purity of isolated MA (3.0 mg) and MB (2.0 mg) was determined to be 99.0% and 98.2% via LC-UV chromatogram, respectively. Significantly, the organic solvents used in the extraction and HSCCC processes were redistilled and recycled, leading to an over 80% reduction in solvent consumption. This study demonstrates the feasibility of a sustainable, economical, and effective chromatographic method for isolating allelochemicals from waste plant materials.

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Section: Introductionmentioning

confidence: 99%

Isolation of Momilactones A and B from Rice Husk Using High-Speed Countercurrent Chromatography

NAKAMORI,

NUNOME,

TAKAYAMA

et al. 2024

Chromatography

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Exploring anti‐inflammatory and antioxidant‐related quality markers of Artemisia absinthium L. based on spectrum–effect relationship

Wubuli,

Abdulla,

Zhao

et al. 2024

Phytochemical Analysis

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IntroductionArtemisia absinthium L. is a well‐known medicinal, aromatic, and edible plant with important medicinal and economic properties and a long history of use in treating liver inflammation and other diseases; however, there has been insufficient progress in quality control.ObjectiveThis study aimed to investigate the quality markers for the anti‐inflammatory and antioxidant activities of A. absinthium based on spectrum–effect relationship analysis.Materials and methodsEighteen batches of A. absinthium from different origins were used. Chemical fingerprints were obtained by ultra‐performance liquid chromatography (UPLC). The chemical compositions were identified by quadrupole‐Orbitrap high‐resolution mass spectrometry. Anti‐inflammatory activity was assessed by inhibition of cyclooxygenase‐2 and 15‐lipoxygenase in vitro and inhibition of nitric oxide release in lipopolysaccharide‐induced BV‐2 cells. Antioxidant activity was assessed by DPPH and ABTS radical scavenging assays. The relationship between bioactivity and chemical fingerprints was then analyzed using chemometrics including gray relational analysis, bivariate correlation analysis, and orthogonal partial least squares analysis.ResultsDifferent batches of A. absinthium extracts possessed significant anti‐inflammatory and antioxidant activities to varying degrees. Eighty compounds were identified from A. absinthium, and 12 main common peaks were obtained from the UPLC fingerprints. P3 (chlorogenic acid), P5 (isochlorogenic acid A), and P6 (isochlorogenic acid C) were screened as the most promising active compounds by correlation analysis and further validated for their remarkable anti‐inflammatory effects.ConclusionThis is the first study to screen the quality markers of A. absinthium by establishing the spectrum–effect relationship, which can provide a reference for the development of quality standards and further research on A. absinthium.

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Toward the bioactive potential of myricitrin in food production: state-of-the-art green extraction and trends in biosynthesis

Geng

Xie

et al. 2023

Critical Reviews in Food Science and Nutrition

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Campomanesia lineatifolia Ruiz & Pavón (Myrtaceae): Isolation of major and minor compounds of phenolic-rich extract by high-speed countercurrent chromatography and anti-inflammatory evaluation

Cited by 4 publications

References 72 publications

Isolation of Momilactones A and B from Rice Husk Using High-Speed Countercurrent Chromatography

Isolation of Momilactones A and B from Rice Husk Using High-Speed Countercurrent Chromatography

Exploring anti‐inflammatory and antioxidant‐related quality markers of Artemisia absinthium L. based on spectrum–effect relationship

Toward the bioactive potential of myricitrin in food production: state-of-the-art green extraction and trends in biosynthesis

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