Camelina sativa: An Emerging Biofuel Crop

Chaturvedi, Shivani; Bhattacharya, Amrik; Khare, Sunil Kumar; Kaushik, Geetanjali

doi:10.1007/978-3-319-73645-7_110

Cited by 9 publications

(11 citation statements)

References 179 publications

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“…Although embryos of C. sativa are larger than Arabidopsis and are therefore more amenable to biochemical analyses, they are small enough that nutrient and/or oxygen gradients are likely to be small compared with larger embryos (Borisjuk et al, 2004), especially in culture. As a crop its advantages include a short life cycle that allows it to be grown between winter and summer crops (Allen et al, 2014;Chaturvedi et al, 2018), resilience to water and nutrient stresses (Iskandarov et al, 2014), the composition of its seed oil (Moser, 2010;Berti et al, 2016), and a relatively low susceptibility to disease and insect pests in its growth range (Vollmann and Eynck, 2015).…”

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confidence: 99%

High Flux Through the Oxidative Pentose Phosphate Pathway Lowers Efficiency in Developing Camelina Seeds

et al. 2019

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Many seeds are green during development, and light has been shown to play a role in the efficiency with which maternally supplied substrates are converted into storage compounds. However, the effects of light on the fluxes through central metabolism that determine this efficiency are poorly understood. Here, we used metabolic flux analysis to determine the effects of light on central metabolism in developing embryos of false flax (Camelina sativa). Metabolic efficiency in C. sativa is of interest because, despite its growing importance as a model oilseed and engineering target and its potential as a biofuel crop, its yields are lower than other major oilseed species. Culture conditions under which steady-state growth and composition of developing embryos match those in planta were used to quantify substrate uptake and respiration rates. The carbon conversion efficiency (CCE) was 21% 6 3% in the dark and 42% 6 4% under high light. Under physiological illumination, the CCE (32% 6 2%) was substantially lower than in green and nongreen oilseeds studied previously. 13 C and 14 C isotopic labeling experiments were used together with computer-aided modeling to map fluxes through central metabolism. Fluxes through the oxidative pentose phosphate pathway (OPPP) were the principal source of CO 2 production and strongly negatively correlated with CCE across light levels. OPPP fluxes were greatly in excess of demand for NAD(P)H for biosynthesis and larger than those measured in other systems. Excess reductant appears to be dissipated via cyanide-insensitive respiration. OPPP enzymes therefore represent a potential target for increasing efficiency and yield in C. sativa.

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High Flux Through the Oxidative Pentose Phosphate Pathway Lowers Efficiency in Developing Camelina Seeds

et al. 2019

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“…However, the role of other camelina meal components, such as phospholipids, polyphenols, or specific fibers, cannot be excluded. 47,48 Involved mechanisms at the intestinal and systemic levels remain mostly speculative at this initial stage, but it may be hypothesized that some of the effects observed, including on the adipose tissue and blood parameters, result from an impact of phytoSL or their metabolites at the intestinal level.…”

Section: ■ Discussionmentioning

confidence: 99%

“…To our knowledge, the present study, provides the first evidence that a mixture of natural plant SL, essentially in the form of NH 2 GIPC, and provided in their original matrix, exerted some beneficial effects during the MetS onset. However, the role of other camelina meal components, such as phospholipids, polyphenols or specific fibers cannot be excluded (47,48) .…”

Section: Attenuation Ofmentioning

confidence: 99%

Intestinal Availability and Metabolic Effects of Dietary Camelina Sphingolipids during the Metabolic Syndrome Onset in Mice

Hermier

Lan

Tellier

et al. 2019

J. Agric. Food Chem.

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Sphingolipids appear as a promising class of components susceptible to prevent the onset of the metabolic syndrome (MetS). Gut availability and effects of Camelina sativa sphingolipids were investigated in a mouse model of dietary-induced metabolic syndrome. Seed meals from two Camelina sativa lines enriched respectively in C24-and C16-NH 2-glycosyl-inositolphosphoryl-ceramides (NH 2 GIPC) were used in hypercaloric diets. After 5 weeks on these two hypercaloric diets, two markers of the MetS were alleviated (adiposity and insulin resistance) as well as inflammation markers and colon barrier dysfunction. A more pronounced effect was observed with the C16-NH 2 GIPC-enriched HC diet, in particular for colon barrier function. Despite a lower digestibility, C16-NH 2 GIPC were more prevalent in the intestine wall. Sphingolipids provided as camelina meal can therefore counteract some deleterious effects of a hypercaloric diet in mice at the intestinal and systemic levels.Interestingly, these beneficial effects seem partly dependent on sphingolipid acyl chain length.

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“…Camelina seeds are approximately 50 times larger than those of Arabidopsis, and they are rich in triacylglycerols (TAGs) containing mainly long unsaturated FAs, including linoleic acid (C18:3), which are excellent sources of omega‐3 FAs (Berti et al., 2016; Gugel and Falk, 2006). Depending on the ecotype, Camelina oil may represent up to 40% of the total seed dry weight, which also contains high levels of vitamin E and antioxidants responsible for extending the lifetime of Camelina oil‐containing products (Berti et al., 2016; Chaturvedi et al., 2019; Malik et al., 2018). As in other plants, Camelina TAG synthesis starts with the synthesis of FAs in plastids (Voelker and Kinney, 2001).…”

Section: Introductionmentioning

confidence: 99%

Exploring Camelina sativa lipid metabolism regulation by combining gene co‐expression and DNA affinity purification analyses

Gómez-Cano¹,

Chu²,

Cruz‐Gomez³

et al. 2022

The Plant Journal

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Camelina (Camelina sativa) is an annual oilseed plant that is gaining momentum as a biofuel cover crop. Understanding gene regulatory networks is essential to deciphering plant metabolic pathways, including lipid metabolism. Here, we take advantage of a growing collection of gene expression datasets to predict transcription factors (TFs) associated with the control of Camelina lipid metabolism. We identified approximately 350 TFs highly co-expressed with lipid-related genes (LRGs). These TFs are highly represented in the MYB, AP2/ERF, bZIP, and bHLH families, including a significant number of homologs of well-known Arabidopsis lipid and seed developmental regulators. After prioritizing the top 22 TFs for further validation, we identified DNA-binding sites and predicted target genes for 16 out of the 22 TFs tested using DNA affinity purification followed by sequencing (DAP-seq). Enrichment analyses of targets supported the co-expression prediction for most TF candidates, and the comparison to Arabidopsis revealed some common themes, but also aspects unique to Camelina. Within the top potential lipid regulators, we identified CsaMYB1, CsaABI3AVP1-2, CsaHB1, CsaNAC2, CsaMYB3, and CsaNAC1 as likely involved in the control of seed fatty acid elongation and CsaABI3AVP1-2 and CsabZIP1 as potential regulators of the synthesis and degradation of triacylglycerols (TAGs), respectively. Altogether, the integration of co-expression data and DNA-binding assays permitted us to generate a high-confidence and short list of Camelina TFs involved in the control of lipid metabolism during seed development.

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Camelina sativa: An Emerging Biofuel Crop

Cited by 9 publications

References 179 publications

High Flux Through the Oxidative Pentose Phosphate Pathway Lowers Efficiency in Developing Camelina Seeds

High Flux Through the Oxidative Pentose Phosphate Pathway Lowers Efficiency in Developing Camelina Seeds

Intestinal Availability and Metabolic Effects of Dietary Camelina Sphingolipids during the Metabolic Syndrome Onset in Mice

Exploring Camelina sativa lipid metabolism regulation by combining gene co‐expression and DNA affinity purification analyses

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