CAM Induction in Mesembryanthemum crystallinum: Protein Expression

Schmitt, Jürgen; Höfner, R.; Abou-Mandour, A. A.; Vázquez–Moreno, Luz; Bohnert, Hans J.

doi:10.1007/978-3-642-74221-7_20

Cited by 10 publications

(4 citation statements)

References 18 publications

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“…1), being induced during stress (13). Accumulation of this protein correlates well with increases in enzyme activity (22). The band at 109 kD corresponds to the Ppc2 gene product on the basis of molecular weight derived from the predicted amino acid sequence.…”

Section: Resultsmentioning

confidence: 99%

Developmental Control of Crassulacean Acid Metabolism Inducibility by Salt Stress in the Common Ice Plant

Cushman¹,

Michalowski²,

Bohnert³

1990

Plant Physiol.

102

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Section: Resultsmentioning

confidence: 99%

Developmental Control of Crassulacean Acid Metabolism Inducibility by Salt Stress in the Common Ice Plant

Cushman¹,

Michalowski²,

Bohnert³

1990

Plant Physiol.

102

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“…During the induction of CAM by salt stress, certain enzymes such as PEPC, pyruvate Pi dikinase, and NADPmalic enzyme show increases in enzyme activity (Holtum and Winter, 1982) that are accompanied by substantial mass increases in the enzymes themselves (Schmitt et al, 1988). In contrast, despite a significant enhancement in enolase activity in leaves during stress treatment ( Fig.…”

Section: Discussionmentioning

confidence: 96%

Posttranscriptional and Posttranslational Control of Enolase Expression in the Facultative Crassulacean Acid Metabolism Plant Mesembryanthemum crystallinum L

1995

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“…The increase in steady-state transcript levels of NADP-ME in ice-plant leaf tissue during the transition from C3 photosynthesis to CAM strongly suggests that this gene is responsible in part for the increased malic-enzyme accumulation [48] and activity observed in salt-stressed M . crystullinum [8].…”

Section: G T T T a G T C A T C T A T C A G A C A T T T T A G A C T T mentioning

confidence: 99%

Characterization and expression of a NADP‐malic enzyme cDNA induced by salt stress from the facultative crassulacean acid metabolism plant, Mesembryanthemum crystallinum

Cushman

1992

European Journal of Biochemistry

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The facultative halophyte and crassulacean acid-metabolism plant, Mesembryanthemum crystallinum shifts from C3 photosynthesis to crassulacean acid metabolism when exposed to high-salt or drought conditions. To study the molecular basis of this metabolic transition, the expression of NADP+-dependent malic enzyme (NADP-ME), which catalyzes the decarboxylation of malate to release pyruvate and ( 2 0 2 , has been investigated. The complete nucleotide sequence of a full-length cDNA clone was determined and found to contain a single open reading frame encoding a 585-amino-acid polypeptide of 64284 Da. The ice plant ( M . crystallinurn) NADP-ME shares amino acid identities in the range 72.5-79.0% when compared to other higher-plant enzymes and is more closely related to C3 rather than C, forms of the enzyme. Genomic Southern-blot analysis of ice-plant DNA indicates that NADP-ME is encoded by a small gene family. Steady-state transcript levels increase 8 -10-fold in response to salt stress in the leaves. Transcript levels in roots are extremely low and are unaffected by salt-stress treatment. Nuclear run-on experiments, using isolated nuclei from leaf tissue, confirm that the accumulation of NADP-ME transcripts is, in part, the result of increased transcriphon of this gene during salt stress.Crassulacean acid metabolism (CAM), a specialized mode of photosynthetic carbon assimilation, is a functional adaptation to arid environments and a C0,-concentrating mechanism [l].Phosphoenolpyruvate carboxylase catalyzes the primary, nocturnal fixation of atmospheric COz into oxaloacetate, which is subsequently reduced to malate and stored in the vacuole. During the day, plants close their stomata to avoid evaporative water loss and draw upon the stored malate as a carbon source. Malate is decarboxylated to form pyruvate and C02. Pyruvate is phosphorylated by pyruvate orthophosphate dikinase to phosphoenolpyruvate which enters the gluconeogenic pathway. The released CO, is refixed by ribulosebisphosphate carboxylase/oxygenase to enter the reductive pentose-phosphate pathway.

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CAM Induction in Mesembryanthemum crystallinum: Protein Expression

Cited by 10 publications

References 18 publications

Developmental Control of Crassulacean Acid Metabolism Inducibility by Salt Stress in the Common Ice Plant

Developmental Control of Crassulacean Acid Metabolism Inducibility by Salt Stress in the Common Ice Plant

Posttranscriptional and Posttranslational Control of Enolase Expression in the Facultative Crassulacean Acid Metabolism Plant Mesembryanthemum crystallinum L

Characterization and expression of a NADP‐malic enzyme cDNA induced by salt stress from the facultative crassulacean acid metabolism plant, Mesembryanthemum crystallinum

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