Developmental Control of Crassulacean Acid Metabolism Inducibility by Salt Stress in the Common Ice Plant

Cushman, John C.; Michalowski, Christine B.; Bohnert, Hans J.

doi:10.1104/pp.94.3.1137

Cited by 102 publications

(71 citation statements)

References 30 publications

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“…1C). Similar posttranscriptional stabilization has been observed previously in salt stressregulated genes such as PEPCase (Cushman et al, 1990), AtP5R (Hua et al, 2001), and SOS1 (Shi et al, 2003;Chung et al, 2008) and in abscisic acid-and water stress-regulated genes such as a-amylase/subtilisin inhibitor (BASI; Liu and Hill, 1995), le25, and his1-s (Cohen et al, 1999). Taken together, our results suggest that the SMD of RbcS1 and Cab1 as well as the control of the stress-inducible Dip1 and SalT genes are posttranscriptional events.…”

Section: Smd Of Photosynthetic Genes During Stress Conditionssupporting

confidence: 70%

Posttranscriptional Control of Photosynthetic mRNA Decay under Stress Conditions Requires 3′ and 5′ Untranslated Regions and Correlates with Differential Polysome Association in Rice

et al. 2012

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Abiotic stress, including drought, salinity, and temperature extremes, regulates gene expression at the transcriptional and posttranscriptional levels. Expression profiling of total messenger RNAs (mRNAs) from rice (Oryza sativa) leaves grown under stress conditions revealed that the transcript levels of photosynthetic genes are reduced more rapidly than others, a phenomenon referred to as stress-induced mRNA decay (SMD). By comparing RNA polymerase II engagement with the steady-state mRNA level, we show here that SMD is a posttranscriptional event. The SMD of photosynthetic genes was further verified by measuring the half-lives of the small subunit of Rubisco (RbcS1) and Chlorophyll a/b-Binding Protein1 (Cab1) mRNAs during stress conditions in the presence of the transcription inhibitor cordycepin. To discern any correlation between SMD and the process of translation, changes in total and polysome-associated mRNA levels after stress were measured. Total and polysome-associated mRNA levels of two photosynthetic (RbcS1 and Cab1) and two stress-inducible (Dehydration Stress-Inducible Protein1 and Salt-Induced Protein) genes were found to be markedly similar. This demonstrated the importance of polysome association for transcript stability under stress conditions. Microarray experiments performed on total and polysomal mRNAs indicate that approximately half of all mRNAs that undergo SMD remain polysome associated during stress treatments. To delineate the functional determinant(s) of mRNAs responsible for SMD, the RbcS1 and Cab1 transcripts were dissected into several components. The expressions of different combinations of the mRNA components were analyzed under stress conditions, revealing that both 39 and 59 untranslated regions are necessary for SMD. Our results, therefore, suggest that the posttranscriptional control of photosynthetic mRNA decay under stress conditions requires both 39 and 59 untranslated regions and correlates with differential polysome association.

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Section: Smd Of Photosynthetic Genes During Stress Conditionssupporting

confidence: 70%

Posttranscriptional Control of Photosynthetic mRNA Decay under Stress Conditions Requires 3′ and 5′ Untranslated Regions and Correlates with Differential Polysome Association in Rice

et al. 2012

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“…The observation that weak CAM can develop in M. crystallinum grown in non-saline conditions has led to the suggestion that the occurrence of CAM in M. crystallinum is an inevitable developmental process that is accelerated by stresses such as salinity (Cushman et al 1990;Herppich et al 1992;Adams et al 1998;Lü ttge 2004). However, in this study, M. crystallinum grown for up to 63 days of age in the absence of added NaCl, by which time the plants had five side branches that supported ca.…”

Section: Discussionmentioning

confidence: 99%

The effects of salinity, crassulacean acid metabolism and plant age on the carbon isotope composition of Mesembryanthemum crystallinum L., a halophytic C3-CAM species

Winter

Holtum

2005

Planta

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The carbon isotope composition of the halophyte Mesembryanthemum crystallinum L. (Aizoaceae) changes when plants are exposed to environmental stress and when they shift from C 3 to crassulacean acid metabolism (CAM). We examined the coupling between carbon isotope composition and photosynthetic pathway by subjecting plants of different ages to salinity and humidity treatments. Whole shoot d 13 C values became less negative in plants that were exposed to 400 mM NaCl in the hydroponic solution. The isotopic change had two components: a direct NaCl effect that was greatest in plants still operating in the C 3 mode and decreased proportionally with increasing levels of dark fixation, and a second component related to the degree of CAM expression. Ignoring the presumably diffusionrelated NaCl effect on carbon isotope ratios results in an overestimation of nocturnal CO 2 gain in comparison to an isotope versus nocturnal CO 2 gain calibration established previously for C 3 and CAM species grown under well-watered conditions. It is widely taken for granted that the shift to CAM in M. crystallinum is partially under developmental control and that CAM is inevitably expressed in mature plants. Plants, cultivated under non-saline conditions and high relative humidity (RH) for up to 63 days, maintained diel CO 2 gas-exchange patterns and d 13 C values typical of C 3 plants. However, a weak CAM gas-exchange pattern and an increase in d 13 C value were observed in non-salt-treated plants grown at reduced RH. These observations are consistent with environmental control rather than developmental control of the induction of CAM in mature M. crystallinum under non-saline conditions.

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“…Induction of PEPCase mRNA, protein, and the CAM pathway has also been observed in old, well-watered plants (1,3,9,13 (9) and CAM induction (14) in well-watered plants.…”

mentioning

confidence: 88%

Induction of mRNA for Phosphoenolpyruvate Carboxylase Is Correlated with a Decrease in Shoot Water Content in Well-Irrigated Mesembryanthemum crystallinum

Schmitt

Piepenbrock²

1992

Plant Physiol.

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The abundance of mRNA specific for phosphoenolpyruvate carboxylase (PEPCase) was measured in leaves from wellwatered plants of Mesembryanthemum crystallinum. Plants grown side by side in pots of four different volumes (0.16, 0.74, 2.6, 6.5 liters) were compared. The time of increase in the steady-state level of PEPCase mRNA in well-watered plants was dependent on soil volume. The larger the pot, the later PEPCase transcripts were increased. PEPCase mRNA induction started when shoot water content decreased to well below 4000% of dry weight. No positive correlation with the developmental status of the plants could be found. The data indicate that PEPCase mRNA induction in well-watered plants up to 10 weeks of age is controlled environmentally rather than developmentally.

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Developmental Control of Crassulacean Acid Metabolism Inducibility by Salt Stress in the Common Ice Plant

Cited by 102 publications

References 30 publications

Posttranscriptional Control of Photosynthetic mRNA Decay under Stress Conditions Requires 3′ and 5′ Untranslated Regions and Correlates with Differential Polysome Association in Rice

Posttranscriptional Control of Photosynthetic mRNA Decay under Stress Conditions Requires 3′ and 5′ Untranslated Regions and Correlates with Differential Polysome Association in Rice

The effects of salinity, crassulacean acid metabolism and plant age on the carbon isotope composition of Mesembryanthemum crystallinum L., a halophytic C3-CAM species

Induction of mRNA for Phosphoenolpyruvate Carboxylase Is Correlated with a Decrease in Shoot Water Content in Well-Irrigated Mesembryanthemum crystallinum

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