Calycosin Inhibits the Migration and Invasion of Human Breast Cancer Cells by Down-Regulation of Foxp3 Expression

Li, Shuangxi; Wang, Yafei; Feng, Chan; Guoli, Wu; Yu, Yao; Tian, Jing

doi:10.1159/000485784

Cited by 33 publications

(29 citation statements)

References 30 publications

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“…Calycosin holds potential to cure oxidative stress, inflammation, and cancer as has been reported in an earlier study on Chinese herbal plant Radix astragalus by Gao et al [45]. Reported compound has also been documented to anticipate reduction in migration and invasion capacity of human breast cancer cells [46]. Similarly, Vidalenolone, a novel phenolic metabolite reported in this study was previously isolated as a natural antioxidant from the tropical red alga Vidalia sp [47].…”

Section: Identification Of Compounds By Lc-ms/mssupporting

confidence: 67%

Anticancer and anti-inflammatory perspectives of Pakistan’s indigenous berry Grewia asiatica Linn (Phalsa)

Qamar

Akhtar

Sestili

et al. 2020

JBR

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BACKGROUND: Berries indigenously grown in Asia are known for their diversified nutritional and health promoting properties. Establishing a link between berry consumption and their classical uses in health management however requires detailed research in exploring varied biochemical factors and their therapeutic role in averting risks of chronic disorders. OBJECTIVE: The present study was aimed at evaluating anti-inflammatory and anticancer responses of fruit extracts of Grewia asiatica locally known as Phalsa. METHODS: Dichloromethane, methanol and 50% hydro-methanolic fractions of fruit were evaluated for polyphenols characterization, quantification and antioxidant assays. Anti-inflammatory and anti-nociceptive responses of fruit extracts were evaluated in rats and mice models, respectively, and cytotoxic activities were measured against MCF-7, HeLa, HEp-2, NCI-H522, HEK-293 cancer cell cultures. RESULTS: Phenolics quantification and biological study data suggested 50% hydro-methanolic extracts as maximum carrier of flavonoids (7.92 mgQE/g), anthocyanins (8.1 mg/Kg) and tannins (187.2 mgGAE/g) that significantly (p < 0.05)resulted in higher oxidation inhibition (IC50 41.1 ug/ml), paw edema inhibition (68-74%) and pain mediation in neurogenic phase(31-62%) when administrated at the rate of 400 mg/kg b.w. Maximum cytotoxic activity of G. asiatica (50% hydromethanolic extracts) was observed against MCF-7 (IC50 34.9 ug/mL), HEp-2 (IC50 80.4 ug/mL) and NCI-H522 (IC50 73 ug/mL) cancer cell lines. LC-ESI-MS/MS characterization of hydro-alcoholic fractions bearing potent biological activities revealed Gallic acid, Ellagic acid, Quinic acid, Calycosin, Vidalenolone, Quercetin, Myricetin, Liquitrigenin and 6-aldehydo-isoophiopogonone. Human equivalent doses of the extracts calculated on the basis of total phenolic contents for anti-inflammatory and nociceptive assays were in range between 6.2-15.8 mg/kg b.w., and 3.1-7.9 mg/kg b.w., respectively.

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Section: Identification Of Compounds By Lc-ms/mssupporting

confidence: 67%

Anticancer and anti-inflammatory perspectives of Pakistan’s indigenous berry Grewia asiatica Linn (Phalsa)

Qamar

Akhtar

Sestili

et al. 2020

JBR

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“…As a natural phytoestrogen, the therapeutic values of calycosin have been increasingly exploited in array of human diseases (Guo, Liu, Zhao, Zhao, & Liu, ; N. Li et al, ; S. Li et al, ; Y. Zhou, Liu, Liu, & Lin, ). The cumulative evidence suggested the anticancer, antioxidation, anti‐inflammation, and neuroprotective properties of this compound.…”

Section: Discussionmentioning

confidence: 99%

Calycosin attenuates MPTP‐induced Parkinson's disease by suppressing the activation of TLR/NF‐κB and MAPK pathways

Yang

Jia

Zhang

et al. 2018

Phytotherapy Research

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Parkinson is the second common neurodegenerative disease. The characteristics of Parkinson's disease (PD) are the dopamin neurons loss caused by neuroinflammation responses. C alycosin, an isoflavone phytoestrogen isolated from Astragalus membranaceus, has multiple pharmacological activities, such as anti-inflammation, anti-tumor, and neuroprotective effects. However, it is unknown whether calycosin can mitigate PD symptoms. This study aims to explore whether calycosin can alleviate PD symptoms and the underlying mechanisms. PD was induced in mice by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) injection, and calycosin was given intracerebroventricularly to these mice. A cell model of nerve inflammation was established by BV2 microglia cells injected with lipopolysaccharide (LPS). The motor states were evaluated by stepping, whisker, and cylinder experiments. The states of dopaminergic neurons and microglia were detected by immunostainning of tyrosine hydroxylase and cluster of differentiation molecule 11b (CD11b). The expression levels of inflammatory factors were detected by qPCR. Toll-like receptor (TLR)/ nuclear factor kappa B (NF-κB) and mitogen-activated protein kinase (MAPK) pathways were investigated by western blot. We found that calycosin treatment mitigated the behavioral dysfunctions and inflammatory responses in MPTP-induced PD mice. The TLR/NF-κB and MAPK pathways in MPTP-induced PD mice were inhibited by calycosin treatment, which was coincident with experiments in LPS-induced BV2 cells. Above all, calycosin mitigates PD symptoms through TLR/NF-κB and MAPK pathways in mice and cell lines.KEYWORDS

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“…CG inhibited MMP activation, maintained the expression of Cav-1 and tight junction proteins, attenuated BBB disruption, reduced infarction volume and improved the neurological outcomes in cerebral ischemia-reperfusion injury 125 , 126 . Calycosin also demonstrated bioactivities of scavenging free radicals and inhibiting MMP-9 activity in other cellular or non-cellular systems 127 , 128 , 129 . Calycosin and calycosin-7- O -β- D -glucoside decreased the production of NO, O 2 − , and TNF-α in lipopolysaccharide (LPS)-stimulated microglial or RAW 264.7 macrophages 124 , 130 and attenuated the neurotoxicity induced by various pathological factors including LPS, glutamate, mongholicus and xanthine (XA)/xanthine oxidase (XO) 130 , 131 , 132 .…”

Section: Natural Active Compounds Targeting Onoo − mentioning

confidence: 99%

“…Active MMPs cleave tight junction proteins, including occludin, claudin, and ZO-1, leading to blood-brain barrier damage. Calycosin or Calycosin-7- O -β- D -glucoside targets on CAV-1 (Ref 125 ), NO (Ref 124 , 125 , 130 ), and MMPs (Ref 125 , 127 , 128 ); M charantia polysaccharide targets on NO (Ref 45 ) and ONOO − (Ref 45 ); Baicalin targets on NO (Ref 150 , 151 ), ONOO − (Ref 46 , 59 , 151 ), and MMPs (Ref 46 , 152 , 153 ); Chlorogenic acid targets on CAV-1 (Ref 181 ), NO (Ref 173 ), ONOO − (Ref 168 , 169 , 170 , 171 ), and MMPs (172, 175, 176); lutein targets on ONOO − (Ref 182 ); lycopene targets on ONOO − (Ref 187 , 188 , 189 ). Ref, reference.…”

Section: Figurementioning

confidence: 99%

Targeting RNS/caveolin-1/MMP signaling cascades to protect against cerebral ischemia-reperfusion injuries: potential application for drug discovery

Chen

et al. 2018

Acta Pharmacol Sin

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Reactive nitrogen species (RNS) play important roles in mediating cerebral ischemia-reperfusion injury. RNS activate multiple signaling pathways and participate in different cellular events in cerebral ischemia-reperfusion injury. Recent studies have indicated that caveolin-1 and matrix metalloproteinase (MMP) are important signaling molecules in the pathological process of ischemic brain injury. During cerebral ischemia-reperfusion, the production of nitric oxide (NO) and peroxynitrite (ONOO−), two representative RNS, down-regulates the expression of caveolin-1 (Cav-1) and, in turn, further activates nitric oxide synthase (NOS) to promote RNS generation. The increased RNS further induce MMP activation and mediate disruption of the blood-brain barrier (BBB), aggravating the brain damage in cerebral ischemia-reperfusion injury. Therefore, the feedback interaction among RNS/Cav-1/MMPs provides an amplified mechanism for aggravating ischemic brain damage during cerebral ischemia-reperfusion injury. Targeting the RNS/Cav-1/MMP pathway could be a promising therapeutic strategy for protecting against cerebral ischemia-reperfusion injury. In this mini-review article, we highlight the important role of the RNS/Cav-1/MMP signaling cascades in ischemic stroke injury and review the current progress of studies seeking therapeutic compounds targeting the RNS/Cav-1/MMP signaling cascades to attenuate cerebral ischemia-reperfusion injury. Several representative natural compounds, including calycosin-7-O-β-D-glucoside, baicalin, Momordica charantia polysaccharide (MCP), chlorogenic acid, lutein and lycopene, have shown potential for targeting the RNS/Cav-1/MMP signaling pathway to protect the brain in ischemic stroke. Therefore, the RNS/Cav-1/MMP pathway is an important therapeutic target in ischemic stroke treatment.

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Calycosin Inhibits the Migration and Invasion of Human Breast Cancer Cells by Down-Regulation of Foxp3 Expression

Cited by 33 publications

References 30 publications

Anticancer and anti-inflammatory perspectives of Pakistan’s indigenous berry Grewia asiatica Linn (Phalsa)

Anticancer and anti-inflammatory perspectives of Pakistan’s indigenous berry Grewia asiatica Linn (Phalsa)

Calycosin attenuates MPTP‐induced Parkinson's disease by suppressing the activation of TLR/NF‐κB and MAPK pathways

Targeting RNS/caveolin-1/MMP signaling cascades to protect against cerebral ischemia-reperfusion injuries: potential application for drug discovery

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