Calreticulin attenuated microwave radiation-induced human microvascular endothelial cell injury through promoting actin acetylation and polymerization

Abstract

Recent work reveals that actin acetylation modification has been linked to different normal and disease processes and the effects associated with metabolic and environmental stressors. Herein, we highlight the effects of calreticulin on actin acetylation and cell injury induced by microwave radiation in human microvascular endothelial cell (HMEC). HMEC injury was induced by high-power microwave of different power density (10, 30, 60, 100 mW/cm, for 6 min) with or without exogenous recombinant calreticulin. The cell injury was assessed by lactate dehydrogenase (LDH) activity and Cell Counting Kit-8 in culture medium, migration ability, intercellular junction, and cytoskeleton staining in HMEC. Western blotting analysis was used to detected calreticulin expression in cytosol and nucleus and acetylation of globular actin (G-actin). We found that HMEC injury was induced by microwave radiation in a dose-dependent manner. Pretreatment HMEC with calreticulin suppressed microwave radiation-induced LDH leakage and increased cell viability and improved microwave radiation-induced decrease in migration, intercellular junction, and cytoskeleton. Meanwhile, pretreatment HMEC with exogenous calreticulin upregulated the histone acetyltransferase activity and the acetylation level of G-actin and increased the fibrous actin (F-actin)/G-actin ratio. We conclude that exogenous calreticulin protects HMEC against microwave radiation-induced injury through promoting actin acetylation and polymerization.

Doxycycline Induces Apoptosis of Brucella Suis S2 Strain-Infected HMC3 Microglial Cells by Activating Calreticulin-Dependent JNK/p53 Signaling Pathway

“…It has been reported that the expression of CALR protein is increased in tumor cells, which is associated to increased cell death in different tumours ( Sun et al., 2017 ). Additional report indicates that the CALR plays a role in antiapoptosis and antigen presentation within host cell ( Xu et al., 2017 ). For example, the increased expression of CALR protein often results in decreased rates of neuronal apoptosis after cerebral ischemia ( Chen et al., 2015 ).…”

PCV cap proteins fused with calreticulin expressed into polymers in Escherichia coli with high immunogenicity in mice

“…Generally, immunogenic protein with high-molecular-weight can induce stronger immune response than low-molecular-weight protein [ 15 ]. Calreticulin (CRT) is a highly conserved endoplasmic reticulum luminal Ca 2+ -binding protein and found to be involved in cellular processes (e.g., calcium storage and chaperone function) [ 16 ].…”