Caloranaerobacter azorensis gen. nov., sp. nov., an anaerobic thermophilic bacterium isolated from a deep-sea hydrothermal vent.

Wéry, Nathalie; Moricet, Jean-Marc; Cueff-Gauchard, Valérie; Jean, Joelle; Pignet, Patricia; Lesongeur, Françoise; Cambon‐Bonavita, Marie‐Anne; Barbier, Georges

doi:10.1099/00207713-51-5-1789

Cited by 104 publications

(82 citation statements)

References 35 publications

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“…Cultures were incubated at 30 u C under aerobic conditions. The methods used for the determination of growth parameters were as reported by Wery et al (2001b). Growth was observed at 15-44 u C with optimum growth at 30 u C (see Supplementary Fig.…”

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confidence: 99%

Haliea rubra sp. nov., a member of the Gammaproteobacteria from the Mediterranean Sea

Urios

Intertaglia

Lesongeur

2009

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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A novel aerobic, Gram-negative bacterium, designated strain CM41_15a T , was isolated from the surface of coastal waters of the north-western Mediterranean Sea. Cells were non-motile straight rods that formed red colonies on marine agar medium. The G+C content of the genomic DNA was 65 mol %. Phylogenetic analysis of the 16S rRNA gene sequence placed the strain in the genus Haliea within the class Gammaproteobacteria. On the basis of the 16S rRNA gene sequence comparison and physiological and biochemical characteristics, strain CM41_15a T represents a novel species, for which the name Haliea rubra sp. nov. is proposed. The type strain is CM41_15a T (5DSM 19751 T 5CIP 109758 T 5MOLA 104 T ).Members of the genus Microbulbifer, proposed originally by González et al. (1997), have been isolated from various marine environments, including salt marshes, intertidal sediments and coastal waters. Major amounts of iso-15 : 0 and iso-17 : 1v9c as well as ubiquinone Q-8 were defined as chemical markers for this genus (Yoon et al., 2004). Two strains isolated from the north-western Mediterranean Sea were studied. On the basis of their 16S rRNA gene sequences, the strains were phylogenetically related to the genus Microbulbifer. Fatty acid analysis revealed that iso-15 : 0 and iso-17 : 1v9c were either not present or present at very low levels in both strains. These differences, in addition to 16S rRNA gene dissimilarities and other phenotypic characteristics, led to the proposal of two novel species of two new genera, Haliea salexigens DSM 19537 T (Urios et al., 2008a) and Melitea salexigens DSM 19753 T (Urios et al., 2008b). Recently, another strain was isolated from seawater collected from the same area from which H. salexigens and Melitea salexigens had been isolated. The 16S rRNA gene sequence of the strain had a similarity of 94 % with H. salexigens, its nearest relative. In this paper, we have characterized this isolate and show that it possesses characteristics that allow the proposition of a novel species of the genus Haliea.

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confidence: 99%

Haliea rubra sp. nov., a member of the Gammaproteobacteria from the Mediterranean Sea

Urios

Intertaglia

Lesongeur

2009

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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“…Cultures were incubated at 30 u C under aerobic conditions. Methods for the determination of growth parameters were as reported by Wery et al (2001b). Growth was observed at 10-40 uC, with optimum growth at 30 uC (see Supplementary Fig.…”

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confidence: 99%

Balneola vulgaris gen. nov., sp. nov., a member of the phylum Bacteroidetes from the north-western Mediterranean Sea

Urios

Agogué

Lesongeur

et al. 2006

International Journal of Systematic and Evolutionary Microbiology

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International audienceA novel aerobic, Gram-negative bacterium, named 13IX/A01/164 T , was isolated from surface waters in the coastal northwestern Mediterranean Sea. Cells were motile, straight rods, 2,5 mm long and 0,2 mm wide, and formed orange colonies on marine agar medium. The G+C content of the genomic DNA of strain 13IX/A01/164 T was 42 mol%. Phylogenetic analysis of the 16S rRNA gene sequence placed the strain in the phylum Bacteroidetes within the family Crenotrichaceae. On the basis of 16S rRNA gene sequence comparison and physiological and biochemical characteristics, this isolate represents a novel species of a new genus, for which the name Balneola vulgaris gen. nov., sp. nov. is proposed. The type strain of Balneola vulgaris is 13IX/A01/164 T (=DSM 17893T=CIP 109092T=OOB 256T)

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“…It is only recently that two heterotrophic thermophilic anaerobes belonging to the order Clostridiales have been isolated and characterized. Caloranaerobacter azorensis (Wery et al, 2001), isolated from a chimney sample, was ascribed as a member of cluster XII of the order Clostridiales, while Caminicella sporogenes (Alain et al, 2002b), isolated from entire tube samples of Alvinella pompejana attached to small fragments of chimney rocks, was ascribed as a member of cluster XI of the same order as defined by Collins et al (1994). Both micro-organisms are considered as thermophiles, growing at temperatures above 60 uC.…”

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confidence: 99%

“…The latter include members of the orders Thermotogales and Clostridiales. The order Clostridiales includes two thermophilic isolates: one, Caloranaerobacter azorensis (Wery et al, 2001), is from the Atlantic rise hydrothermal vent; the other, Caminicella sporogenes (Alain et al, 2002b), is from the East-Pacific Rise hydrothermal vent. They respectively belong to clusters XII and XI of the Clostridium subphylum.…”

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confidence: 99%

Clostridium caminithermale sp. nov., a slightly halophilic and moderately thermophilic bacterium isolated from an Atlantic deep-sea hydrothermal chimney

Brisbarre

Fardeau

Cueff-Gauchard

et al. 2003

International Journal of Systematic and Evolutionary Microbiology

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A strictly anaerobic, slightly halophilic and moderately thermophilic, sporulating rod designated strain DVird3 T was isolated from deep-sea hydrothermal vent samples collected at a depth of approximately 800 m on the Atlantic Ocean Ridge. Strain DVird3 T possessed a few laterally inserted flagella, had a DNA G+C content of 33?1 mol% and grew optimally at pH 6?6 and at 45˚C. Growth was observed at temperatures between 20 and 58˚C and at pH values between 5?8 and 8?2. The optimum NaCl concentration for growth was 3 % sea salt (30 g l "1 ); no growth was observed in the presence of 15 or 60 g sea salt l "1 . Strain DVird3 T is heterotrophic and utilizes some sugars and various single amino acids. Acetate was the main fatty acid detected from carbohydrate fermentation, together with H 2 and CO 2 . Gelatin was used as an energy source. It performed the Stickland reaction. Phylogenetically, strain DVird3 T branched with members of cluster XI of the order Clostridiales, with Clostridium halophilum as its closest relative (similarity of 94?6 %). On the basis of its phenotypic, genotypic and phylogenetic characteristics, strain DVird3 T (=DSM 15212 T =CIP 107654 T ) is proposed as the type strain of a novel species of the genus Clostridium, Clostridium caminithermale sp. nov.Deep-sea hydrothermal vents are inhabited by a wide range of microbial communities that comprise free-living microorganisms as well as micro-organisms living in association with invertebrates (Jeanthon, 2000). Among these freeliving micro-organisms are bacteria belonging to the e-subclass of the Proteobacteria, and the significance of their ecological role has been identified, mainly in molecular surveys (Haddad et al., 1995;Jeanthon, 2000;Reysenbach et al., 2000b;Corre et al., 2001). The recent isolation, from deep-sea hydrothermal vents, of moderately thermophilic and thermophilic members of the e-subclass of the Proteobacteria has demonstrated the ability of these microorganisms to grow autotrophically using elemental sulfur as a terminal electron acceptor (Campbell et al., 2001;Alain et al., 2002a). This trait of sulfur reduction has also been found to be a common physiological feature of the wellstudied, anaerobic, chemoautotrophic, thermophilic and hyperthermophilic micro-organisms (domain Archaea) isolated from these peculiar deep marine environments (Jeanthon, 2000). Besides chemoautotrophy and mixotrophy, chemoheterotrophy has also been recognized as a significant type of metabolism within the deep-sea hydrothermal vent anaerobic microbial community (Jeanthon, 2000). Among such trophic microbial groups inhabiting deep environments, little attention has been paid to anaerobic mesophiles and moderate thermophiles in comparison with thermophilic and hyperthermophilic micro-organisms from both domains (Bacteria and Archaea). Therefore, most microbiological studies performed so far have underlined the fact that thermophilic and hyperthermophilic archaea thrive in the hottest part of the ecosystem, with only a few thermophilic anaerobic members o...

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Caloranaerobacter azorensis gen. nov., sp. nov., an anaerobic thermophilic bacterium isolated from a deep-sea hydrothermal vent.

Cited by 104 publications

References 35 publications

Haliea rubra sp. nov., a member of the Gammaproteobacteria from the Mediterranean Sea

Haliea rubra sp. nov., a member of the Gammaproteobacteria from the Mediterranean Sea

Balneola vulgaris gen. nov., sp. nov., a member of the phylum Bacteroidetes from the north-western Mediterranean Sea

Clostridium caminithermale sp. nov., a slightly halophilic and moderately thermophilic bacterium isolated from an Atlantic deep-sea hydrothermal chimney

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