Balneola vulgaris gen. nov., sp. nov., a member of the phylum Bacteroidetes from the north-western Mediterranean Sea

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

Lesongeur

2009

A novel aerobic, Gram-negative bacterium, designated strain CM41_15a T , was isolated from the surface of coastal waters of the north-western Mediterranean Sea. Cells were non-motile straight rods that formed red colonies on marine agar medium. The G+C content of the genomic DNA was 65 mol %. Phylogenetic analysis of the 16S rRNA gene sequence placed the strain in the genus Haliea within the class Gammaproteobacteria. On the basis of the 16S rRNA gene sequence comparison and physiological and biochemical characteristics, strain CM41_15a T represents a novel species, for which the name Haliea rubra sp. nov. is proposed. The type strain is CM41_15a T (5DSM 19751 T 5CIP 109758 T 5MOLA 104 T ).Members of the genus Microbulbifer, proposed originally by González et al. (1997), have been isolated from various marine environments, including salt marshes, intertidal sediments and coastal waters. Major amounts of iso-15 : 0 and iso-17 : 1v9c as well as ubiquinone Q-8 were defined as chemical markers for this genus (Yoon et al., 2004). Two strains isolated from the north-western Mediterranean Sea were studied. On the basis of their 16S rRNA gene sequences, the strains were phylogenetically related to the genus Microbulbifer. Fatty acid analysis revealed that iso-15 : 0 and iso-17 : 1v9c were either not present or present at very low levels in both strains. These differences, in addition to 16S rRNA gene dissimilarities and other phenotypic characteristics, led to the proposal of two novel species of two new genera, Haliea salexigens DSM 19537 T (Urios et al., 2008a) and Melitea salexigens DSM 19753 T (Urios et al., 2008b). Recently, another strain was isolated from seawater collected from the same area from which H. salexigens and Melitea salexigens had been isolated. The 16S rRNA gene sequence of the strain had a similarity of 94 % with H. salexigens, its nearest relative. In this paper, we have characterized this isolate and show that it possesses characteristics that allow the proposition of a novel species of the genus Haliea.

mentioning

confidence: 99%

Haliea rubra sp. nov., a member of the Gammaproteobacteria from the Mediterranean Sea

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

Lesongeur

2009

“…Sequencing and phylogenetic analysis were performed according to Urios et al (2006). Briefly, sequences were analysed for nearest neighbours using BLAST (Altschul et al, 1997) on the GenBank database.…”

mentioning

confidence: 99%

Pedobacter tournemirensis sp. nov., isolated from a fault water sample of a deep Toarcian argillite layer

International Journal of Systematic and Evolutionary Microbiology

Magot

2013

Self Cite

A Gram-negative bacterium, designated TF5-37.2-LB10 T , was isolated from subsurface water of the Toarcian geological layer of Tournemire, France. Cells were non-motile straight rods that formed cream to light pink colonies on 10-fold diluted LB agar. Strain TF5-37.2-LB10 T contained menaquinone 7 and its major fatty acids were iso-C 15 : 0 , summed feature 3 (iso-C 15 : 0 2-OH and/ or C 16 : 1 v7c), iso-C 17 : 0 3-OH and iso-C 17 : 1 v9c. The G+C content of the genomic DNA was 46 mol%. Phylogenetic analysis of the 16S rRNA gene sequence placed strain TF5-37.2-LB10 T within the genus Pedobacter, family Sphingobacteriaceae. Pedobacter composti TR6-06 T and Pedobacter oryzae DSM 19973 T were the closest phylogenetic relatives (93.5 and 93.3 % 16S rRNA gene sequence similarity, respectively). On the basis of 16S rRNA gene sequence comparison and physiological and biochemical characteristics, strain TF5-37.2-LB10 T represents a novel species of the genus Pedobacter, for which the name Pedobacter tournemirensis sp. nov. is proposed. The type strain is

“…The 16S rRNA gene was amplified and sequenced as described by Agogué et al (2005). The sequence was analysed as described by Urios et al (2006). Strain CZ41_10a T was phylogenetically affiliated to the family Rhodospirillaceae in the phylum Proteobacteria (Fig.…”

mentioning

confidence: 99%

Thalassobaculum salexigens sp. nov., a new member of the family Rhodospirillaceae from the NW Mediterranean Sea, and emended description of the genus Thalassobaculum

Michotey

International Journal of Systematic and Evolutionary Microbiology

et al. 2010

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A novel Gram-negative bacteria, named CZ41_10a T , was isolated from coastal surface waters of the north-western Mediterranean Sea. Cells were motile, pleomorphic rods, 1.6 mm long and 0.7 mm wide and formed cream colonies on marine agar medium. The G+C content of the genomic DNA was 65 mol%. Phylogenetic analysis of 16S rRNA gene sequences placed the new isolate in the genus Thalassobaculum, a member of the family Rhodospirillaceae, class Alphaproteobacteria. Unlike Thalassobaculum litoreum CL-GR58 T , its closest relative, strain CZ41_10a T was unable to grow anaerobically and did not exhibit nitrate reductase activity. On the basis of DNA-DNA hybridization, fatty acid content and physiological and biochemical characteristics, this isolate represents a novel species for which the name Thalassobaculum salexigens sp. nov. is proposed. The type strain is CZ41_10a T (5DSM 19539 T 5CIP 109064 T 5MOLA 84 T ). An emended description of the genus Thalassobaculum is also given.Bacteria performing the many steps of the nitrogen cycle are of great interest to agricultural and environmental researchers. Among these organisms, members of the genus Azospirillum are well studied because of their association with plants and their ability to fix nitrogen. Three strains, close to species of the genus Azospirillum, were isolated from surface waters of the Mediterranean Sea. As they were putative novel taxa, an investigation into their taxonomy and characteristics was performed. Their taxonomic position and their environmental origin led to hypotheses regarding their possible involvement in the nitrogen cycle, as the marine nitrogen cycle and nitrogen flow between the oceans and the atmosphere are key processes (Capone & Knapp, 2007).Of the three new strains isolated, two have recently been described as members of a novel genus, Nisaea (Urios et al., 2008). The denitrification genes nirK and nosZ were found in Nisaea denitrificans whereas Nisaea nitritireducens possessed only nirK. The ability to denitrify was confirmed by culturing methods. The third strain, named CZ41_10a T , was not able to denitrify and did not possess nirK or nosZ genes. Strain CZ41_10a T was phylogenetically placed between the genera Nisaea and Azospirillum. Recently, Thalassobaculum litoreum, a novel species of a new genus belonging to the family Rhodospirillaceae, has been described (Zhang et al., 2008). The type strain shared 99 % 16S rRNA gene sequence similarity with strain CZ41_10a T , but DNA-DNA hybridization, DNA G+C content, fatty acid composition, physiological and biochemical characteristics showed numerous and important differences between Thalassobaculum litoreum and strain CZ41_10a T . In this paper, these different properties are described and it is demonstrated that strain CZ41_10a T represents a novel species of the genus Thalassobaculum.Seawater samples were collected in February 2004 at the SOLA station located in the bay of Banyuls-sur-Mer (42 u 299 N 3 u 089 E), France, at a depth of 3 m. Subsamples were spread on nutrient agar plates ...