Calmodulin, calbindin-D28k, calretinin and neurocalcin in rat olfactory bulb during postnatal development

Bastianelli, Enrico; Pochet, Roland

doi:10.1016/0165-3806(95)00073-m

Cited by 30 publications

(46 citation statements)

References 13 publications

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“…Previous studies suggest that calbindin-immunoreactivity is present in the bulb by P1 (Enderlin et al, 1987;Bastianelli and Pochet, 1995), although our observations suggest only rudimentary staining even at P10. This discrepancy could be either due to procedural differences or to the fact that many antisera against calbindin crossreact with calretinin , which is precociously expressed in the bulb.…”

Section: Calbindincontrasting

confidence: 95%

“…Of the three calcium-binding proteins, calretininimmunoreactivity appears earlier in all regions of the brain, including the bulb, where it is present at birth (Andressen et al, 1993;Bastianelli and Pochet, 1995). Our results support previous finding that calretinin-positive olfactory nerve bundles are common and often fill entire glomeruli, suggesting the convergence of receptor axons with strong calretinin expression Bastianelli and Pochet, 1994).…”

Section: Calretininsupporting

confidence: 90%

“…Furthermore, our results support studies demonstrating that calretinin- immunoreactive cells are prominent in the granule cell and glomerular layers and relatively sparse in the external plexiform layer. Most mitral cells are lightly immunoreactive (Jacobowitz and Winsky, 1991;Résibois and Rogers, 1992;Bastianelli and Pochet, 1995;Wouterlood and Hä rtig, 1995). While unilateral naris closure dramatically reduces the expression of calbindin-immunoreactivity cells in the glomerular layer, the manipulation has no effect on profile number, intensity, or size of calretinin-immunoreactive cells in either the glomerular or granule cell layers.…”

Section: Calretininmentioning

confidence: 96%

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Activity-dependent regulation of calcium-binding proteins in the developing rat olfactory bulb

1997

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Intracellular calcium, important in a variety of second messenger cascades, is regulated in part by calcium-binding proteins such as calretinin, parvalbumin, and calbindin. These proteins are highly concentrated in the rat main olfactory bulb and are localized in distinct neuronal populations. In the present study, postnatal expression was characterized immunohistochemically in normal rats and in rats with functional olfactory deprivation caused by unilateral naris closure, a manipulation that attenuates electrical activity in the bulb. Bulbs were examined from rats that had undergone naris closure or sham surgery on either postnatal day 1 (P1) or P30 and were allowed varying subsequent survival times. Each of the calcium-binding proteins showed both distinct patterns of early expression and differential susceptibility to olfactory restriction. For example, at P10, the densest immunoreactivity was observed for calretinin, a protein whose expression was the least affected by naris closure. After occlusion from P1-P30, there was a 30% reduction in the density of calbindin-immunoreactive profiles in the glomerular layer, and parvalbumin-immunoreactive profiles were reduced by 64% in the external plexiform layer. Unlike many other changes induced by deprivation, the effects of olfactory restriction on calbindin and parvalbumin expression were not age dependent: naris closure from P30-P60 caused similar substantial decreases in calbindin and parvalbumin immunoreactivities. These data demonstrate that the expression of calbindin and parvalbumin in rat bulb is regulated, in part, by afferent activity that is associated with full sensory experiences. The reductions of these calcium-binding proteins following olfactory deprivation are likely to be commensurate with altered control of intracellular calcium.

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Section: Calbindincontrasting

confidence: 95%

Section: Calretininsupporting

confidence: 90%

Section: Calretininmentioning

confidence: 96%

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Activity-dependent regulation of calcium-binding proteins in the developing rat olfactory bulb

1997

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“…no. PV-28, LOT 5.5-Calbindin-D-28k (CB)CabPneuMouse1:1000Immunoblot, immunohistochemistry; no cross-reactivity with other members of the EF hand family; result virtually identical to Brinon et al (1992), Bastianelli and Pochet (1995), Crespo et al (1997)Purified bovine kidney CB/anti-CB, Sigma, Saint Louis, USA, prod. no.…”

Section: Methodsmentioning

confidence: 89%

Hyperpolarization-activated and cyclic nucleotide-gated channels are differentially expressed in juxtaglomerular cells in the olfactory bulb of mice

2010

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In the olfactory bulb, input from olfactory receptor neurons is processed by neuronal networks before it is relayed to higher brain regions. In many neurons, hyperpolarization-activated and cyclic nucleotide-gated (HCN) channels generate and control oscillations of the membrane potential. Oscillations also appear crucial for information processing in the olfactory bulb. Four channel isoforms exist (HCN1–HCN4) that can form homo- or heteromers. Here, we describe the expression pattern of HCN isoforms in the olfactory bulb of mice by using a novel and comprehensive set of antibodies against all four isoforms. HCN isoforms are abundantly expressed in the olfactory bulb. HCN channels can be detected in most cell populations identified by commonly used marker antibodies. The combination of staining with marker and HCN antibodies has revealed at least 17 different staining patterns in juxtaglomerular cells. Furthermore, HCN isoforms give rise to an unexpected wealth of co-expression patterns but are rarely expressed in the same combination and at the same level in two given cell populations. Therefore, heteromeric HCN channels may exist in several cell populations in vivo. Our results suggest that HCN channels play an important role in olfactory information processing. The staining patterns are consistent with the possibility that both homomeric and heteromeric HCN channels are involved in oscillations of the membrane potential of juxtaglomerular cells.Electronic supplementary materialThe online version of this article (doi:10.1007/s00441-009-0904-9) contains supplementary material, which is available to authorized users.

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“…In Xenopus, too, the mitral cells are the first cells to differentiate in the olfactory bulb, appearing much earlier than interneurons (Fritz et al, 1996). Interestingly, in rat pups the mitral cells of the main olfactory bulb were also CR-ir (Bastianelli and Pochet, 1995), and similar results have been reported in developing bulbs of other mammals (Kakuta et al, 2001). In adult brown trout, however, most mitral-like cells exhibit faint or very faint immunoreactivity except in the proximity of a few glomerular regions, suggesting down-regulation of CR expression with age.…”

Section: Discussionmentioning

confidence: 99%

Distribution of calretinin during development of the olfactory system in the brown trout, Salmo trutta fario: Comparison with other immunohistochemical markers

Castro

Becerra

Anadón

et al. 2008

Journal of Chemical Neuroanatomy

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Calmodulin, calbindin-D28k, calretinin and neurocalcin in rat olfactory bulb during postnatal development

Cited by 30 publications

References 13 publications

Activity-dependent regulation of calcium-binding proteins in the developing rat olfactory bulb

Activity-dependent regulation of calcium-binding proteins in the developing rat olfactory bulb

Hyperpolarization-activated and cyclic nucleotide-gated channels are differentially expressed in juxtaglomerular cells in the olfactory bulb of mice

Distribution of calretinin during development of the olfactory system in the brown trout, Salmo trutta fario: Comparison with other immunohistochemical markers

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