Distribution of calretinin during development of the olfactory system in the brown trout, Salmo trutta fario: Comparison with other immunohistochemical markers

Castro, Albert; Becerra, Manuela; Anadón, Ramón; Manso, Marı́a Jesús

doi:10.1016/j.jchemneu.2008.03.005

Cited by 28 publications

(44 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Even in salmonids a spatial segregation of calretinin-positive glomeruli exists. However, they are present also in the ventral glomerular layer, probably because calretinin-expressing olfactory neurons belong to all morphological subtypes, spreading over more bulbar regions (Porteros et al, 1997;Castro et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

“…The aim of the present study was to investigate, in Poecilia reticulata, the molecular aspects of olfactory sensory neurons and their projections into the olfactory bulb, still unknown in microsmatic fish. In particular, we reported the absence of immunostaining for PGP 9.5 and the localization of calretinin, two neuronal markers of the olfactory epithelium of mammals (Bastianelli et al, 1995;Weiler and Benali, 2005) and fish (Porteros et al, 1997;Saito et al, 2004;Yamamoto et al, 2004;Castro et al, 2008), and the immunolabelling pattern of a novel marker for crypt cells, S100 protein (Germanà et al, 2004(Germanà et al, , 2007. In addition, we used lectin binding to evaluate the molecular characteristics of cell surface glycoconjugates, both in the epithelium and in the bulbs, and to reveal potential segregated central projections, as seen in other species (Riddle et al, 1993;Franceschini et al, 1999).…”

Section: Introductionmentioning

confidence: 98%

See 1 more Smart Citation

Immunohistochemical and Histochemical Characteristics of the Olfactory System of the Guppy, Poecilia reticulata (Teleostei, Poecilidae)

Bettini

Lazzari

Ciani

2009

The Anatomical Record

View full text Add to dashboard Cite

Olfaction in fish has been studied using preferentially macrosmatic species as models. In the present research, the labelling patterns of different neuronal markers and lectins were analyzed in the olfactory neurons and in their bulbar axonal endings in the guppy Poecilia reticulata, belonging to the group of microsmatic fish. We observed that calretinin immunostaining was confined to a population of olfactory receptor cells localized in the upper layers of the sensory mucosa, probably microvillous neurons innervating the lateral glomerular layer. Immunoreactivity for S100 proteins was mainly evident in crypt cells, but also in other olfactory cells belonging to subtypes projecting in distinct regions of the bulbs. Protein gene product 9.5 (PGP 9.5) was not detected in the olfactory system of the guppy. Lectin binding revealed the presence of N-acetylglucosamine and a-N-acetylgalactosamine residues in the glycoconjugates of numerous olfactory neurons ubiquitously distributed in the mucosa. The low number of sugar types detected suggested a reduced glycosidic variability that could be an index of restricted odorant discrimination, in concordance with guppy visual-based behaviors. Finally, we counted few crypt cells which were immunoreactive for S100 and calretinin. Crypt cells were more abundant in guppy females. This difference is in accordance with guppy gender-specific responses to pheromones. Cells immunoreactive to calretinin showed no evidence of ventral projections in the bulbs. We assumed the hypothesis that their odorant sensitivity is not strictly limited to pheromones or sexual signals in general.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

Immunohistochemical and Histochemical Characteristics of the Olfactory System of the Guppy, Poecilia reticulata (Teleostei, Poecilidae)

Bettini

Lazzari

Ciani

2009

The Anatomical Record

View full text Add to dashboard Cite

show abstract

“…Other findings in Tupaia belangeri suggest that calretinin influences the migration and differentiation of neurons which are associated with the terminal nerve . In contrast, calretininimmunoreactive migrating cells are absent from the terminal nerve in the brown trout (Salmo trutta fario; Castro et al, 2008).…”

Section: Terminal Nervementioning

confidence: 97%

“…Similarly, Shimp et al (2003) have investigated the ducts leading to the vomeronasal organ in order to identify differences regarding the perinatal functionality of the vomeronasal system in primates (Microcebus murinus), mice, and insectivora. Compared with the data available for adult animals, much less is known about the expression patterns and functions of calcium-binding proteins in the developing olfactory systems (e.g., Halpern and Martínez-Marcos, 2003: mammals, VNO;Castro et al, 2008: Salmo trutta fario).…”

Section: Calretininmentioning

confidence: 99%

Early development of the nervous system of the eutherian <i>Tupaia belangeri</i>

Knabe¹,

Washausen²

2015

Primate Biol.

View full text Add to dashboard Cite

Abstract. The longstanding debate on the taxonomic status of Tupaia belangeri (Tupaiidae, Scandentia, Mammalia) has persisted in times of molecular biology and genetics. But way beyond that Tupaia belangeri has turned out to be a valuable and widely accepted animal model for studies in neurobiology, stress research, and virology, among other topics. It is thus a privilege to have the opportunity to provide an overview on selected aspects of neural development and neuroanatomy in Tupaia belangeri on the occasion of this special issue dedicated to Hans-Jürg Kuhn. Firstly, emphasis will be given to the optic system. We report rather "unconventional" findings on the morphogenesis of photoreceptor cells, and on the presence of capillary-contacting neurons in the tree shrew retina. Thereafter, network formation among directionally selective retinal neurons and optic chiasm development are discussed. We then address the main and accessory olfactory systems, the terminal nerve, the pituitary gland, and the cerebellum of Tupaia belangeri. Finally, we demonstrate how innovative 3-D reconstruction techniques helped to decipher and interpret so-far-undescribed, strictly spatiotemporally regulated waves of apoptosis and proliferation which pass through the early developing forebrain and eyes, midbrain and hindbrain, and through the panplacodal primordium which gives rise to all ectodermal placodes. Based on examples, this paper additionally wants to show how findings gained from the reported projects have influenced current neuroembryological and, at least partly, medical research.

show abstract

“…Several ORN-related proteins except for OMP and GST have been identified and applied as molecular markers to study olfactory function, including olfactory receptors (ORs: Morinishi et al, 2007), and calretinin (calcium-binding protein: Castro et al, 2008) in the olfactory system of several other salmonid fish. However, a single ORN expresses only one allele of a given OR gene (Chess et al, 1994) although the regulation of OR gene expression is unclear in the salmon ORN at present.…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization and histochemical demonstration of salmon olfactory marker protein in the olfactory epithelium of lacustrine sockeye salmon (Oncorhynchus nerka)

Kudo

Doi

Ueda

et al. 2009

Comparative Biochemistry and Physiology Part A: Molecular &

View full text Add to dashboard Cite

Despite the importance of olfactory receptor neurons (ORNs) for homing migration, the expression of olfactory marker protein (OMP) is not well understood in ORNs of Pacific salmon (Genus Oncorhynchus). In this study, salmon OMP was characterized in the olfactory epithelia of lacustrine sockeye salmon (O. nerka) by molecular biological and histochemical techniques. Two cDNAs encoding salmon OMP were isolated and sequenced.These cDNAs both contained a coding region encoding 173 amino acid residues, and the molecular mass of the two proteins was calculated to be 19,581.17 and 19,387.11 Da, respectively. Both amino acid sequences showed marked homology (90%). The protein and nucleotide sequencing demonstrates the existence of high-level homology between 2 salmon OMPs and those of other teleosts. By in situ hybridization using a digoxigeninlabeled salmon OMP cRNA probe, signals for salmon OMP mRNA were observed preferentially in the perinuclear regions of the ORNs. By immunohistochemistry using a specific antibody to salmon OMP, OMP-immunoreactivities were noted in the cytosol of those neurons. The present study is the first to describe cDNA cloning of OMP in salmon olfactory epithelium, and indicate that OMP is a useful molecular marker for the detection of the ORNs in Pacific salmon.

show abstract

Distribution of calretinin during development of the olfactory system in the brown trout, Salmo trutta fario: Comparison with other immunohistochemical markers

Cited by 28 publications

References 61 publications

Immunohistochemical and Histochemical Characteristics of the Olfactory System of the Guppy, Poecilia reticulata (Teleostei, Poecilidae)

Immunohistochemical and Histochemical Characteristics of the Olfactory System of the Guppy, Poecilia reticulata (Teleostei, Poecilidae)

Early development of the nervous system of the eutherian <i>Tupaia belangeri</i>

Molecular characterization and histochemical demonstration of salmon olfactory marker protein in the olfactory epithelium of lacustrine sockeye salmon (Oncorhynchus nerka)

Contact Info

Product

Resources

About

Distribution of calretinin during development of the olfactory system in the brown trout, Salmo trutta fario: Comparison with other immunohistochemical markers

Cited by 28 publications

References 61 publications

Immunohistochemical and Histochemical Characteristics of the Olfactory System of the Guppy, Poecilia reticulata (Teleostei, Poecilidae)

Immunohistochemical and Histochemical Characteristics of the Olfactory System of the Guppy, Poecilia reticulata (Teleostei, Poecilidae)

Early development of the nervous system of the eutherian &lt;i&gt;Tupaia belangeri&lt;/i&gt;

Molecular characterization and histochemical demonstration of salmon olfactory marker protein in the olfactory epithelium of lacustrine sockeye salmon (Oncorhynchus nerka)

Contact Info

Product

Resources

About

Early development of the nervous system of the eutherian <i>Tupaia belangeri</i>